LOCUS       sequence-33593-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CIB1 fused to plasma membrane-localized EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Kennedy MJ, Hughes RM, Peteya LA, Schwartz JW, Ehlers MD, Tucker CL
  TITLE     Rapid blue-light-mediated induction of protein interactions in 
            living cells.
  JOURNAL   Nat Methods. 2010 Dec;7(12):973-5. doi: 10.1038/nmeth.1524
  PUBMED    21037589
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2010-12"; volume: "7"; issue: 
            "12"; pages: "973-5"
COMMENT     Sequence Label: pCIB1(deltaNLS)-pmGFP
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgcccttgct caccatggtg gcgaccggta ctcctaaatt gccatagaga ttctgcacat
       61 gagagtccca catcgaagga gcttcgccat tgttgaagca tgacaatgga tcagaactgg
      121 tattcacttg ctgcattgga ttgacatgaa ggtaaccgga gttaaccatc tcactagaat
      181 aaccagagtg aaccatctca tgagaataac cggaaagaac catttcagga gatggcacca
      241 cagtcattgg agttgaggca acctctttgg caaaaatgtc atccatatca aaatccggcc
      301 ttggattcac aattgctagt ttcatcgata agaactcgat ttgtctctga agagactgaa
      361 catagttaat gatttcatca agcatccctg ctttgcctgt gatcttgtcg catccaggaa
      421 ccaaatcttg tagaaacttc attctctcac tgatcttttc tcttctaact cgttctgcta
      481 tgctgtgact atcagtggct tggcctcgtc gtgcacgaac atgaatataa tccgttttct
      541 ccaattcctt cgtcacttta gatgaatcat tagagaaatt gttctcttct ttcttggctt
      601 tgtgtttcat cttcttgatg ctttttgtgc tcccattgtt ttgctctgtt attttcgact
      661 tctcttcttc tccttcttct actaggtcat ctctgttcat cgtcatcttc ttcgccgcct
      721 cattacaatc cttagtctct gtatcaaact tcgctgcctt gaaatttcca gtcccaagcg
      781 tcgtttccgg cgaaattgag agtcttgaat caccttccac cgtcgtctca ccgtacatca
      841 tcggaagatt caaaccggca gtcgaaagat agctgtccat ctcgccgccg gtaat
//
