LOCUS       sequence-335896-       10968 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HiLITR transcription factor with full-length PMP34 targeting 
            sequence (Peroxisome).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10968)
  AUTHORS   Coukos RW, Yao D, Sanchez ML, Strand ET, Olive ME, Udeshi ND, 
            Weissman JS, Carr SA, Bassik MC, Ting AY
  TITLE     An engineered transcriptional reporter of protein localization 
            identifies regulators of mitochondrial and ER membrane protein 
            trafficking in high-throughput CRISPRi screens.
  JOURNAL   eLife. 2021 Aug 20;10. pii: 69142. doi: 10.7554/eLife.69142.
  PUBMED    34414886
REFERENCE   2  (bases 1 to 10968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-08-20"; volume: "10. pii: 69142. doi"; pages: " 
            10.7554/eLife.69142"
COMMENT     Sequence Label: PMP34-hLOV-TEVcs-GAL4bd-VP64-V5
FEATURES             Location/Qualifiers
     source          1..10968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     147..166
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     400..417
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    534..555
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(570..575,576..593,594..600)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    608..624
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     613..635
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     632..648
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     632..648
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     667..687
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        669..687
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        715..941
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             942..1122
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1169..1294
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1787..2020
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2205..2249
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2424..2924
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2843..2861
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2936..3955
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    4012..4129
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4154..5332
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4385..5323
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5280..5300
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5339..5348
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6737..6757
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQM"
     CDS             6794..7231
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             7280..7300
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7316..7465
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             7472..7513
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7535..8123
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7588..7608)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8006..8017)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8195..8428
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8506..8627
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8543..8562)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8597..8616
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8667..8802
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8822..8841)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8823..8841)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8851..8868)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8851..8867)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8860..8882)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9009..9464
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9096..9115)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9306..9327
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9490..9594
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9595..9663,9664..10455)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9813..9832)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(10626..10968,1..246)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
       61 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
      121 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
      181 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
      241 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
      301 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
      361 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
      421 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
      481 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
      541 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
      601 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
      661 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
      721 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
      781 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct
      841 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
      901 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
      961 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     1021 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     1081 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     1141 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     1201 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     1261 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     1321 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     1381 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     1441 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1501 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1561 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     1621 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     1681 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1741 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     1801 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     1861 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     1921 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     1981 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     2041 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     2101 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     2161 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     2221 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     2281 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     2341 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     2401 acctcccaac cccgagggga ccattgcgcc ttttccaagg cagccctggg tttgcgcagg
     2461 gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca
     2521 cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc
     2581 cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc
     2641 ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg
     2701 gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc
     2761 gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt
     2821 gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg
     2881 cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccagggggta ccaccatgaa
     2941 aaagcctgaa ctcaccgcta cctctgtcga gaagtttctg atcgaaaagt tcgacagcgt
     3001 ctccgacctg atgcagctct ccgagggcga agaatctcgg gctttcagct tcgatgtggg
     3061 agggcgtgga tatgtcctgc gggtgaatag ctgcgccgat ggtttctaca aagatcgcta
     3121 tgtttatcgg cactttgcat ccgccgctct ccctattccc gaagtgcttg acattgggga
     3181 gttcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca ccttgcaaga
     3241 cctgcctgaa accgaactgc ccgctgttct ccagcccgtc gccgaggcca tggatgccat
     3301 cgctgccgcc gatcttagcc agaccagcgg gttcggccca ttcggacctc aaggaatcgg
     3361 tcaatacact acatggcgcg atttcatctg cgctattgct gatccccatg tgtatcactg
     3421 gcaaactgtg atggacgaca ccgtcagtgc ctccgtcgcc caggctctcg atgagctgat
     3481 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgccgatt tcggctccaa
     3541 caatgtcctg accgacaatg gccgcataac agccgtcatt gactggagcg aggccatgtt
     3601 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccctggt tggcttgtat
