LOCUS       sequence-335913-        3811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pUC19 vector containing an insertion of the Chantico promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Hall JM, Kainth AS, Rowton MJ, Lu E, Haddad G, Keplinger AJ, 
            Scepanovic J , Perez-Cervantes C, Chan SSK, Piccirilli JA, Moskowitz
            IP, Ruthenburg AJ
  TITLE     The cardiac lncRNA Chantico directly regulates Cxcl1 chemokine 
            transcription
  JOURNAL   bioRxiv 2021.12.23.474040
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.12.23.474040"
COMMENT     Sequence Label: pUC19-Chantico
FEATURES             Location/Qualifiers
     source          1..3811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1151..1169)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1188..1204)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1188..1204)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1201..1223)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1212..1228
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1236..1242,1243..1260,1261..1266))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1281..1302
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1419..1436)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1590..2178)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1670..1689)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2349..3140,3141..3209))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2972..2991
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3210..3314)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3382..3400
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3438..3460)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3560..3579
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3773..3795
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3787..3804
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3788..3804
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatacgact cactataggg cgtcattcag taaatataaa tgcagatagc ctacgttctg
       61 aaagtggggt tctagggtcc cttggttagt tttaaaaatt caaagtcact ttgagttttg
      121 ttttgttttg ttttgtttta aagcttgaga gtggccgtgc agagcaaagt tgtccaatta
      181 agtttatatc agtgacagat gggcttatca gaccttcgct ctcagacagt agccatggaa
      241 cacttagctg ctgcctcccg aaagtgcaag cagaccagtt caggatctga agctttgttt
      301 tggtttgact taaatgtaag tgcaaacagt cgctgtgggg tgtgtgtgtg tgtgtgtgtg
      361 tgatactggc cgatgcaggc tgagagtagg ggtgtctcac tcctgactgc gtactccccc
      421 tctgttttac acaggcttat ttactgttac acggagactt ttcttcgaac tgttaaactc
      481 ttggctgagt ttgtgtggag tatgaggcaa atctctaacc gcaagcatgc agtggaaaga
      541 ttaggcatac ttttgaaacg agtttgtgta ccagatcata ttcttggctc aagaaagaaa
      601 aagaaagggg gctggggaaa ggcttgtggt tgctactctg ggaactaaag aattcaaaag
      661 gcttgttctc catgtttcgt agacacagac atctggaatt gttgttttaa aattacaagc
      721 aattgtgtat gctttgttac tgcaacactt attcagaggc aaggacatag gtgccactgt
      781 tgtcagaaag ccagatgtcc tttccttaaa ttggcctgcc tggctgctgg cgcctgggtg
      841 tcagcagaca ctttgggaat ctcagttagc ggtactgcat ggatatagtg catctgcttc
      901 tcacacttct gccaaccttg tgtattggtt tgcttctctc catggtgtta aaacaatgac
      961 tgaaagcgtc ttgggtaggg caagggtata tttagcttat agttgcacat gacagtccat
     1021 cgtcaaggga gagcatggcg ggaactcaag gactttgagg aggaactgaa gcacaggcat
     1081 agaggctgtt cctcactggc ttgctcccca gggcttgctc agctagctgt cttacagagc
     1141 ccagaacctc cctttagtga gggttaatta agcttggcgt aatcatggtc atagctgttt
     1201 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     1261 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     1321 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     1381 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     1441 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1501 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1561 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1621 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1681 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1741 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1801 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1861 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1921 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1981 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     2041 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2101 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2161 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2221 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2281 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2341 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2401 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     2461 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     2521 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     2581 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     2641 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     2701 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     2761 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     2821 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     2881 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     2941 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3001 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3061 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3121 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3181 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3241 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3301 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     3361 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
     3421 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     3481 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     3541 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     3601 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
     3661 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     3721 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     3781 cgacgttgta aaacgacggc cagtgaattc g
//
