LOCUS       sequence-335979-       10865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of RUNX1 L94P mutant protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, 
            Hutter S, Haferlach T, Ideker T, Mali P, Carter H
  TITLE     Interface-guided phenotyping of coding variants in the transcription
            factor RUNX1.
  JOURNAL   Cell Rep. 2024 Jul 4;43(7):114436. doi: 
            10.1016/j.celrep.2024.114436.
  PUBMED    38968069
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-4"; volume: "43(7):114436. doi"; pages: " 
            10.1016/j.celrep.2024.114436"
COMMENT     Sequence Label: EF1A_Hygro_P2A_RUNX1_L94P_Barcode
FEATURES             Location/Qualifiers
     source          1..10865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(70..90)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        75..404
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      255..390
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        452..499
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             518..892
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1022..1143
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1059..1078)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1113..1132
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1192..1208)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1192..1208)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1205..1227)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1216..1232
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1240..1246,1247..1264,1265..1270))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1285..1306
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1423..1440)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1594..2182)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1674..1693)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2353..3144,3145..3213))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2976..2995
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3214..3318)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3393..3412)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3584..3963
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3965..4163
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4118..4138
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4181..4361
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4408..4533
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5026..5259
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5444..5488
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5786..5903
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6039..7217
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6270..7208
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7165..7185
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     7234..7253
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7234..7252
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7316..8338
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7316..8338
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8348..8404
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(9958..9991)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             10016..10196
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10222..10239)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10228..10452
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(10498..10865,1..61)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10585..10604)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10795..10816
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
       61 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
      121 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
      181 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
      241 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
      301 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
      361 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
      421 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat
      481 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt
      541 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
      601 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
      661 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
      721 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
      781 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
      841 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct
      901 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
      961 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     1021 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     1081 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     1141 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     1201 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     1261 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     1321 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     1381 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     1441 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     1501 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     1561 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1621 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1681 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1741 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1801 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1861 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1921 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1981 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     2041 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     2101 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     2161 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     2221 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     2281 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     2341 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     2401 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     2461 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     2521 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     2581 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     2641 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     2701 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     2761 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     2821 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     2881 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     2941 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     3001 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     3061 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     3121 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     3181 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     3241 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     3301 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     3361 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     3421 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     3481 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     3541 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
     3601 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     3661 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     3721 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     3781 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     3841 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     3901 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     3961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     4021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     4081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     4141 cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca
     4201 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     4261 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     4321 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     4381 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     4441 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     4501 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     4561 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     4621 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     4681 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     4741 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     4801 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     4861 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     4921 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     4981 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     5041 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     5101 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     5161 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     5221 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     5281 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     5341 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     5401 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     5461 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     5521 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     5581 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     5641 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     5701 agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca
     5761 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     5821 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     5881 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt
     5941 taattaatcg ataagctttg caaagatgga taaagtttta aacagagagg aatctttgca
     6001 gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg
     6061 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     6121 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     6181 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     6241 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     6301 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     6361 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     6421 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     6481 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     6541 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     6601 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     6661 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     6721 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg
     6781 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     6841 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     6901 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     6961 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     7021 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     7081 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     7141 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     7201 catttcaggt gtcgtgagga attagcttgg tactaatacg actcactata gggagaccca
     7261 agctggctag gtaagcttga tatcgaattc ctgcagcccg ggggatcccg ccaccatgaa
     7321 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt
     7381 ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg
     7441 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta
     7501 tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga
     7561 attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga
     7621 cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat
     7681 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg
     7741 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg
     7801 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat
     7861 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa
     7921 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt
     7981 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat
     8041 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct
     8101 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
     8161 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg
     8221 gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt
     8281 agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaagg
     8341 atccggcgca acaaacttct ctctgctgaa acaagccgga gatgtcgaag agaatcctgg
     8401 accgatgcgt atccccgtag atgccagcac gagccgccgc ttcacgccgc cttccaccgc
     8461 gctgagccca ggcaagatga gcgaggcgtt gccgctgggc gccccggacg ccggcgctgc
     8521 cctggccggc aagctgagga gcggcgaccg cagcatggtg gaggtgctgg ccgaccaccc
     8581 gggcgagctg gtgcgcaccg acagccccaa cttcctctgc tccgtgctgc ctacgcactg
     8641 gcgctgcaac aagaccctgc ccatcgcttt caaggtggtg gccccagggg atgttccaga
     8701 tggcactctg gtcactgtga tggctggcaa tgatgaaaac tactcggctg agctgagaaa
     8761 tgctaccgca gccatgaaga accaggttgc aagatttaat gacctcaggt ttgtcggtcg
     8821 aagtggaaga gggaaaagct tcactctgac catcactgtc ttcacaaacc caccgcaagt
     8881 cgccacctac cacagagcca tcaaaatcac agtggatggg ccccgagaac ctcgaagaca
     8941 tcggcagaaa ctagatgatc agaccaagcc cgggagcttg tccttttccg agcggctcag
     9001 tgaactggag cagctgcggc gcacagccat gagggtcagc ccacaccacc cagcccccac
     9061 gcccaaccct cgtgcctccc tgaaccactc cactgccttt aaccctcagc ctcagagtca
     9121 gatgcaggat acaaggcaga tccaaccatc cccaccgtgg tcctacgatc agtcctacca
     9181 atacctggga tccattgcct ctccttctgt gcacccagca acgcccattt cacctggacg
     9241 tgccagcggc atgacaaccc tctctgcaga actttccagt cgactctcaa cggcacccga
     9301 cctgacagcg ttcagcgacc cgcgccagtt ccccgcgctg ccctccatct ccgacccccg
     9361 catgcactat ccaggcgcct tcacctactc cccgacgccg gtcacctcgg gcatcggcat
     9421 cggcatgtcg gccatgggct cggccacgcg ctaccacacc tacctgccgc cgccctaccc
     9481 cggctcgtcg caagcgcagg gaggcccgtt ccaagccagc tcgccctcct accacctgta
     9541 ctacggcgcc tcggccggct cctaccagtt ctccatggtg ggcggcgagc gctcgccgcc
     9601 gcgcatcctg ccgccctgca ccaacgcctc caccggctcc gcgctgctca accccagcct
     9661 cccgaaccag agcgacgtgg tggaggccga gggcagccac agcaactccc ccaccaacat
     9721 ggcgccctcc gcgcgcctgg aggaggccgt gtggaggccc tactgactag ctaagttaga
     9781 actatttcct ggctgttacg cgtgtagggg catgctactg acgggcttgt taaacagtga
     9841 accgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcga
     9901 acaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcata
     9961 acttcgtata gcatacatta tacgaagtta tcgtctagct ggtccggact gtactgggtc
    10021 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
    10081 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
    10141 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc
    10201 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
    10261 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
    10321 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
    10381 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
    10441 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
    10501 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
    10561 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
    10621 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
    10681 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
    10741 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
    10801 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
    10861 ggatt
//
