LOCUS       sequence-335982-       10859 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of STK38L (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10859)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-STK38L_IDG-K
FEATURES             Location/Qualifiers
     source          1..10859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     45..64
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             133..732
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(133..152)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     629..649
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             870..1103
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1175..1296
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1212..1231)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1266..1285
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1336..1471
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1491..1510)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1492..1510)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1520..1537)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1520..1536)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1529..1551)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1678..2133
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1765..1784)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1975..1996
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2159..2263
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2264..2332,2333..3124)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3295..3883
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3784..3803
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4037..4054
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4171..4192
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4207..4212,4213..4230,4231..4237)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4245..4261
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4250..4272
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4269..4285
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4269..4285
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4304..4324
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4306..4324
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4352..4578
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4579..4759
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4806..4931
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5424..5657
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5842..5886
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6153..6269
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6303..6323
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6323..7534
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7371..7390
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(7538..7558)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      7559..7568
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7571..8341
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             8348..8389
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    8390..8414
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9816..9840)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9870..10458
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9923..9943)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10341..10352)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        join(10472..10859,1..112)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10507..10530)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
       61 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag
      121 cccaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
      181 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
      241 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
      301 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
      361 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
      421 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
      481 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
      541 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
      601 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
      661 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
      721 cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc
      781 catgcatctc gagggcccgg tacctttaag accaatgact tacaaggcag ctgtagatct
      841 tagccacttt ttaaaagaaa aggggggact ggaagggcta gctcactccc aacgaagaca
      901 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
      961 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     1021 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     1081 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     1141 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     1201 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     1261 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     1321 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     1381 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     1441 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     1501 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     1561 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     1621 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     1681 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     1741 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     1801 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     1861 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     1921 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     1981 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     2041 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     2101 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     2161 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     2221 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     2281 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     2341 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     2401 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     2461 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     2521 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     2581 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     2641 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     2701 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     2761 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     2821 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     2881 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     2941 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     3001 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     3061 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     3121 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     3181 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     3241 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3301 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3361 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     3421 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     3481 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     3541 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     3601 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     3661 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     3721 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     3781 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     3841 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     3901 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     3961 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     4021 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     4081 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     4141 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     4201 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     4261 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
