LOCUS       sequence-33598-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, 
            Eichtinger M, Jiang T, Foley JE, Winfrey RJ, Townsend JA, 
            Unger-Wallace E, Sander JD, Muller-Lerch F, Fu F, Pearlberg J, 
            Goebel C, Dassie JP, Pruett-Miller SM, Porteus MH, Sgroi DC, Iafrate
            AJ, Dobbs D, McCray PB, Cathomen T, Voytas DF, Joung JK
  TITLE     Rapid ""open-source"" engineering of customized zinc-finger 
            nucleases for highly efficient gene modification.
  JOURNAL   Mol Cell. 2008 Jul 25. 31(2):294-301.
  PUBMED    18657511
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2008 Jul 25. 31(2):294-301."
COMMENT     Sequence Label: pitrm1_L (OZ515)
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..65
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     rep_origin      467..847
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(554..573)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     764..785
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tgcaaaggcc ctgagaaaaa atccaagctt tggcggccgc tgactacaaa gacgatgatg
       61 acaaatctag acccggggag cgccccttcc agtgtcgcat ttgcatgcgg aacttttcga
      121 ccaagcagag gcttcacatc catacccgta ctcataccgg tgaaaaaccg tttcagtgtc
      181 ggatctgtat gcgaaatttc tcccagagcg ggacgttggt ccgccatcta cgtacgcaca
      241 ccggcgagaa gccattccaa tgccgaatat gcatgcgcaa cttcagtcag aggagcgacc
      301 tgcaccggca cctaaaaacc cacctgaggg gatcctctgc acagtagtaa ctagagacta
      361 gaaaaaggcc gacaagtccc gctccgctga agatcctggc gtaatagcga agaggcccgc
      421 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
      481 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
      541 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
      601 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
      661 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
      721 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
      781 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
      841 atttcggcct atnggttaa
//
