LOCUS       sequence-335924-       14610 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of constitutively active STAT5A..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14610)
  AUTHORS   Richter WF, Shah RN, Ruthenburg AJ
  TITLE     Non-canonical H3K79me2-dependent pathways promote the survival of 
            MLL-rearranged leukemia.
  JOURNAL   Elife. 2021 Jul 15;10. pii: 64960. doi: 10.7554/eLife.64960.
  PUBMED    34263728
REFERENCE   2  (bases 1 to 14610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-07-15"; volume: "10. pii: 64960. doi"; pages: " 
            10.7554/eLife.64960"
COMMENT     Sequence Label: pBR-Lent-STAT5A-CA
FEATURES             Location/Qualifiers
     source          1..14610
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    2722..2740
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2757..2775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2793..2811
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2829..2847
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2864..2882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2900..2918
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2930..2968
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2965..2989
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3001..3024
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        5428..6639
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6476..6495
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             6658..7365
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     misc_feature    7388..7960
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7555..7572)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7782..7801
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7963..8565
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     8462..8482
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8581..9169
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8634..8654)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9052..9063)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9376..9609
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9710..9727)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9716..9940
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9986..10414
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10073..10092)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10283..10304
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10423..10443)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10428..10757
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10608..10743
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10670..10689
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    11965..12086
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12135..12151)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12135..12151)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12148..12170)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12159..12175
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12183..12189,12190..12207,12208..12213))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12228..12249
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12366..12383)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12537..13125)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12617..12636)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13296..14087,14088..14156))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13919..13938
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14157..14261)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    14309..14443
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gacgagttta ctccctatca gtgatagaga acgatgtcga gtttactccc
     2761 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
     2821 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga
     2881 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt
     2941 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg caccggtatg
     3001 gactataagg atgacgacga caagggttcc ggtgccatgg cgggctggat ccaggcccag
     3061 cagctgcagg gagacgcgct gcgccagatg caggtgctgt acggccagca cttccccatc
     3121 gaggtccggc actacttggc ccagtggatt gagagccagc catgggatgc cattgacttg
     3181 gacaatcccc aggacagagc ccaagccacc cagctcctgg agggcctggt gcaggagctg
     3241 cagaagaagg cggagcacca ggtgggggaa gatgggtttt tactgaagat caagctgggg
     3301 cactacgcca cgcagctcca gaaaacatat gaccgctgcc ccctggagct ggtccgctgc
     3361 atccggcaca ttctgtacaa tgaacagagg ctggtccgag aagccaacaa ttgcagctct
     3421 ccggctggga tcctggttga cgccatgtcc cagaagcacc ttcagatcaa ccagacattt
     3481 gaggagctgc gactggtcac gcaggacaca gagaatgagc tgaagaaact gcagcagact
     3541 caggagtact tcatcatcca gtaccaggag agcctgagga tccaagctca gtttgcccag
     3601 ctggcccagc tgagccccca ggagcgtctg agccgggaga cggccctcca gcagaagcag
     3661 gtgtctctgg aggcctggtt gcagcgtgag gcacagacac tgcagcagta ccgcgtggag
     3721 ctggccgaga agcaccagaa gaccctgcag ctgctgcgga agcagcagac catcatcctg
     3781 gatgacgagc tgatccagtg gaagcggcgg cagcagctgg ccgggaacgg cgggcccccc
     3841 gagggcagcc tggacgtgct acagtcctgg tgtgagaagt tggccgagat catctggcag
     3901 aaccggcagc agatccgcag ggctgagcgc ctctgccagc agctgcccat ccccggccca
     3961 gtggaggaga tgctggccga ggtcaacgcc accatcacgg acattatctc agccctggtg
     4021 accagcacat tcatcattga gaagcagcct cctcaggtcc tgaagaccca gaccaagttt
     4081 gcagccaccg tacgcctgct ggtgggcggg aagctgaacg tgcacatgaa tcccccccag
     4141 gtgaaggcca ccatcatcag tgagcagcag gccaagtctc tgcttaaaaa tgagaacacc
     4201 cgcaacgagt gcagtggtga gatcctgaac aactgctgcg tgatggagta ccaccaagcc
     4261 acgggcaccc tcagtgccca cttcaggaac atgtcactga agaggatcaa gcgtgctgac
     4321 cggcggggtg cagagtccgt gacagaggag aagttcacag tcctgtttga gtctcagttc
