LOCUS       sequence-335892-        9083 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CFP(ST)_EPACdDEP _Y(UST) biosensor to measure realtime changes in 
            FRET reflecting fluctuations in cAMP levels in living cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9083)
  AUTHORS   Ponsioen B, Zhao J, Riedl J, Zwartkruis F, van der Krogt G, Zaccolo 
            M, Moolenaar WH, Bos JL, Jalink K
  TITLE     Detecting cAMP-induced Epac activation by fluorescence resonance 
            energy transfer: Epac as a novel cAMP indicator.
  JOURNAL   EMBO Rep. 2004 Dec . 5(12):1176-80.
  PUBMED    15550931
REFERENCE   2  (bases 1 to 9083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2004 Dec . 5(12):1176-80."
COMMENT     Sequence Label: Epac-S-H27
FEATURES             Location/Qualifiers
     source          1..9083
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(28..30,31..33,34..744)
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2980..2982,2983..2985,2986..3696)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        complement(3748..3766)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3749..3766)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3786..3803)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3792..4016
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4062..4490
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4149..4168)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4359..4380
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4499..4519)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4504..4833
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4684..4819
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4746..4765
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4900..5694
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4954..4973)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5564..5583
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5868..5989
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5905..5924)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5959..5978
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6038..6054)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6038..6054)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6051..6073)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6062..6078
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6086..6092,6093..6110,6111..6116))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6131..6152
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6269..6286)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6440..7028)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6520..6539)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7199..7990,7991..8059))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7822..7841
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8060..8164)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8239..8258)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8430..8809
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8810..9013
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8964..8984
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9058..9077
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9058..9076
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aagcttgccg ccaccatggc ggccgcaatg gtgagcaagg gcgaggagct gttcaccggg
       61 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      121 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc
      241 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      301 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacatcag ccacaacgtc
      481 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
      541 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      601 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
      661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      721 ctcggcatgg acgagctgta caagtccgga ctcagatctc gatatcagcc cgtgggaact
      781 catgagatgg aggaggagtt ggccgaagct gtggccctgc tctcccagcg ggggcctgac
      841 gccctgctca ctgtggcact tcgaaagccc ccaggtcagc gcacggatga agagctggac
      901 ctcatctttg aggagctgct gcacatcaag gctgtggccc acctctccaa ctcggtgaag
      961 cgagaattag cggctgttct gctctttgaa ccacacagca aggcagggac cgtgttgttc
     1021 agccaggggg acaagggcac ttcgtggtac attatctgga agggatctgt caacgtggtg
     1081 acccatggca aggggctggt gaccaccctg catgagggag atgattttgg acagctggct
     1141 ctggtgaatg atgcaccccg ggcagccacc atcatcctgc gagaagacaa ctgtcatttc
     1201 ctgcgtgtgg acaagcagga cttcaaccgt atcatcaagg atgtggaggc aaagaccatg
     1261 cggctggaag aacatggcaa agtggtgctg gtgctggaga gagcctctca gggcgccggc
     1321 ccttcccgac ccccaacccc aggcaggaac cggtatacag tgatgtctgg caccccagag
     1381 aagatcctag agcttctgtt ggaggccatg ggaccagatt ccagtgctca tgacccaaca
     1441 gagacattcc tcagcgactt cctcctgacc cacagggtct tcatgcccag cgcccaactc
     1501 tgcgctgccc ttctgcacca cttccatgtg gagcctgcgg gtggcagcga gcaggagcgc
     1561 agcacctacg tctgcaacaa gaggcagcag atcttgcggc tggtcagcca gtgggtggcc
     1621 ctgtatggct ccatgctcca cactgaccct gtggccacca gcttcctcca gaaactctca
     1681 gacctggtgg gcagggacac ccgactcagc aacctgctga gggagcagtg gccagagagg
     1741 cggcgatgcc acaggttgga gaatggctgt gggaatgcat ctcctcagat gaaggcccgg
     1801 aacttgcctg tttggctccc caaccaggac gagccccttc ctggcagcag ctgtgccatc
     1861 caagttgggg ataaagtccc ctatgacatc tgccggccag accactcagt gttgaccctg
     1921 cagctgcctg tgacagcctc cgtgagagag gtgatggcag cgttggccca ggaggatggc
     1981 tggaccaagg ggcaggtgct ggtgaaggtc aattctgcag gtgatgccat tggcctgcag
     2041 ccagatgccc gtggtgtggc cacatctctg gggctcaatg agcgtctctt tgttgtcaac
     2101 ccacaggaag cgcatgagct gatcccacac cctgaccagc tggggcccac tgtgggctct
     2161 gctgaggggc tggacctggt gagtgccaag gacctggcag gccagctgac ggaccacgac
     2221 tggagcctct tcaacagtat ccaccaggtg gagctgatcc actatgtgct gggcccccag
     2281 catctgcggg atgtcaccac cgccaacctg gagcgcttca tgcgccgctt caatgagctg
     2341 cagtactggg tggccaccga gctgtgtctc tgccccgtgc ccggcccccg ggcccagctg
     2401 ctcaggaagt tcattaagct ggcggcccac ctcaaggagc agaagaatct caattccttc
     2461 tttgccgtca tgtttggcct cagcaactcg gccatcagcc gcctagccca cacctgggag
     2521 cggctgcccc acaaagtccg gaagctgtac tccgccctcg agaggctgct ggatccctca
     2581 tggaaccacc gggtataccg actggccctc gccaagctct cccctcctgt catccccttc
     2641 atgccccttc ttctcaaaga catgaccttc attcatgagg gaaaccacac actagtggag
     2701 aatctcatca actttgagaa gatgagaatg atggccagag ccgcgcggat gctgcaccac
     2761 tgccgaagcc acaaccctgt gcctctctca ccactcagaa gccgagtttc ccacctccac
     2821 gaggacagcc aggtggcgag gatttccaca tgctcggagc agtccctgag cacccggagt
     2881 ccagccagca cctgggctta tgtccagcag ctgaaggtca ttgacaacca gcgggaactc
     2941 tcccgcctct cccgagagct ggagccagct agcgagctca tggtgagcaa gggcgaggag
     3001 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     3061 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     3121 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac
     3181 ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     3241 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     3301 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     3361 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     3421 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     3481 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     3541 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccaaa
     3601 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     3661 gccgggatca ctctcggcat ggacgagctg tacaagccga attccctcga ggttaacgcg
     3721 gcgcgcctct aatctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg
     3781 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     3841 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     3901 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     3961 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     4021 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     4081 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4141 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4201 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4261 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4321 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4381 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     4441 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     4501 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4561 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     4621 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     4681 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4741 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     4801 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     4861 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     4921 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     4981 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     5041 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     5101 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     5161 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     5221 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     5281 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     5341 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     5401 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     5461 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     5521 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     5581 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     5641 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     5701 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     5761 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     5821 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     5881 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5941 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     6001 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     6061 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     6121 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     6181 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6241 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6301 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6361 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6421 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6481 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6541 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6601 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6661 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6721 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     6781 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     6841 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     6901 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     6961 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7021 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7081 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7141 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7201 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7261 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7321 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7381 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7441 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7501 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7561 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     7621 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     7681 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     7741 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     7801 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     7861 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     7921 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     7981 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8041 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8101 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8161 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     8221 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     8281 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     8341 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     8401 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
     8461 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     8521 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     8581 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     8641 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     8701 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     8761 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     8821 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     8881 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     8941 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     9001 tataagcaga gctctctggc taactagaga acccactgct tactggctta tcgaaattaa
     9061 tacgactcac tatagggaga ccc
//
