LOCUS       sequence-335917-        6682 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter for mouse IRENE-div lncRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6682)
  AUTHORS   Hall JM, Kainth AS, Rowton MJ, Lu E, Haddad G, Keplinger AJ, 
            Scepanovic J , Perez-Cervantes C, Chan SSK, Piccirilli JA, Moskowitz
            IP, Ruthenburg AJ
  TITLE     The cardiac lncRNA Chantico directly regulates Cxcl1 chemokine 
            transcription
  JOURNAL   bioRxiv 2021.12.23.474040
REFERENCE   2  (bases 1 to 6682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.12.23.474040"
COMMENT     Sequence Label: pIRENE-div-Firefly
FEATURES             Location/Qualifiers
     source          1..6682
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2443..2474
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2501..2510
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2507..4159
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    4203..4324
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4214..4233)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4268..4287
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4572..4589)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4743..5331)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4823..4842)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5531..6322,6323..6391))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    6496..6544
                     /note="synthetic polyadenylation signal"
     misc_feature    6558..6649
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     6598..6617
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 tcgaggatat cgataaggat ccgagatata tagaattata tatatatgta tattctactt
       61 gtttctgggg actggactcg gggagcctca tatgctggtc aagtgctgag tcactttatt
      121 gagacatagt aagtctgaac tacaattaac agggtcataa gcatactgtc ccatgagtct
      181 ggacatgtgg aaaagctcgt tgaaagttcg gtgaaatata ggaaccgctg acattttaaa
      241 tgagcgcgtc cccgttagtc agtccagggt ctgtcacaga cttgaagaag tacatcttcc
      301 gcaggtttta tttgtatgtt tatggaaaac tcaaatagca gaatgtgcgg gttttttttt
      361 tcaaattctg tgtgcttctt tatatttttg cacctaaaat ttattgttag tttcttgctg
      421 ctcacacttt cctcgtgctt ttggtggtgg ggatatttcc aaaacgcaaa atttctcttt
      481 ggtcacttgg ggctgtgatt gatttgatgg atccgatggg agctgctttc cgacctctgc
      541 agcccttttc cttatctccg ttttctttcc cctcagctcc gggctccgca gtgccagtaa
      601 caaggtcctc cacctgccct gactgctgca aaccgctaca gcgctacagc ataaacggcg
      661 gcgctcaaag gtgggggctc tgccggttcc gaaggatcgc gtctcagaag gtgataattt
      721 gaaaaatcag gtgacattga agtgggtcga ggcttcaagg tgccttcaga gctctgctag
      781 cggtggcttg gagctctggg aagcggctct gcagacgctt tgggcttgag agctcagatt
      841 gagcgacgcg cagctgcggg ccattcgctc ctagcttcgc ggacgagccg ggtctccgag
      901 tgtgccgcgg gacgtggtca gagtccaaga gtcactgccc gaacctggtg gctgaaaccc
      961 aaccggaagc ctcggagcct ccgcctccct ccaggaccgc tggggtctcc ggtgggcatg
     1021 aatcaggttg taagtggtcg ctgcacagtc ccaggatcat cgctgcccct tctcccgctc
     1081 ctccccgccg caccatcttt ttctttcaga ccgtgcttta cagcccttaa tgtgcataaa
     1141 ataaactgtg tcccacggaa catgccgcct tctctcacgc gggccgagag gattgtgaac
     1201 cggtaggcgg caaagtgtca cacggggaaa gcccagacta cggactgctg ggaatctggg
     1261 tgtacccgga gcctccctgg gtggcccggg acttcgaggg gccacctctg ctcccacggg
     1321 attcggccag ggcacgaatt ggtgaaaagc gggatggaga cgctagagga acacaatgtc
     1381 acacgccaag ctttcaaggt gcacatgacg gctgcagaca ctcaagccct taaaaggagc
     1441 atttaaactt gggggggggg gagtacggat ggaactcaga ggcacaccaa ttagtgtgaa
     1501 cacaacactc gcggggactt tgcgggagcg ttcacaattc cggagtgcac ggggaccggg
     1561 aggtggctcc gccccgcctc gctccagtca aacttcagtc tctgccggta gaaaggcgtc
     1621 tctgcgctcc cagggatcta ctcgcgcgtc gcctgtacaa accctctgct gtgtggcctt
     1681 gtatctttag gtagggggac atcttttgtc tctcggcaag aagttttcct ccggggaaga
     1741 taaagtaatc gatagggcct ttttaaatag ctccgagttt cctgtcgggc caggagtatc
     1801 agcgcgcaca ccaaatctgc tctggtatgt catcttatct cccttccccg ctgttgtccc
     1861 caaacgctgc ctgtcaagag agacgccacc cgcattagga cccagactgg ggcccttccg
     1921 ctcggggtca cgcgctgccc cctcggctcc gctgtccttc gcgttcctgg agggtgggaa
     1981 ggagatgctg cagcccgcat gaagattctg ggacatggag cgggtcactg gggcacactg
     2041 tcactcagaa cgggtgtgtg tggaccgctt ttcaaccaca aacacaagga acagccaaac
     2101 agcgcattga ctgcttgtct gtgtgaagaa aaaccaggct cattcttcga aggggccatt
     2161 ggatctcaga gcgctccagg ctggccccag ctttccctaa ctccaggatg actttggaag
     2221 gggagaggag agggtctggc ctcagaatcc tgccgttttc ctttgcttca actgctccac
     2281 ttccaacctg cagggaaccg gagtggggtt gcttagccaa cacagaaagg ccaaggtagt
     2341 ggtgacaagt gaccaccgct tgggcagttc tttctttctt tctctctttc tttctttctt
     2401 tcttgatatc aagatctggc ctcggcggcc aagcttagac actagagggt atataatgga
     2461 agctcgactt ccagcttggc aatccggtac tgttggtaaa gccaccatgg aagatgccaa
     2521 aaacattaag aagggcccag cgccattcta cccactcgaa gacgggaccg ccggcgagca