     4321 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
     4381 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
     4441 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
     4501 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
     4561 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     4621 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     4681 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     4741 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     4801 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     4861 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     4921 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     4981 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     5041 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     5101 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     5161 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     5221 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     5281 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     5341 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     5401 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     5461 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     5521 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     5581 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     5641 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     5701 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     5761 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     5821 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     5881 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     5941 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     6001 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     6061 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     6121 ttagtgaacg gatctcgacg gtatcggtta acttttaaaa gaaaaggggg gattgggggg
     6181 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     6241 caaaaacaaa ttacaaaatt caaaatttta tcgtccagtg tggtggaatt ctgcagatat
     6301 cacaactttg tatagaaaag ttggcctccg cgccgggttt tggcgcctcc cgcgggcgcc
     6361 cccctcctca cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg tcctgatcct
     6421 tccgcccgga cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag
     6481 tatcagcaga aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt
     6541 ccagagagcg gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct
     6601 ccgtggggcg gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt
     6661 ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg
     6721 gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc cgccgggccg ctcggtggga
     6781 cggaagcgtg tggagagacc gccaagggct gtagtctggg tccgcgagca aggttgccct
     6841 gaactggggg ttggggggag cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag
     6901 acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa
     6961 gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct cttattcggg tgagatgggc
     7021 tggggcacca tctggggacc ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt
     7081 cgtctgttgc gggggcggca gttatggcgg tgccgttggg cagtgcaccc gtacctttgg
     7141 gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata atgcagggtg
     7201 gggccacctg ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc agggttcggg
     7261 cctagggtag gctctcctga atcgacaggc gccggacctc tggtgagggg agggataagt
     7321 gaggcgtcag tttctttggt cggttttatg tacctatctt cttaagtagc tgaagctccg
     7381 gttttgaact atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt aggcaccttt
     7441 tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt tagactagta aattgtccgc
     7501 taaattctgg ccgtttttgg cttttttgtt agacggccaa cttttgtata caaagtgggc
     7561 caccatggga atcccgctgc tgaacgctaa acagattctg ggacagctgg acggcgggag
     7621 cgtggcagtc ctgcctgtgg tcgactccac caatcagtac ctgctggatc gaatcggcga
     7681 gctgaagagt ggggatgctt gcattgcaga atatcagcag gcagggagag gaagcagagg
     7741 gaggaaatgg ttctctcctt ttggagctaa cctgtacctg agtatgtttt ggcgcctgaa
     7801 gcggggacca gcagcaatcg gcctgggccc ggtcatcgga attgtcatgg cagaagcgct
     7861 gcgaaagctg ggagcagaca aggtgcgagt caaatggccc aatgacctgt atctgcagga
     7921 tagaaagctg gcaggcatcc tggtggagct ggccggaata acaggcgatg ctgcacagat
     7981 cgtcattggc gccgggatta acgtggctat gaggcgcgtg gaggaaagcg tggtcaatca
     8041 gggctggatc acactgcagg aagcagggat taacctggac aggaatactc tggccgctat
     8101 gctgatccga gagctgcggg cagccctgga actgttcgag caggaaggcc tggctccata
     8161 tctgtcacgg tgggagaagc tggataactt catcaataga cccgtgaagc tgatcattgg
     8221 ggacaaagag attttcggga ttagccgggg gattgataaa cagggagccc tgctgctgga
     8281 acaggacgga gttatcaaac cctggatggg cggagaaatc agtctgcggt ctgccgaaaa
     8341 ggctagcggc aagcccatcc ccaaccccct gctgggcctg gacagcacca caagtttgta
     8401 caaaaaagca ggctccacca tggcaatgac ggcagggact acaacaacct ttcctatgag
     8461 caaccatacc cgggaaagag tgactgtagc caagctcaca ttggagaatt tttatagcaa
     8521 cctaatttta cagcatgaag agagagaaac caggcagaag aaattagaag tggccatgga
     8581 agaagaagga ttagcagatg aagagaaaaa gttacgtcga tcacaacacg ctcgcaaaga
     8641 aacagagttc ttacggctca aaaggaccag acttggcttg gatgactttg agtctctgaa
     8701 agttatagga agaggagctt ttggagaggt gcggttggtc cagaagaaag atacaggcca
     8761 tatctatgca atgaagatat tgagaaagtc tgatatgctt gaaaaagagc aggtggccca
     8821 tatccgagca gaaagagata ttttggtaga agcagatggt gcctgggtgg tgaagatgtt
     8881 ttacagtttt caggataaga ggaatcttta tctaatcatg gaatttctcc ctggaggtga
     8941 catgatgaca ttgctaatga agaaagacac cttgacagaa gaggaaacac agttctacat
     9001 ttcagagact gttctggcaa tagatgcgat ccaccagttg ggtttcatcc atcgggatat
     9061 taagccagac aaccttttat tggatgccaa gggtcatgta aaattatctg attttggttt
     9121 atgtacggga ttaaagaaag ctcacaggac tgaattttat agaaatctca cacacaaccc
     9181 accaagtgac ttctcatttc agaacatgaa ctcaaagagg aaagcagaaa cttggaagaa
     9241 gaacaggaga caactggcat attccacagt tgggacacca gattacattg ctccagaagt
     9301 attcatgcag actggttaca acaaattgtg tgactggtgg tctttgggag tgattatgta
     9361 tgaaatgcta ataggatatc cacctttctg ctctgaaaca cctcaagaaa catacagaaa
     9421 agtgatgaac tggaaagaaa ctctggtatt tcctccagag gtacctatat ctgagaaagc
     9481 caaggactta attctcagat tttgtattga ttctgaaaac agaattggaa atagtggagt
     9541 agaagaaata aaaggtcatc ccttttttga aggtgtcgac tgggagcaca taagggaaag
     9601 gccagcagca atccctatag aaatcaaaag cattgatgat acttcaaatt ttgatgactt
     9661 ccctgaatct gatattttac aaccagtgcc aaataccaca gaaccggact acaaatccaa
     9721 agactgggtt tttctcaatt atacctataa aaggtttgaa gggttgactc aacgtggctc
     9781 tatccccacc tacatgaaag ctgggaagtt ataggaccca gctttcttgt acaaagtggt
     9841 gatatccagc acagtggcgg ccgctcgaca atcaacctct ggattacaaa atttgtgaaa
     9901 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     9961 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
    10021 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
    10081 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
    10141 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
    10201 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
    10261 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
    10321 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
    10381 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
    10441 tttgggccgc ctccccgcct ggaattctac cgggtagggg aggcgctttt cccaaggcag
    10501 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
    10561 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
    10621 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
    10681 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
    10741 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
    10801 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcgg
//
