LOCUS       sequence-335912-        8762 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HiLITR protease with MAVS c-terminal tmd targeting information 
            (Mitochondria).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8762)
  AUTHORS   Coukos RW, Yao D, Sanchez ML, Strand ET, Olive ME, Udeshi ND, 
            Weissman JS, Carr SA, Bassik MC, Ting AY
  TITLE     An engineered transcriptional reporter of protein localization 
            identifies regulators of mitochondrial and ER membrane protein 
            trafficking in high-throughput CRISPRi screens.
  JOURNAL   eLife. 2021 Aug 20;10. pii: 69142. doi: 10.7554/eLife.69142.
  PUBMED    34414886
REFERENCE   2  (bases 1 to 8762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-08-20"; volume: "10. pii: 69142. doi"; pages: " 
            10.7554/eLife.69142"
COMMENT     Sequence Label: EGFP-uTEV1-MAVS
FEATURES             Location/Qualifiers
     source          1..8762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    25..43
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    61..79
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    96..114
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    132..150
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    168..186
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    203..221
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    239..257
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    319..436
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(649..693)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    878..1111
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1604..1729
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1776..1956
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1957..2183)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2211..2231)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2211..2229)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2250..2266)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2250..2266)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2263..2285)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2274..2290
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2298..2304,2305..2322,2323..2328))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2343..2364
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2481..2498)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2652..3240)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2732..2751)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3411..4202,4203..4271))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4034..4053
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4272..4343)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4343..4478
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4518..4639
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4529..4548)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4583..4602
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4711..4944
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             5149..5160
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5558..5578
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(5618..5626,5627..5662,5663..5665,5666..5701,
                     5702..5704,5705..5740,5741..5746,5747..6364))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(6374..6884)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6437..6455)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(join(7808..8518,8519..8521,8522..8524))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7808..8518,8519..8521,8522..8524))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7850..7871)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8199..8218
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8458..8479
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      8521..8530
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(8550..8574)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(8571..8609)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    8621..8639
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8657..8675
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8692..8710
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8728..8746
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gtacacgcct acctcgacat acgttctcta tcactgatag ggagtaaact cgacatacgt
       61 tctctatcac tgatagggat aaactcgaca tacgttctct atcactgata gggagtaaac
      121 tcgacatacg ttctctatca ctgataggga gtaaactcga catacgttct ctatcactga
      181 tagggagtaa actcgacatc gttctctatc actgataggg agtaaactcg acatacgttc
      241 tctatcactg atagggagta aactcgacat atcgattcgc ggccaaagtg gatctctgct
      301 gtccctgtaa taaacccgaa aattttgaat ttttgtaatt tgtttttgta attctttagt
      361 ttgtatgtct gttgctatta tgtctactat tctttcccct gcactgtacc ccccaatccc
      421 cccttttctt ttaaaattgt ggatgaatac tgccatttgt ctcgaggtcg agaattgtcc