     3661 ggagcagcag acccgctact tcgagcggag gcatcccgag cttgcaggat ctcctcggct
     3721 ccgggcttat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
     3781 tttcgatgat gcagcttggg ctcagggtcg ctgcgacgca atcgtccggt ccggagccgg
     3841 gactgtcggg cgtacacaaa tcgcccgcag aagcgctgcc gtctggaccg atggctgtgt
     3901 ggaagtgctc gccgatagtg gaaacagacg ccccagcact cgtcctaggg caaaggatct
     3961 gcagtaatga gaattctaga tcttgagaca aatggcagta ttcatccaca attttaaaag
     4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     4141 cagggacccc gggggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     4201 cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt
     4261 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     4321 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     4381 acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg
     4441 cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt
     4501 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg
     4561 agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc
     4621 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac
     4681 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc
     4741 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag
     4801 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc
     4861 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc
     4921 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag
     4981 ctcaaaatga aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa
     5041 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc
     5101 caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg
     5161 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg
     5221 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct
     5281 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gagctagcgc
     5341 caccatggct tccgtgctgt cctacgaaag cctggtccac gccgtggccg gagccgtggg
     5401 aagcgtgaca gcaatgacag tgttttttcc cctggataca gctagacttc gacttcaggt
     5461 tgatgagaaa agaaaatcca aaactacaca catggtgctc ctggagatca ttaaagaaga
     5521 aggactcctg gcaccatatc gagggtggtt tccagtgatt tccagtctct gctgctccaa
     5581 ttttgtctat ttctacactt ttaatagcct caaagcactc tgggtcaaag gtcaacattc
     5641 taccactgga aaagatctgg tagttgggtt tgttgcagga gtggttaatg tgttgctaac
     5701 aactccactc tgggtggtaa acaccagact gaagcttcaa ggagcaaaat ttaggaatga
     5761 agacattgta ccaacaaact acaaaggtat cattgatgct tttcatcaga tcattcgcga
     5821 tgaaggaatc tcggctttat ggaatggcac atttccctca ttgctgttgg tcttcaatcc
     5881 tgccatccag ttcatgtttt atgaaggttt aaaacggcag cttttaaaga aacggatgaa
     5941 gctttcttcc ttggatgtgt tcatcattgg tgcagtagcc aaagcgattg ccaccacggt
     6001 gacctatccc ctgcagacgg tacagtcaat tctgaggttt gggcgtcata gactaaaccc
     6061 agaaaacaga acattgggaa gtcttcggaa tattctctat cttcttcacc aacgagtaag
     6121 acgttttgga ataatgggac tctacaaagg ccttgaagcc aaactgctgc agacagtcct
     6181 cactgctgct ctcatgttcc ttgtttatga gaaactgaca gctgccacct tcacagttat
     6241 ggggctgaag cgtgcacacc aacacatcga tgagctggcc gagaagctgg ccggcctgga
     6301 catcaacggt ggaaccggtg agtttcgggc aaccacactg gaacggatcg agaaaagctt
     6361 cgtgattact gatccgagac tgcctgacaa cccaatcatt tttgtgagcg attccttcct
     6421 gcagctgaca gaatattctc gggaagagat cctggggcgc aattgccgtt ttctgcaggg
     6481 acccgagaca gaccgtgcca ctgttcggaa aatcagagat gctattgaca accagactga
     6541 agtgaccgtt cagctgatca attataccaa gagcggcaag aagttctgga acgtgttcca
     6601 cctgcagccg atgcgcgatt ataagggcga cgtccagtac ttcattggcg tgcagctgga
     6661 tggcaccgaa cgtcttcatg gcgccgctga gcgtgaggcg gtctgcctgg tcaaaaagac
     6721 agcctttcag attgctgaga acctgtactt ccagatgggc ggaggatccg gtagcggagg
     6781 atcaggctcc tctaaacttt tgtcctctat agagcaagcc tgtgatatat gccgacttaa
     6841 gaaattgaag tgtagcaaag agaaaccgaa gtgtgcgaaa tgtttgaaaa acaattggga
     6901 gtgtaggtat tccccaaaaa ctaagagatc accactcacc agggctcatt tgactgaggt
     6961 tgagtcacgg ctggagagac tggagcagct gttcctcctg atatttccta gagaagattt
     7021 ggacatgata ctgaaaatgg attctttgca agacataaaa gcccttctga cggggctgtt
     7081 tgtccaggat aatgtcaaca aggatgcagt aactgatcgc ttggcttccg tagagacaga
     7141 catgccattg acactgcggc aacaccgaat ttctgctact tccagctcag aggaatcatc
     7201 caataagggt caaaggcagc tgaccgtttc gggaggaggt ggaagcggag gaggaggaag
     7261 cggaggagga ggtagcggac ctaagaaaaa gaggaaggtg gcggccgctg gatccgatgc
     7321 tttagacgat tttgacttag atatgcttgg ttcagacgcg ttagacgact tcgacctaga
     7381 catgttaggc tcagatgcat tggacgactt cgatttagat atgttgggct ccgatgccct
     7441 agatgacttt gatctagata tgctaggtag tggcaagccg atccctaacc ctctgctggg
     7501 cctggacagc acttaggaat tcgatatcat cggtaatcaa cctctggatt acaaaatttg
     7561 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     7621 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     7681 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     7741 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     7801 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     7861 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     7921 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     7981 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     8041 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     8101 ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta
     8161 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     8221 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     8281 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     8341 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     8401 ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt
     8461 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     8521 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     8581 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     8641 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     8701 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     8761 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     8821 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     8881 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     8941 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     9001 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     9061 cgtgaccgct acacttgcca gcgccatagc gcccgctcct ttcgctttct tcccttcctt
     9121 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     9181 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     9241 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     9301 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     9361 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     9421 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     9481 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     9541 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     9601 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     9661 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     9721 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     9781 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     9841 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     9901 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     9961 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
    10021 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
    10081 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
    10141 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
    10201 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
    10261 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
    10321 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
    10381 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
    10441 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
    10501 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
    10561 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    10621 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
    10681 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
    10741 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
    10801 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    10861 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    10921 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagccc
//