     2581 gctgcacaaa gccatgaagc gctacgccct ggtgcccggc accatcgcct ttaccgacgc
     2641 acatatcgag gtggacatta cctacgccga gtacttcgag atgagcgttc ggctggcaga
     2701 agctatgaag cgctatgggc tgaatacaaa ccatcggatc gtggtgtgca gcgagaatag
     2761 cttgcagttc ttcatgcccg tgttgggtgc cctgttcatc ggtgtggctg tggccccagc
     2821 taacgacatc tacaacgagc gcgagctgct gaacagcatg ggcatcagcc agcccaccgt
     2881 cgtattcgtg agcaagaaag ggctgcaaaa gatcctcaac gtgcaaaaga agctaccgat
     2941 catacaaaag atcatcatca tggatagcaa gaccgactac cagggcttcc aaagcatgta
     3001 caccttcgtg acttcccatt tgccacccgg cttcaacgag tacgacttcg tgcccgagag
     3061 cttcgaccgg gacaaaacca tcgccctgat catgaacagt agtggcagta ccggattgcc
     3121 caagggcgta gccctaccgc accgcaccgc ttgtgtccga ttcagtcatg cccgcgaccc
     3181 catcttcggc aaccagatca tccccgacac cgctatcctc agcgtggtgc catttcacca
     3241 cggcttcggc atgttcacca cgctgggcta cttgatctgc ggctttcggg tcgtgctcat
     3301 gtaccgcttc gaggaggagc tattcttgcg cagcttgcaa gactataaga ttcaatctgc
     3361 cctgctggtg cccacactat ttagcttctt cgctaagagc actctcatcg acaagtacga
     3421 cctaagcaac ttgcacgaga tcgccagcgg cggggcgccg ctcagcaagg aggtaggtga
     3481 ggccgtggcc aaacgcttcc acctaccagg catccgccag ggctacggcc tgacagaaac
     3541 aaccagcgcc attctgatca cccccgaagg ggacgacaag cctggcgcag taggcaaggt
     3601 ggtgcccttc ttcgaggcta aggtggtgga cttggacacc ggtaagacac tgggtgtgaa
     3661 ccagcgcggc gagctgtgcg tccgtggccc catgatcatg agcggctacg ttaacaaccc
     3721 cgaggctaca aacgctctca tcgacaagga cggctggctg cacagcggcg acatcgccta
     3781 ctgggacgag gacgagcact tcttcatcgt ggaccggctg aagagcctga tcaaatacaa
     3841 gggctaccag gtagccccag ccgaactgga gagcatcctg ctgcaacacc ccaacatctt
     3901 cgacgccggg gtcgccggcc tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt
     3961 cgtgctggaa cacggtaaaa ccatgaccga gaaggagatc gtggactatg tggccagcca
     4021 ggttacaacc gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg
     4081 actgaccggc aagttggacg cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg
     4141 cggcaagatc gccgtgtaat aattctagag tcggggcggc cggccgcttc gagcagacat
     4201 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     4261 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     4321 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     4381 tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc cgtcgaccga
     4441 tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg
     4501 tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc
     4561 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     4621 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     4681 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     4741 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     4801 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     4861 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     4921 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     4981 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     5041 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     5101 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     5161 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     5221 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     5281 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     5341 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     5401 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     5461 aaatcaatct aaagtatata tgagtaaact tggtctgaca gcggccgcaa atgctaaacc
     5521 actgcagtgg ttaccagtgc ttgatcagtg aggcaccgat ctcagcgatc tgcctatttc
     5581 gttcgtccat agtggcctga ctccccgtcg tgtagatcac tacgattcgt gagggcttac
     5641 catcaggccc cagcgcagca atgatgccgc gagagccgcg ttcaccggcc cccgatttgt
     5701 cagcaatgaa ccagccagca gggagggccg agcgaagaag tggtcctgct actttgtccg
     5761 cctccatcca gtctatgagc tgctgtcgtg atgctagagt aagaagttcg ccagtgagta
     5821 gtttccgaag agttgtggcc attgctactg gcatcgtggt atcacgctcg tcgttcggta
     5881 tggcttcgtt caactctggt tcccagcggt caagccgggt cacatgatca cccatattat
     5941 gaagaaatgc agtcagctcc ttagggcctc cgatcgttgt cagaagtaag ttggccgcgg
     6001 tgttgtcgct catggtaatg gcagcactac acaattctct taccgtcatg ccatccgtaa
     6061 gatgcttttc cgtgaccggc gagtactcaa ccaagtcgtt ttgtgagtag tgtatacggc
     6121 gaccaagctg ctcttgcccg gcgtctatac gggacaacac cgcgccacat agcagtactt
     6181 tgaaagtgct catcatcggg aatcgttctt cggggcggaa agactcaagg atcttgccgc
     6241 tattgagatc cagttcgata tagcccactc ttgcacccag ttgatcttca gcatctttta
     6301 ctttcaccag cgtttcgggg tgtgcaaaaa caggcaagca aaatgccgca aagaagggaa
     6361 tgagtgcgac acgaaaatgt tggatgctca tactcgtcct ttttcaatat tattgaagca
     6421 tttatcaggg ttactagtac gtctctcaag gataagtaag taatattaag gtacgggagg
     6481 tattggacag gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg
     6541 tgtgaatcga tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta
     6601 gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa catttctctg gcctaactgg
     6661 ccggtacctg agctcgctag cc
//
