LOCUS       sequence-335943-       10865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of RUNX1 P98R mutant protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, 
            Hutter S, Haferlach T, Ideker T, Mali P, Carter H
  TITLE     Interface-guided phenotyping of coding variants in the transcription
            factor RUNX1.
  JOURNAL   Cell Rep. 2024 Jul 4;43(7):114436. doi: 
            10.1016/j.celrep.2024.114436.
  PUBMED    38968069
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-4"; volume: "43(7):114436. doi"; pages: " 
            10.1016/j.celrep.2024.114436"
COMMENT     Sequence Label: EF1A_Hygro_P2A_RUNX1_P98R_Barcode
FEATURES             Location/Qualifiers
     source          1..10865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(40..60)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        45..374
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      225..360
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        422..469
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             488..862
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    992..1113
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1029..1048)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1083..1102
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1162..1178)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1162..1178)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1175..1197)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1186..1202
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1210..1216,1217..1234,1235..1240))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1255..1276
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1393..1410)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1564..2152)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1644..1663)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2323..3114,3115..3183))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2946..2965
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3184..3288)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3363..3382)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3554..3933
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3935..4133
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4088..4108
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4151..4331
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4378..4503
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4996..5229
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5414..5458
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5756..5873
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6009..7187
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6240..7178
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7135..7155
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     7204..7223
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7204..7222
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7286..8308
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7286..8308
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8318..8374
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(9928..9961)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             9986..10166
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10192..10209)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10198..10422
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(10468..10865,1..31)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10555..10574)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10765..10786
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
       61 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
      121 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
      181 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
      241 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
      301 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
      361 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
      421 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
      481 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
      541 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
      601 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
      661 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
      721 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
      781 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
      841 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
      901 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
      961 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     1021 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     1081 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     1141 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     1201 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     1261 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     1321 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     1381 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     1441 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1501 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1561 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1621 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1681 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1741 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1801 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1861 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1921 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1981 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     2041 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2101 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2161 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     2221 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     2281 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     2341 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     2401 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     2461 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     2521 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     2581 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     2641 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     2701 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     2761 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     2821 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     2881 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     2941 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     3001 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     3061 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     3121 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     3181 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     3241 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     3301 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
     3361 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
     3421 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
     3481 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
     3541 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     3601 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     3661 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     3721 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     3781 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     3841 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     3901 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     3961 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     4021 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     4081 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt
     4141 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     4201 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     4261 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     4321 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     4381 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     4441 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     4501 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     4561 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     4621 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     4681 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     4741 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     4801 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     4861 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     4921 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     4981 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     5041 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     5101 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     5161 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     5221 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     5281 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     5341 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     5401 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     5461 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     5521 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     5581 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     5641 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     5701 cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta
     5761 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     5821 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     5881 attacaggga cagcagagat ccagtttggt taattaatcg ataagctttg caaagatgga
     5941 taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag
     6001 tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     6061 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
     6121 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     6181 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     6241 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
     6301 cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa
     6361 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg
     6421 aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc
     6481 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt
     6541 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt
     6601 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg
     6661 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc
     6721 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt
     6781 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa
     6841 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg
     6901 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc
     6961 acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt
     7021 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact
     7081 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc
     7141 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga attagcttgg
     7201 tactaatacg actcactata gggagaccca agctggctag gtaagcttga tatcgaattc
     7261 ctgcagcccg ggggatcccg ccaccatgaa aaagcctgaa ctcaccgcga cgtctgtcga
     7321 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga
     7381 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
     7441 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
     7501 cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc
     7561 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
     7621 gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg
     7681 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
     7741 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
     7801 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
     7861 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
     7921 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
     7981 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
     8041 gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga
     8101 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
     8161 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
     8221 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
     8281 ccccagcact cgtccgaggg caaaggaagg atccggcgca acaaacttct ctctgctgaa
     8341 acaagccgga gatgtcgaag agaatcctgg accgatgcgt atccccgtag atgccagcac
     8401 gagccgccgc ttcacgccgc cttccaccgc gctgagccca ggcaagatga gcgaggcgtt
     8461 gccgctgggc gccccggacg ccggcgctgc cctggccggc aagctgagga gcggcgaccg
     8521 cagcatggtg gaggtgctgg ccgaccaccc gggcgagctg gtgcgcaccg acagccccaa
     8581 cttcctctgc tccgtgctgc ctacgcactg gcgctgcaac aagaccctgc ccatcgcttt
     8641 caaggtggtg gccctagggg atgttcgaga tggcactctg gtcactgtga tggctggcaa
     8701 tgatgaaaac tactcggctg agctgagaaa tgctaccgca gccatgaaga accaggttgc
     8761 aagatttaat gacctcaggt ttgtcggtcg aagtggaaga gggaaaagct tcactctgac
     8821 catcactgtc ttcacaaacc caccgcaagt cgccacctac cacagagcca tcaaaatcac
     8881 agtggatggg ccccgagaac ctcgaagaca tcggcagaaa ctagatgatc agaccaagcc
     8941 cgggagcttg tccttttccg agcggctcag tgaactggag cagctgcggc gcacagccat
     9001 gagggtcagc ccacaccacc cagcccccac gcccaaccct cgtgcctccc tgaaccactc
     9061 cactgccttt aaccctcagc ctcagagtca gatgcaggat acaaggcaga tccaaccatc
     9121 cccaccgtgg tcctacgatc agtcctacca atacctggga tccattgcct ctccttctgt
     9181 gcacccagca acgcccattt cacctggacg tgccagcggc atgacaaccc tctctgcaga
     9241 actttccagt cgactctcaa cggcacccga cctgacagcg ttcagcgacc cgcgccagtt
     9301 ccccgcgctg ccctccatct ccgacccccg catgcactat ccaggcgcct tcacctactc
     9361 cccgacgccg gtcacctcgg gcatcggcat cggcatgtcg gccatgggct cggccacgcg
     9421 ctaccacacc tacctgccgc cgccctaccc cggctcgtcg caagcgcagg gaggcccgtt
     9481 ccaagccagc tcgccctcct accacctgta ctacggcgcc tcggccggct cctaccagtt
     9541 ctccatggtg ggcggcgagc gctcgccgcc gcgcatcctg ccgccctgca ccaacgcctc
     9601 caccggctcc gcgctgctca accccagcct cccgaaccag agcgacgtgg tggaggccga
     9661 gggcagccac agcaactccc ccaccaacat ggcgccctcc gcgcgcctgg aggaggccgt
     9721 gtggaggccc tactgactag ctaagttaga actatttcct ggctgttacg cgtcgacgta
     9781 tgtactactg acgggcttgt taaacagtga accgcccgcc ccacgacccg cagcgcccga
     9841 ccgaaaggag cgcacgaccc catgcatcga acaaaagaaa aggggggact ggaagggcta
     9901 attcactccc aacgaagaca agatatcata acttcgtata gcatacatta tacgaagtta
     9961 tcgtctagct ggtccggact gtactgggtc tctctggtta gaccagatct gagcctggga
    10021 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
    10081 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
    10141 ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg
    10201 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
    10261 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
    10321 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
    10381 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
    10441 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
    10501 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
    10561 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
    10621 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
    10681 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
    10741 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
    10801 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
    10861 aaaat
//
