LOCUS       sequence-335899-        9591 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ectopic expression of SAE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9591)
  AUTHORS   Coukos RW, Yao D, Sanchez ML, Strand ET, Olive ME, Udeshi ND, 
            Weissman JS, Carr SA, Bassik MC, Ting AY
  TITLE     An engineered transcriptional reporter of protein localization 
            identifies regulators of mitochondrial and ER membrane protein 
            trafficking in high-throughput CRISPRi screens.
  JOURNAL   eLife. 2021 Aug 20;10. pii: 69142. doi: 10.7554/eLife.69142.
  PUBMED    34414886
REFERENCE   2  (bases 1 to 9591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-08-20"; volume: "10. pii: 69142. doi"; pages: " 
            10.7554/eLife.69142"
COMMENT     Sequence Label: pLX208-SAE1
FEATURES             Location/Qualifiers
     source          1..9591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    121..142
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(157..162,163..180,181..187)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    195..211
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     200..222
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     219..235
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     219..235
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     254..274
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        256..274
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        302..528
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             529..709
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    756..881
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1374..1607
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1792..1836
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2011..2511
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2430..2448
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2523..3317
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGAPNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2577..2596)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3187..3206
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    3359..3476
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3501..4679
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3732..4670
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4627..4647
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4686..4695
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5745..6333
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5798..5818)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6216..6227)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6405..6638
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6716..6837
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6753..6772)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6807..6826
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6877..7012
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7032..7051)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7033..7051)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7061..7078)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7061..7077)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7070..7092)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7219..7674
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7306..7325)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7516..7537
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7700..7804
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7805..7873,7874..8665)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8023..8042)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8836..9424
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9325..9344
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(9578..9591,1..4)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
       61 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
      121 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
      181 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      241 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      301 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      361 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      421 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      481 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      541 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      601 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      661 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      721 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      781 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      841 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      901 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      961 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1021 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1081 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1141 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1201 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1261 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1321 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1381 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1441 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1501 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1561 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1621 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1681 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1741 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1801 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1861 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1921 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1981 cagacccacc tcccaacccc gaggggacca ttgcgccttt tccaaggcag ccctgggttt
     2041 gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg
     2101 tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg
     2161 ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg
     2221 gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc agacggacag
     2281 cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca
     2341 gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg
     2401 gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc
     2461 acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca gggggtacca
     2521 ccatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     2581 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     2641 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ccgaatgaac
     2701 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     2761 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     2821 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     2881 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     2941 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     3001 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     3061 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     3121 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     3181 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     3241 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     3301 ttgacgagtt cttctaagaa ttctagatct tgagacaaat ggcagtattc atccacaatt
     3361 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     3421 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     3481 tttattacag ggaccccggg ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
     3541 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc
     3601 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     3661 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     3721 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
     3781 gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc
     3841 ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc
     3901 gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc
     3961 ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg
     4021 ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg
     4081 gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt
     4141 cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg
     4201 gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa
     4261 ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg
     4321 cagggagctc aaaatgaagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac
     4381 aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg
     4441 cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg
     4501 gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc
     4561 cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt
     4621 tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag
     4681 ctagcgccac catggtggag aaggaggagg ctggcggcgg cattagcgag gaggaggcgg
     4741 cacagtatga ccggcagatc cgcctgtggg gactggaggc ccagaaacgg ctgcgggcct
     4801 ctcgggtgct tcttgtcggc ttgaaaggac ttggggctga aattgccaag aatctcatct
     4861 tggcaggagt gaaaggactg accatgctgg atcacgaaca ggtaactcca gaagatcccg
     4921 gagctcagtt cttgattcgt actgggtctg ttggccgaaa tagggctgaa gcctctttgg
     4981 agcgagctca gaatctcaac cccatggtgg atgtgaaggt ggacactgag gatatagaga
     5041 agaaaccaga gtcatttttc actcaattcg atgctgtgtg tctgacttgc tgctccaggg
     5101 atgtcatagt taaagttgac cagatctgtc acaaaaatag catcaagttc tttacaggag
     5161 atgtttttgg ctaccatgga tacacatttg ccaatctagg agagcatgag tttgtagagg
     5221 agaaaactaa agttgccaaa gttagccaag gagtagaaga tgggcccgac accaagagag
     5281 caaaacttga ttcttctgag acaacgatgg tcaaaaagaa ggtggtcttc tgccctgtta
     5341 aagaagccct ggaggtggac tggagcagtg agaaagcaaa ggctgctctg aagcgcacga
     5401 cctccgacta ctttctcctt caagtgctct taaagttccg tacagataaa ggaagagatc
     5461 ccagttctga tacatatgag gaagattctg agttgttgct ccagatacga aatgatgtgc
     5521 ttgactcact gggtattagt cctgacctgc ttcctgagga ctttgtcagg tactgcttct
     5581 ccgagatggc cccagtgtgt gcggtggttg gagggatttt ggcacaggaa attgtgaagg
     5641 ccctgtctca gcgggaccct cctcacaaca acttcttctt cttcgatggc atgaagggga
     5701 atgggattgt ggagtgcctt ggccccaagt aggatatcat cggtaatcaa cctctggatt
     5761 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     5821 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     5881 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     5941 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     6001 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     6061 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     6121 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     6181 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     6241 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     6301 cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa
     6361 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     6421 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     6481 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6541 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6601 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca
     6661 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     6721 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6781 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6841 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     6901 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     6961 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     7021 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     7081 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     7141 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     7201 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     7261 ttacgcgcag cgtgaccgct acacttgcca gcgccatagc gcccgctcct ttcgctttct
     7321 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     7381 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     7441 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     7501 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     7561 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     7621 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     7681 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7741 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     7801 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     7861 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     7921 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7981 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     8041 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     8101 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     8161 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     8221 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     8281 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     8341 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     8401 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     8461 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     8521 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8581 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8641 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     8701 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     8761 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     8821 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8881 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8941 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     9001 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     9061 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     9121 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     9181 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     9241 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     9301 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     9361 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     9421 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     9481 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     9541 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga g
//