     4381 agtgttggca gcaatgagct tgtgttccag gtgaagactc tgtccctacc tgtggttgtc
     4441 atcgtccacg gcagccagga ccacaatgcc acggctactg tgctgtggga caatgccttt
     4501 gctgagccgg gcagggtgcc atttgccgtg cctgacaaag tgctgtggcc gcagctgtgt
     4561 gaggcgctca acatgaaatt caaggccgaa gtgcagagca accggggcct gaccaaggag
     4621 aacctcgtgt tcctggcgca gaaactgttc aacaacagca gcagccacct ggaggactac
     4681 agtggcctgt ccgtgtcctg gtcccagttc aacagggaga acttgccggg ctggaactac
     4741 accttctggc agtggtttga cggggtgatg gaggtgttga agaagcacca caagccccac
     4801 tggaatgatg gggccatcct aggttttgtg aataagcaac aggcccacga cctgctcatc
     4861 aacaagcccg acgggacctt cttgttgcgc tttagtgact cagaaatcgg gggcatcacc
     4921 atcgcctgga agtttgactc cccggaacgc aacctgtgga acctgaaacc attcaccacg
     4981 cgggatttct ccatcaggtc cctggctgac cggctggggg acctgagcta tctcatctat
     5041 gtgtttcctg accgccccaa ggatgaggtc ttctccaagt actacactcc tgtgctggct
     5101 aaagctgttg atggatatgt gaaaccacag atcaagcaag tggtccctga gtttgtgaat
     5161 gcattcgcag atgctggggg cagcagcgcc acgtacatgg accaggcccc ctccccagct
     5221 gtgtgccccc aggctcccta taacatgtac ccacagaacc ctgaccatgt actcgatcag
     5281 gatggagaat tcgacctgga tgagaccatg gatgtggcca ggcacgtgga ggaactctta
     5341 cgccgaccaa tggacagtct tgactcccgc ctctcgcccc ctgccggtct tttcacctct
     5401 gccagaggct ccctctcatg aacgcgtggc ctccgcgccg ggttttggcg cctcccgcgg
     5461 gcgcccccct cctcacggcg agcgctgcca cgtcagacga agggcgcagc gagcgtcctg
     5521 atccttccgc ccggacgctc aggacagcgg cccgctgctc ataagactcg gccttagaac
     5581 cccagtatca gcagaaggac attttaggac gggacttggg tgactctagg gcactggttt
     5641 tctttccaga gagcggaaca ggcgaggaaa agtagtccct tctcggcgat tctgcggagg
     5701 gatctccgtg gggcggtgaa cgccgatgat tatataagga cgcgccgggt gtggcacagc
     5761 tagttccgtc gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc
     5821 acttggtgag tagcgggctg ctgggctggc cggggctttc gtggccgccg ggccgctcgg
     5881 tgggacggaa gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt
     5941 gccctgaact gggggttggg gggagcgcag caaaatggcg gctgttcccg agtcttgaat
     6001 ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg catggtgggc
     6061 ggcaagaacc caaggtcttg aggccttcgc taatgcggga aagctcttat tcgggtgaga
     6121 tgggctgggg caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg
     6181 tttgtcgtct gttgcggggg cggcagttat ggcggtgccg ttgggcagtg cacccgtacc
     6241 tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca
     6301 gggtggggcc acctgccggt aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt
     6361 tcgggcctag ggtaggctct cctgaatcga caggcgccgg acctctggtg aggggaggga
     6421 taagtgaggc gtcagtttct ttggtcggtt ttatgtacct atcttcttaa gtagctgaag
     6481 ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca
     6541 ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc agtgttagac tagtaaattg
     6601 tccgctaaat tctggccgtt tttggctttt ttgttagacg ctagcggatc cgccaccatg
     6661 tctaggctgg acaagagcaa agtcataaac ggagctctgg aattactcaa tggtgtcggt
     6721 atcgaaggcc tgacgacaag gaaactcgct caaaagctgg gagttgagca gcctaccctg
     6781 tactggcacg tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga gatgctggac
     6841 aggcatcata cccacttctg ccccctggaa ggcgagtcat ggcaagactt tctgcggaac
     6901 aacgccaagt cataccgctg tgctctcctc tcacatcgcg acggggctaa agtgcatctc
     6961 ggcacccgcc caacagagaa acagtacgaa accctggaaa atcagctcgc gttcctgtgt
     7021 cagcaaggct tctccctgga gaacgcactg tacgctctgt ccgccgtggg ccactttaca
     7081 ctgggctgcg tattggagga acaggagcat caagtagcaa aagaggaaag agagacacct
     7141 accaccgatt ctatgccccc acttctgaga caagcaattg agctgttcga ccggcaggga
     7201 gccgaacctg ccttcctttt cggcctggaa ctaatcatat gtggcctgga gaaacagcta
     7261 aagtgcgaaa gcggcgggcc gaccgacgcc cttgacgatt ttgacttaga catgctccca
     7321 gccgatgccc ttgacgattt tgaccttgac atgctccccg ggtaaggatc cgcggccgca
     7381 aattccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
     7441 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     7501 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     7561 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     7621 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     7681 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     7741 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     7801 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     7861 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     7921 accacgggga cgtggttttc ctttgaaaaa cacgataata ccatggccac cgagtacaag
     7981 cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc
     8041 gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca catcgagcgg
     8101 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     8161 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     8221 gcggtgttcg ccgagatcgg ctcgcgcatg gccgagttga gcggttcccg gctggccgcg
     8281 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     8341 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     8401 cccggagtgg aggcggccga gcgcgctggg gtgcccgcct tcctggagac ctccgcgccc
     8461 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     8521 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgagttcg cgtctggaac
     8581 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     8641 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     8701 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     8761 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     8821 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     8881 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     8941 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     9001 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     9061 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     9121 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat
     9181 tctgcagtcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     9241 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     9301 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     9361 aagaaaagag gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     9421 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     9481 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     9541 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     9601 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     9661 gaatatcaga gagtgagagg ccttgacatt gtttaaaccc gctgatcagc ctcgactgtg
     9721 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     9781 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     9841 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     9901 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     9961 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
    10021 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
    10081 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
    10141 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
    10201 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
    10261 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
    10321 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
    10381 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
    10441 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
    10501 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
    10561 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
    10621 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
    10681 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
    10741 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
    10801 acgtgatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt
    10861 tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct
    10921 tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca
    10981 aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg
    11041 acattgggga attaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt
    11101 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
    11161 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
    11221 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
    11281 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
    11341 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
    11401 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
    11461 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
    11521 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
    11581 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
    11641 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
    11701 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
    11761 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
    11821 agggcaaagg aatagcacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
    11881 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
    11941 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
    12001 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    12061 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
    12121 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
    12181 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
    12241 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
    12301 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
    12361 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    12421 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    12481 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    12541 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    12601 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    12661 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    12721 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    12781 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    12841 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    12901 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    12961 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    13021 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    13081 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    13141 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    13201 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    13261 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    13321 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    13381 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    13441 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    13501 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    13561 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    13621 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    13681 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    13741 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    13801 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    13861 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    13921 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    13981 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    14041 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    14101 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    14161 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    14221 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    14281 ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa
    14341 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    14401 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
    14461 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
    14521 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
    14581 ttgttaaata tgtactacaa acttagtagt
//
