LOCUS       sequence-335988-       10568 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of TSSK1B (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10568)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-TSSK1B_IDG-K
FEATURES             Location/Qualifiers
     source          1..10568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        126..230
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(231..299,300..1091)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1262..1850
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1751..1770
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2004..2021
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2138..2159
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2174..2179,2180..2197,2198..2204)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2212..2228
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2217..2239
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2236..2252
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2236..2252
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2271..2291
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2273..2291
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2319..2545
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2546..2726
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2773..2898
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3391..3624
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3809..3853
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4120..4236
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4270..4290
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4290..5501
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5338..5357
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(5505..5525)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      5526..5535
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5538..6308
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             6315..6356
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    6357..6381
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7492..7516)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7546..8134
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7599..7619)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8017..8028)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8148..8647
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8183..8206)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8580..8599
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8668..9267
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8668..8687)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9164..9184
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9405..9638
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9710..9831
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9747..9766)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9801..9820
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9871..10006
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10026..10045)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10027..10045)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10055..10072)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10055..10071)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10064..10086)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(10213..10568,1..100)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10300..10319)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10510..10531
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
       61 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
      121 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      181 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
      241 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
      301 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
      361 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
      421 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
      481 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
      541 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
      601 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
      661 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
      721 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
      781 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
      841 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
      901 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
      961 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     1021 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     1081 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     1141 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     1201 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1261 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1321 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1381 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     1441 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1501 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1561 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1621 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     1681 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     1741 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     1801 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     1861 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     1921 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     1981 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     2041 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     2101 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     2161 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     2221 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     2281 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     2341 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     2401 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     2461 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     2521 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     2581 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     2641 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     2701 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     2761 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     2821 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     2881 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     2941 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     3001 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     3061 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     3121 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     3181 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     3241 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     3301 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     3361 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     3421 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     3481 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     3541 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     3601 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     3661 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     3721 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     3781 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     3841 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     3901 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     3961 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     4021 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     4081 cattcgatta gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat
     4141 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     4201 agaattacaa aaacaaatta caaaattcaa aattttatcg tccagtgtgg tggaattctg
     4261 cagatatcac aactttgtat agaaaagttg gcctccgcgc cgggttttgg cgcctcccgc
     4321 gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc
     4381 tgatccttcc gcccggacgc tcaggacagc ggcccgctgc tcataagact cggccttaga
     4441 accccagtat cagcagaagg acattttagg acgggacttg ggtgactcta gggcactggt
     4501 tttctttcca gagagcggaa caggcgagga aaagtagtcc cttctcggcg attctgcgga
     4561 gggatctccg tggggcggtg aacgccgatg attatataag gacgcgccgg gtgtggcaca
     4621 gctagttccg tcgcagccgg gatttgggtc gcggttcttg tttgtggatc gctgtgatcg
     4681 tcacttggtg agtagcgggc tgctgggctg gccggggctt tcgtggccgc cgggccgctc
     4741 ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc gcgagcaagg
     4801 ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg cggctgttcc cgagtcttga
     4861 atggaagacg cttgtgaggc gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg
     4921 gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt attcgggtga
     4981 gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc
     5041 ggtttgtcgt ctgttgcggg ggcggcagtt atggcggtgc cgttgggcag tgcacccgta
     5101 cctttgggag cgcgcgccct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg
     5161 cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg
     5221 gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg
     5281 gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga
     5341 agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg
     5401 caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaat
     5461 tgtccgctaa attctggccg tttttggctt ttttgttaga cggccaactt ttgtatacaa
     5521 agtgggccac catgggaatc ccgctgctga acgctaaaca gattctggga cagctggacg
     5581 gcgggagcgt ggcagtcctg cctgtggtcg actccaccaa tcagtacctg ctggatcgaa
     5641 tcggcgagct gaagagtggg gatgcttgca ttgcagaata tcagcaggca gggagaggaa
     5701 gcagagggag gaaatggttc tctccttttg gagctaacct gtacctgagt atgttttggc
     5761 gcctgaagcg gggaccagca gcaatcggcc tgggcccggt catcggaatt gtcatggcag
     5821 aagcgctgcg aaagctggga gcagacaagg tgcgagtcaa atggcccaat gacctgtatc
     5881 tgcaggatag aaagctggca ggcatcctgg tggagctggc cggaataaca ggcgatgctg
     5941 cacagatcgt cattggcgcc gggattaacg tggctatgag gcgcgtggag gaaagcgtgg
     6001 tcaatcaggg ctggatcaca ctgcaggaag cagggattaa cctggacagg aatactctgg
     6061 ccgctatgct gatccgagag ctgcgggcag ccctggaact gttcgagcag gaaggcctgg
     6121 ctccatatct gtcacggtgg gagaagctgg ataacttcat caatagaccc gtgaagctga
     6181 tcattgggga caaagagatt ttcgggatta gccgggggat tgataaacag ggagccctgc
     6241 tgctggaaca ggacggagtt atcaaaccct ggatgggcgg agaaatcagt ctgcggtctg
     6301 ccgaaaaggc tagcggcaag cccatcccca accccctgct gggcctggac agcaccacaa
     6361 gtttgtacaa aaaagcaggc tccaccatgg atgacgctgc tgtcctcaag cgacgaggct
     6421 acctcctggg gataaattta ggagagggct cctatgcaaa agtaaaatct gcttactctg
     6481 agcgcctgaa gttcaatgtg gcgatcaaga tcatcgaccg caagaaggcc cccgcagact
     6541 tcttggagaa attccttccc cgggaaattg agattctggc catgttaaac cactgctcca
     6601 tcattaagac ctacgagatc tttgagacat cacatggcaa ggtctacatc gtcatggagc
     6661 tcgcggtcca gggcgacctc ctcgagttaa tcaaaacccg gggagccctg catgaggacg
     6721 aagctcgcaa gaagttccac cagctttcct tggccatcaa gtactgccac gacctggacg
     6781 tcgtccaccg ggacctcaag tgtgacaacc ttctccttga caaggacttc aacatcaagc
     6841 tgtccgactt cagcttctcc aagcgctgcc tgcgggatga cagtggtcga atggcattaa
     6901 gcaagacctt ctgtgggtca ccagcgtatg cggccccaga ggtgctgcag ggcattccct
     6961 accagcccaa ggtgtacgac atctggagcc taggcgtgat cctctacatc atggtctgcg
     7021 gctccatgcc ctacgacgac tccaacatca agaagatgct gcgtatccag aaggagcacc
     7081 gcgtcaactt cccacgctcc aagcacctga caggcgagtg caaggacctc atctaccaca
     7141 tgctgcagcc cgacgtcaac cggcggctcc acatcgacga gatcctcagc cactgctgga
     7201 tgcagcccaa ggcacgggga tctccctctg tggccatcaa caaggagggg gagagttccc
     7261 ggggaactga acccttgtgg acccccgaac ctggctctga caagaagtct gccaccaagc
     7321 tggagcctga gggagaggca cagccccagg cacagcctga gacaaaaccc gaggggacag
     7381 caatgcaaat gtccaggcag tcggagatcc tgggtttccc cagcaagccg tcgactatgg
     7441 agacagagga agggcccccc caacagcctc cagagacgcg ggcacagtag gacccagctt
     7501 tcttgtacaa agtggtgata tccagcacag tggcggccgc tcgacaatca acctctggat
     7561 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     7621 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     7681 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     7741 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7801 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7861 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     7921 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     7981 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     8041 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     8101 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttctaccggg taggggaggc
     8161 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     8221 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     8281 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     8341 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     8401 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     8461 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     8521 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     8581 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     8641 cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc
     8701 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     8761 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     8821 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     8881 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     8941 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     9001 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     9061 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     9121 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     9181 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     9241 tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa
     9301 aggagcgcac gaccccatgc atctcgaggg cccggtacct ttaagaccaa tgacttacaa
     9361 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctagctca
     9421 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     9481 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     9541 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     9601 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     9661 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     9721 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     9781 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     9841 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     9901 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     9961 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
    10021 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
    10081 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
    10141 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
    10201 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
    10261 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
    10321 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
    10381 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
    10441 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
    10501 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
    10561 cttttgat
//
