LOCUS       sequence-335984-       10778 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of STK40 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10778)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-STK40_IDG-K
FEATURES             Location/Qualifiers
     source          1..10778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        39..143
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(144..212,213..1004)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1175..1763
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1664..1683
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1917..1934
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2051..2072
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2087..2092,2093..2110,2111..2117)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2125..2141
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2130..2152
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2149..2165
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2149..2165
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2184..2204
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2186..2204
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2232..2458
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2459..2639
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2686..2811
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3304..3537
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3722..3766
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4033..4149
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4183..4203
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4203..5414
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5251..5270
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(5418..5438)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      5439..5448
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5451..6221
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             6228..6269
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    6270..6294
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7615..7639)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7669..8257
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7722..7742)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8140..8151)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8271..8770
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8306..8329)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8703..8722
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8791..9390
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8791..8810)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9287..9307
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9528..9761
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9833..9954
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9870..9889)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9924..9943
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9994..10129
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10149..10168)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10150..10168)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10178..10195)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10178..10194)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10187..10209)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10336..10764
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10423..10442)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10633..10654
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
       61 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      121 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
      181 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
      241 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
      301 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
      361 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
      421 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
      481 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
      541 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
      601 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
      661 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
      721 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
      781 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
      841 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
      901 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
      961 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     1021 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     1081 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1141 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1201 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1261 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1321 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1381 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1441 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1501 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     1561 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     1621 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     1681 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     1741 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     1801 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     1861 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     1921 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     1981 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     2041 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     2101 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     2161 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     2221 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     2281 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
     2341 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     2401 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
     2461 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     2521 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     2581 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     2641 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     2701 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     2761 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     2821 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     2881 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     2941 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     3001 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     3061 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     3121 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     3181 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     3241 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     3301 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     3361 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     3421 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     3481 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     3541 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     3601 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     3661 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     3721 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     3781 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     3841 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     3901 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     3961 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
