LOCUS       sequence-335969-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This gene was cloned into the gateway middle entry vector. It can be
            used for generating antisense riboprobe or gene expression using a 
            gateway system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Silic MR, Murata SH, Park SJ, Zhang G
  TITLE     Evolution of inwardly rectifying potassium channels and their gene 
            expression in zebrafish embryos.
  JOURNAL   Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.425.
  PUBMED    34558132
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2021 Sep 23. doi: 10.1002/dvdy.425."
COMMENT     Sequence Label: pDONR221-kcnj9
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct
       61 ttcttataat gccaactttg tacaagaaag ctgggtctta agcctcagac tctgattggt
      121 cagtggcgac actgccgttc tgctctccta caaccgactg gtcctgggta tttgtaactc
      181 cacccacagt aaatgttggc ccttgttcct ccctgacaga ttctgaatca gtggattttc
      241 ctgtttgctc agtcagatta ggcccctccc atttctcttc atcaagccga ctggagatcg
      301 accaatagag atgagcatcc aaacgggcgg cagccaatgc cagctctctt gcagagcagg
      361 agggtgtgtc cacctcaaat gtgtggtgaa atgccccata atccacgtca aagaatccct
      421 cggccagaca catcatggga ctaaacctgt gaccccacat gacctcctca gccaggtaag
      481 agctccgtgc ctgacaggtc atacctgttg cttccaccat cccctccaag atgacaacaa
      541 tctcgaagtc ctctttctcc agctgatttt gtgacatgtc ccagaacgga gagcgtacat
      601 cgatttcatg agagatcacc agaggagaca ccagaaacag acgatcatct cccgtctcga
      661 agcccacact gatatcagtt tgatctagcg gaataaactc accctcttgt gtctgtttgg
      721 atttgataag tttggctctc atgttggcgc ccacaatgtg ggaggacctc aagtct
//
