LOCUS       sequence-33595-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Mosimann C, Kaufman CK, Li P, Pugach EK, Tamplin OJ, Zon LI
  TITLE     Ubiquitous transgene expression and Cre-based recombination driven 
            by the ubiquitin promoter in zebrafish.
  JOURNAL   Development. 2011 Jan . 138(1):169-77.
  PUBMED    21138979
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2011 Jan . 138(1):169-77."
COMMENT     Sequence Label: pENTR5'_ubi:loxP-EGFP-loxP
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    29..62
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cgcccttgct caccatggtg gcgaccggat aacttcgtat aatgtatgct atacgaagtt
       61 ataagcttgg atccctgtaa acaaattcaa agtaagatta gcaatcacac atttccattt
      121 aaaaaatact acaatgttct agccaataat gtatgttttg cacttctccc atgttaaacg
      181 agatagttca cccgaaaatg aaattttact ttgcatttgg tcacccttaa atgttacaaa
      241 cctttaaaat aatagaaaac tattgactat acttgtagag aaaaataaat actatccaaa
      301 acaatggctg acagtggact atagaatgca ttttacctgc agcaaaattg tccaaatgat
      361 cctatatgaa aatttagggt cttacctagt gaaatgatag ctcattttgc acaagctctg
      421 agatttggct ttgtgcatca tgaaatctaa ttatgtcatg catttccaaa gcattggcca
      481 ttttaaacaa actgcacatc tgtgcagttt acattgtttt aaaataaaaa ataaaaaatt
      541 aagaaataaa gaataataat gttccacaac attacattag gtagcagatt ggcagagaac
      601 caaagggtgg ggccagtaag gaaggacttc tacacagtaa ggaaacgctg aaattcaact
      661 taatcactca gtcatttaaa aataaatact tttatttttt ttatttttaa aggtctaatg
      721 gtttcattag ataagactgt tattccttga ctgggatcat ttagaaccat ttgaagccac
      781 atattgcatt ttggaatttt aaactaacaa gctccacaat atagagaaaa aaatcttgaa
      841 actttttcaa aaacaatttc ttcattaatg atgaaaaaaa gggatca
//