     4021 gtatcggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     4081 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt
     4141 caaaatttta tcgtccagtg tggtggaatt ctgcagatat cacaactttg tatagaaaag
     4201 ttggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc
     4261 tgccacgtca gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac
     4321 agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt
     4381 aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga
     4441 ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg
     4501 atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg
     4561 gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg
     4621 ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc
     4681 gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag
     4741 cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt
     4801 gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc
     4861 ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc
     4921 ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca
     4981 gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg
     5041 tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg
     5101 tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga
     5161 atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt
     5221 cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg
     5281 ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt
     5341 caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg
     5401 cttttttgtt agacggccaa cttttgtata caaagtgggc caccatggga atcccgctgc
     5461 tgaacgctaa acagattctg ggacagctgg acggcgggag cgtggcagtc ctgcctgtgg
     5521 tcgactccac caatcagtac ctgctggatc gaatcggcga gctgaagagt ggggatgctt
     5581 gcattgcaga atatcagcag gcagggagag gaagcagagg gaggaaatgg ttctctcctt
     5641 ttggagctaa cctgtacctg agtatgtttt ggcgcctgaa gcggggacca gcagcaatcg
     5701 gcctgggccc ggtcatcgga attgtcatgg cagaagcgct gcgaaagctg ggagcagaca
     5761 aggtgcgagt caaatggccc aatgacctgt atctgcagga tagaaagctg gcaggcatcc
     5821 tggtggagct ggccggaata acaggcgatg ctgcacagat cgtcattggc gccgggatta
     5881 acgtggctat gaggcgcgtg gaggaaagcg tggtcaatca gggctggatc acactgcagg
     5941 aagcagggat taacctggac aggaatactc tggccgctat gctgatccga gagctgcggg
     6001 cagccctgga actgttcgag caggaaggcc tggctccata tctgtcacgg tgggagaagc
     6061 tggataactt catcaataga cccgtgaagc tgatcattgg ggacaaagag attttcggga
     6121 ttagccgggg gattgataaa cagggagccc tgctgctgga acaggacgga gttatcaaac
     6181 cctggatggg cggagaaatc agtctgcggt ctgccgaaaa ggctagcggc aagcccatcc
     6241 ccaaccccct gctgggcctg gacagcacca caagtttgta caaaaaagca ggctccacca
     6301 tgaagcggag agcatcagac agaggagctg gggaaacgtc ggccagggcc aaggctctgg
     6361 gaagtgggat ttctggaaat aatgcaaaga gagctggacc attcatcctt ggtccccgtc
     6421 tgggcaactc accggtgcca agcatagtgc agtgtttggc gaggaaagat ggcacggatg
     6481 acttctatca gctgaagatc ctgaccctgg aggagagggg ggaccaaggc atagagagcc
     6541 aggaagagcg gcagggcaag atgctgctgc acaccgagta ctcactgctg tctctcctgc
     6601 acacgcagga tggcgtggtg caccaccacg gcctcttcca ggaccgcacc tgtgaaatcg
     6661 ttgaggacac agaatccagc cggatggtta agaagatgaa gaagcgcatc tgcctcgtcc
     6721 tggactgcct ctgtgctcat gacttcagcg ataagaccgc tgacctcatc aacctgcagc
     6781 actacgtcat caaggagaag aggctcagcg agagggagac tgtggtaatc ttctacgacg
     6841 tggtccgcgt ggtggaggcc ctgcaccaga aaaatatcgt gcacagagac ctgaagctgg
     6901 ggaacatggt gctcaacaag aggacacatc ggataaccat caccaacttc tgcctcggga
     6961 agcatctggt gagcgagggg gacctgctga aggaccagag agggagccct gcctacatca
     7021 gtcccgacgt gctcagcggc cggccgtacc gtggcaagcc cagtgacatg tgggccctgg
     7081 gcgtggtgct cttcaccatg ctgtatggcc agttcccctt ctacgacagc atcccgcagg
     7141 agctcttccg caagatcaag gctgccgagt ataccattcc tgaggatgga cgggtttctg
     7201 agaacaccgt gtgtctcatc cggaagctgc tggtccttga cccccagcag cgcctggccg
     7261 ccgccgacgt cctggaggcc ctcagtgcca tcattgcatc atggcagtcc ctgtcatctc
     7321 tgagtgggcc tttgcaggtg gttcctgaca ttgatgacca aatgagcaat gcggatagct
     7381 cccaggaggc gaaggtgacg gaggagtgct cccagtacga gtttgagaac tacatgcgtc
     7441 agcagctgct gctggccgag gagaagagct ccatccatga cgcccggagc tgggtaccca
     7501 agcggcagtt cggcagcgca ccaccggtgc gacggctggg ccacgacgca cagcccatga
     7561 cctccttgga cacggccatc ctggcgcagc gctacctgcg gaaatagcag cttgacccag
     7621 ctttcttgta caaagtggtg atatccagca cagtggcggc cgctcgacaa tcaacctctg
     7681 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     7741 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     7801 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     7861 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     7921 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     7981 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     8041 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     8101 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     8161 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     8221 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattctacc gggtagggga
     8281 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
     8341 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
     8401 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
     8461 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
     8521 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
     8581 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg
     8641 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
     8701 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
     8761 gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc
     8821 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     8881 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     8941 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     9001 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     9061 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     9121 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     9181 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     9241 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     9301 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     9361 tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc
     9421 gaaaggagcg cacgacccca tgcatctcga gggcccggta cctttaagac caatgactta
     9481 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctagc
     9541 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     9601 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     9661 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     9721 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     9781 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     9841 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     9901 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     9961 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
    10021 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
    10081 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
    10141 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
    10201 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
    10261 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
    10321 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
    10381 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
    10441 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
    10501 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
    10561 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
    10621 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
    10681 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
    10741 tttaacaaaa atttaacgcg aattttaaca aaatatta
//