      481 cctcggggtt gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt
      541 cactatagaa agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat
      601 gaataatttt atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca
      661 tttatctaat tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat
      721 taaggagtgt attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt
      781 gtgattccaa atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt
      841 ggtgcaaatg agttttccag agcaacccca aatccccagg agctgttgat cctttaggta
      901 tctttccaca gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa
      961 cagatgctgt tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga
     1021 caataattgt ctggcctgta ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc
     1081 tgctcccaag aacccaagga acaaagctcc tattcccact gctctttttt ctctctgcac
     1141 cactcttctc tttgccttgg tgggtgctac tcctaatggt tcaattttta ctactttata
     1201 tttatataat tcacttctcc aattgtccct catatctcct cctccaggtc tgaagatcag
     1261 cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc ttcctctatc ttgtctaaag
     1321 cttccttggt gtcttttatc tctatccttt gatgcacaca atagagggtt gctactgtat
     1381 tatataatga tctaagttct tctgatcctg tctgaaggga tggttgtagc tgtcccagta
     1441 tttgtctaca gccttctgat gtttctaaca ggccaggatt aactgcgaat cgttctagct
     1501 ccctgcttgc ccatactata tgttttaatt tatatttttt ctttccccct ggccttaacc
     1561 gaattttttc ccatcgcgat ctaattctcc cccgcttaat actgacgctc tcgcacccat
     1621 ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt actcaccagt cgccgcccct
     1681 cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt
     1741 tccctttcgc tttcaagtcc ctgttcgggc gccactgcta gagattttcc acactgacta
     1801 aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac acactacttg
     1861 aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag
     1921 ctcccaggct cagatctggt ctaaccagag agacccgttt atgtatcgag ctaggcactt
     1981 aaatacaata tctctgcaat gcggcaattc agtggttcgt ccaatccatg tcagacccgt
     2041 ctgttgcctt cctaataagg cacgatcgta ccaccttact tccaccaatc ggcatgcacg
     2101 gtgctttttc tctccttgta aggcatgttg ctaactcatc gttaccatgt tgcaagacta
     2161 caagagtatt gcataagact acattaagct tgcagctcca gcttttgttc cctttagtga
     2221 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     2281 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     2341 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     2401 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     2461 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     2521 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2581 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2641 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2701 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2761 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2821 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     2881 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2941 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3001 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3061 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     3121 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3181 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3241 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3301 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3361 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3421 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3481 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     3541 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     3601 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     3661 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3721 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     3781 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     3841 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     3901 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     3961 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     4021 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     4081 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     4141 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     4201 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     4261 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     4321 atgagcggat acatatttga aaggcctcca aaaaagcctc ctcactactt ctggaatagc
     4381 tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc
     4441 ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggata
     4501 gctagagcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     4561 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     4621 aagctgcaat aaacaagttc ctctcactct ctgatattca tttctttgca agttataaat
     4681 actgaataat aagatgacat gaactactac tgctagagat tttccacact gactaaaagg
     4741 gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca
     4801 ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc
     4861 aggctcagat ctggtctaac cagagagacc cagtacaagc aaaaagcaga tcttgtcttc
     4921 gttgggagtg aattagccct tccagtcccc ccttttcttt taaaaagtgg ctaagatcta
     4981 cagctgcctt gtaagtcatt ggtcttaaag gtacctctag tccggacgcc gaggcgaaac
     5041 aggcggggag gcggcccaaa gggagatccg actcgtctga gggcgaaggc gaagacgcgg
     5101 aagaggccgc agagccggca gcaggccgcg ggaaggaagg tccgctggat tgagggccga
     5161 agggacgtag cagaaggacg tcccgcgcag aatccaggtg gcaacacagg cgagcagcca
     5221 tggaaaggac gtcagcttcc ccgacaacac cacggaattg tcagtgccca acagccgagc
     5281 ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc gccgtggcaa tagggagggg
     5341 gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca atgccccaac cagtgggggt
     5401 tgcgtcagca aacacagtgc acaccacgcc acgttgcctg acaacgggcc acaactcctc
     5461 ataaagagac agcaaccagg atttatacaa ggaggagaaa atgaaagcca tacgggaagc
     5521 aatagcatga tacaaaggca ttaaagcagc gtatccacat agcgtaaaag gagcaacata
     5581 gttaagaata cgatatcttg gatcgatcct tacttagtta cccggggagc atgtcaaggt
     5641 caaaatcgtc aagagcgtca gcaggcagca tatcaaggtc aaagtcgtca agggcatcgg
     5701 ctgggagcat gtctaagtca aaatcgtcaa gggcgtcggt cggcccgccg ctttcgcact
     5761 ttagctgttt ctccaggcca catatgatta gttccaggcc gaaaaggaag gcaggttcgg
     5821 ctccctgccg gtcgaacagc tcaattgctt gtctcagaag tgggggcata gaatcggtgg
     5881 taggtgtctc tctttcctct tttgctactt gatgctcctg ttcctccaat acgcagccca
     5941 gtgtaaagtg gcccacggcg gacagagcgt acagtgcgtt ctccagggag aagccttgct
     6001 gacacaggaa cgcgagctga ttttccaggg tttcgtactg tttctctgtt gggcgggtgc
     6061 cgagatgcac tttagccccg tcgcgatgtg agaggagagc acagcggtat gacttggcgt
     6121 tgttccgcag aaagtcttgc catgactcgc cttccagggg gcagaagtgg gtatgatgcc
     6181 tgtccagcat ctcgattggc agggcatcga gcagggcccg cttgttcttc acgtgccagt
     6241 acagggtagg ctgctcaact cccagctttt gagcgagttt ccttgtcgtc aggccttcga
     6301 taccgacacc attgagtaat tccagagctc cgtttatgac tttgctcttg tccagtctag
     6361 acatggtgaa ttgctgggga gagaggtcgg tgattcggtc aacgagggag ccgactgccg
     6421 acgtgcgctc cggaggcttg cagaatgcgg aacaccgcgc gggcaggaac agggcccaca
     6481 ctaccgcccc acaccccgcc tcccgcaccg ccccttcccg gccgctgctc tcggcgcgcc
     6541 ctgctgagca gccgctattg gccacagccc atcgcggtcg gcgcgctgcc attgctccct
     6601 ggcgctgtcc gtctgcgagg gtactagtga gacgtgcggc ttccgtttgt cacgtccggc
     6661 acgccgcgaa ccgcaaggaa ccttcccgac ttaggggcgg agcaggaagc gtcgccgggg
     6721 ggcccacaag ggtagcggcg aagatccggg tgacgctgcg aacggacgtg aagaatgtgc
     6781 gagacccagg gtcggcgccg ctgcgtttcc cggaaccacg cccagagcag ccgcgtccct
     6841 gcgcaaaccc agggctgcct tggaaaaggc gcaaccccaa ccccggatga attcggccct
     6901 agtgcagacg ccgccggtac agcaccacca ggagtgtgac taccagcacc cctgtcacag
     6961 ccacctggag ccacagagcc ccaggtgagg gccttccact acctgagccg gaactgccgc
     7021 tcccctctcc actcccaggc ttaccagacc cagaagtaga gccgctaccc tcgcctgatc
     7081 cgggcttgcc agaacctgac gtggaaccga attccaccat gaaaacttta tggccccccc
     7141 acaatactga gtcagcattt aatcgccaac cactaaccca ctgctgcgcc tcctgatttg
     7201 tcaacaattc catgaagttt ttcggcacgc ttgtgaaata attgtttgtg ttggtgaaat
     7261 tcgatgctga gtgtatacca acaatgaacc catctctagt tgatactaat ggattgccac
     7321 actgcccatc cttggtttga atccaatgct tccagaatat gccatcagat gaagggaatg
     7381 tgcaactagt gtctgacacc atgctagaca tgctcttagt ttggaagttg gttgtcacaa
     7441 gacatatgcg ctcttccctt tgtggctctc taaatttcag cttttgagga aatggtggga
     7501 aatccttagg catgcgaata attatcatgt ccctcccatc aatgaggtgt tgttgcaaag
     7561 tcgtggtgtt cttgaccttg aatacaccat gtagtgattg gaccaacagt gttccattat
     7621 ttcttctaaa caagtgcttg tttgtaatga tgaagggacc aaatccaata ccatacaacg
     7681 atgttgtgtg cccatcagat tcattcgtca aatgacaaat ggtgctcgat atcgggttgt
     7741 aatcacgtgg tcccttaaac aagctttctc cgcctgaacc ggatccgcct gtgccagatc
     7801 caaggtcctt gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca
     7861 gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca
     7921 ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt
     7981 ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga
     8041 tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca
     8101 gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt
     8161 tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct
     8221 tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt
     8281 gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca
     8341 cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct
     8401 tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt
     8461 cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca
     8521 ccatggtggc gctagccaat tctccaggcg atctgacggt tcactaaacg agctctgctt
     8581 atataggcct cccaccgtac acgcctacct cgacatacgt tctctatcac tgatagggag
     8641 taaactcgac atacgttctc tatcactgat agggataaac tcgacatacg ttctctatca
     8701 ctgataggga gtaaactcga catacgttct ctatcactga tagggagtaa actcgacata
     8761 cg
//
