LOCUS       sequence-335919-        9101 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CFP(UST)_EPACdDEPCD_cp173Venus(ST) biosensor to measure realtime 
            changes in FRET reflecting fluctuations in cAMP levels in living 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9101)
  AUTHORS   van der Krogt GN, Ogink J, Ponsioen B, Jalink K
  TITLE     A comparison of donor-acceptor pairs for genetically encoded FRET 
            sensors: application to the Epac cAMP sensor as an example.
  JOURNAL   PLoS ONE. 2008  . 3(4):e1916.
  PUBMED    18382687
REFERENCE   2  (bases 1 to 9101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE. 
            2008  . 3(4):e1916."
COMMENT     Sequence Label: Epac-S-H90
FEATURES             Location/Qualifiers
     source          1..9101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(28..30,31..33,34..744)
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             2980..3714
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     promoter        complement(3766..3784)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3767..3784)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3804..3821)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3810..4034
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4080..4508
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4167..4186)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4377..4398
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4517..4537)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4522..4851
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4702..4837
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4764..4783
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4918..5712
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4972..4991)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5582..5601
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5886..6007
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5923..5942)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5977..5996
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6056..6072)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6056..6072)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6069..6091)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6080..6096
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6104..6110,6111..6128,6129..6134))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6149..6170
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6287..6304)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6458..7046)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6538..6557)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7217..8008,8009..8077))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7840..7859
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8078..8182)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8257..8276)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8448..8827
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8828..9031
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8982..9002
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9076..9095
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9076..9094
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aagcttgccg ccaccatggc ggccgcaatg gtgagcaagg gcgaggagct gttcaccggg
       61 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      121 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc
      241 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      301 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacatcag ccacaacgtc
      481 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
      541 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      601 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
      661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      721 ctcggcatgg acgagctgta caagtccgga ctcagatctc gatatcagcc cgtgggaact
      781 catgagatgg aggaggagtt ggccgaagct gtggccctgc tctcccagcg ggggcctgac
      841 gccctgctca ctgtggcact tcgaaagccc ccaggtcagc gcacggatga agagctggac
      901 ctcatctttg aggagctgct gcacatcaag gctgtggccc acctctccaa ctcggtgaag
      961 cgagaattag cggctgttct gctctttgaa ccacacagca aggcagggac cgtgttgttc
     1021 agccaggggg acaagggcac ttcgtggtac attatctgga agggatctgt caacgtggtg
     1081 acccatggca aggggctggt gaccaccctg catgagggag atgattttgg acagctggct
     1141 ctggtgaatg atgcaccccg ggcagccacc atcatcctgc gagaagacaa ctgtcatttc
     1201 ctgcgtgtgg acaagcagga cttcaaccgt atcatcaagg atgtggaggc aaagaccatg
     1261 cggctggaag aacatggcaa agtggtgctg gtgctggaga gagcctctca gggcgccggc
     1321 ccttcccgac ccccaacccc aggcaggaac cggtatacag tgatgtctgg caccccagag
     1381 aagatcctag agcttctgtt ggaggccatg ggaccagatt ccagtgctca tgacccaaca
     1441 gagacattcc tcagcgactt cctcctgacc cacagggtct tcatgcccag cgcccaactc
     1501 tgcgctgccc ttctgcacca cttccatgtg gagcctgcgg gtggcagcga gcaggagcgc
     1561 agcacctacg tctgcaacaa gaggcagcag atcttgcggc tggtcagcca gtgggtggcc
     1621 ctgtatggct ccatgctcca cactgaccct gtggccacca gcttcctcca gaaactctca
     1681 gacctggtgg gcagggacac ccgactcagc aacctgctga gggagcagtg gccagagagg
     1741 cggcgatgcc acaggttgga gaatggctgt gggaatgcat ctcctcagat gaaggcccgg
     1801 aacttgcctg tttggctccc caaccaggac gagccccttc ctggcagcag ctgtgccatc
     1861 caagttgggg ataaagtccc ctatgacatc tgccggccag accactcagt gttgaccctg
     1921 cagctgcctg tgacagcctc cgtgagagag gtgatggcag cgttggccca ggaggatggc
     1981 tggaccaagg ggcaggtgct ggtgaaggtc aattctgcag gtgatgccat tggcctgcag
     2041 ccagatgccc gtggtgtggc cacatctctg gggctcaatg agcgtctctt tgttgtcaac
     2101 ccacaggaag cgcatgagct gatcccacac cctgaccagc tggggcccac tgtgggctct
     2161 gctgaggggc tggacctggt gagtgccaag gacctggcag gccagctgac ggaccacgac
     2221 tggagcctct tcaacagtat ccaccaggtg gagctgatcc actatgtgct gggcccccag
     2281 catctgcggg atgtcaccac cgccaacctg gagcgcttca tgcgccgctt caatgagctg
     2341 cagtactggg tggccaccga gctgtgtctc tgccccgtgc ccggcccccg ggcccagctg
     2401 ctcaggaagt tcattaagct ggcggcccac ctcaaggagc agaagaatct caattccttc
     2461 tttgccgtca tgtttggcct cagcaactcg gccatcagcc gcctagccca cacctgggag
     2521 cggctgcccc acaaagtccg gaagctgtac tccgccctcg agaggctgct ggatccctca
     2581 tggaaccacc gggtataccg actggccctc gccaagctct cccctcctgt catccccttc
     2641 atgccccttc ttctcaaaga catggccgcc attcatgagg gaaaccacac actagtggag
     2701 aatctcatca actttgagaa gatgagaatg atggccagag ccgcgcggat gctgcaccac
     2761 tgccgaagcc acaaccctgt gcctctctca ccactcagaa gccgagtttc ccacctccac
     2821 gaggacagcc aggtggcgag gatttccaca tgctcggagc agtccctgag cacccggagt
     2881 ccagccagca cctgggctta tgtccagcag ctgaaggtca ttgacaacca gcgggaactc
     2941 tcccgcctct cccgagagct ggagccagct agcgagctca tggacggcgg cgtgcagctc
     3001 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     3061 cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     3121 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     3181 ggtggcagcg gtggcatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     3241 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     3301 ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc
     3361 gtgccctggc ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt cgcccgctac
     3421 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     3481 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     3541 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     3601 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc
     3661 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat cgagccgaat
     3721 tccctcgagg ttaacgcggc gcgcctctaa tctagagggc cctattctat agtgtcacct
     3781 aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     3841 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     3901 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     3961 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     4021 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     4081 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4141 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4201 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4261 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     4321 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4381 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     4441 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4501 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     4561 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     4621 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     4681 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4741 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     4801 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     4861 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg
     4921 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     4981 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     5041 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     5101 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     5161 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     5221 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     5281 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     5341 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     5401 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     5461 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     5521 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     5581 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     5641 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     5701 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     5761 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     5821 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     5881 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5941 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6001 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     6061 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     6121 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     6181 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     6241 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     6301 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6361 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6421 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6481 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6541 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6601 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6661 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6721 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6781 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6841 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     6901 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6961 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7021 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7081 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7141 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7201 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7261 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7321 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7381 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7441 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7501 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7561 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7621 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7681 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7741 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7801 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7861 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7921 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7981 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     8041 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     8101 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     8161 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     8221 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
     8281 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
     8341 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
     8401 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
     8461 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     8521 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     8581 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     8641 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     8701 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     8761 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     8821 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     8881 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     8941 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     9001 tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac ccactgctta
     9061 ctggcttatc gaaattaata cgactcacta tagggagacc c
//
