LOCUS       sequence-335990-       10568 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of TSSK3 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10568)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-TSSK3_IDG-K
FEATURES             Location/Qualifiers
     source          1..10568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     294..313
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             382..981
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(382..401)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     878..898
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             1119..1352
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1424..1545
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1461..1480)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1515..1534
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1585..1720
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1740..1759)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1741..1759)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1769..1786)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1769..1785)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1778..1800)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1927..2382
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2014..2033)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2224..2245
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2408..2512
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2513..2581,2582..3373)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3544..4132
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4033..4052
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4286..4303
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4420..4441
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4456..4461,4462..4479,4480..4486)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4494..4510
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4499..4521
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4518..4534
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4518..4534
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4553..4573
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4555..4573
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4601..4827
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4828..5008
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5055..5180
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5673..5906
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6091..6135
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6402..6518
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6552..6572
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6572..7783
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7620..7639
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(7787..7807)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      7808..7817
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7820..8590
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             8597..8638
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    8639..8663
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9774..9798)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9828..10416
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9881..9901)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10299..10310)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        join(10430..10568,1..361)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10465..10488)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
       61 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
      121 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
      181 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct
      241 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg
      301 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc
      361 gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc
      421 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
      481 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
      541 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
      601 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
      661 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
      721 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
      781 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
      841 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
      901 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
      961 acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc
     1021 gcacgacccc atgcatctcg agggcccggt acctttaaga ccaatgactt acaaggcagc
     1081 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctag ctcactccca
     1141 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     1201 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     1261 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     1321 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     1381 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     1441 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     1501 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     1561 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     1621 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     1681 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     1741 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     1801 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     1861 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     1921 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     1981 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     2041 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     2101 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     2161 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     2221 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     2281 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     2341 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     2401 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     2461 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     2521 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     2581 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     2641 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     2701 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     2761 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     2821 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     2881 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     2941 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     3001 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     3061 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     3121 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     3181 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     3241 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     3301 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     3361 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     3421 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     3481 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3541 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3601 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3661 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3721 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3781 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3841 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3901 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3961 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4021 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4081 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4141 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     4201 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     4261 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     4321 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     4381 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     4441 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     4501 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     4561 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
     4621 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
     4681 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
     4741 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
     4801 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
     4861 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     4921 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     4981 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     5041 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     5101 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     5161 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     5221 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     5281 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     5341 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     5401 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     5461 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     5521 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     5581 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     5641 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     5701 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     5761 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     5821 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     5881 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     5941 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     6001 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     6061 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     6121 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     6181 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     6241 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     6301 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     6361 tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag aaaagggggg
     6421 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     6481 aaagaattac aaaaacaaat tacaaaattc aaaattttat cgtccagtgt ggtggaattc
     6541 tgcagatatc acaactttgt atagaaaagt tggcctccgc gccgggtttt ggcgcctccc
     6601 gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt
     6661 cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta
     6721 gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg
     6781 gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg
     6841 gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca
     6901 cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat
     6961 cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc gccgggccgc
     7021 tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa
     7081 ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt cccgagtctt
     7141 gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt
     7201 gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc ttattcgggt
     7261 gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg actggagaac
     7321 tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc agtgcacccg
     7381 tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa
     7441 tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc gcaggacgca
     7501 gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct ggtgagggga
     7561 gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc ttaagtagct
     7621 gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg aagtttttta
     7681 ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt agactagtaa
     7741 attgtccgct aaattctggc cgtttttggc ttttttgtta gacggccaac ttttgtatac
     7801 aaagtgggcc accatgggaa tcccgctgct gaacgctaaa cagattctgg gacagctgga
     7861 cggcgggagc gtggcagtcc tgcctgtggt cgactccacc aatcagtacc tgctggatcg
     7921 aatcggcgag ctgaagagtg gggatgcttg cattgcagaa tatcagcagg cagggagagg
     7981 aagcagaggg aggaaatggt tctctccttt tggagctaac ctgtacctga gtatgttttg
     8041 gcgcctgaag cggggaccag cagcaatcgg cctgggcccg gtcatcggaa ttgtcatggc
     8101 agaagcgctg cgaaagctgg gagcagacaa ggtgcgagtc aaatggccca atgacctgta
     8161 tctgcaggat agaaagctgg caggcatcct ggtggagctg gccggaataa caggcgatgc
     8221 tgcacagatc gtcattggcg ccgggattaa cgtggctatg aggcgcgtgg aggaaagcgt
     8281 ggtcaatcag ggctggatca cactgcagga agcagggatt aacctggaca ggaatactct
     8341 ggccgctatg ctgatccgag agctgcgggc agccctggaa ctgttcgagc aggaaggcct
     8401 ggctccatat ctgtcacggt gggagaagct ggataacttc atcaatagac ccgtgaagct
     8461 gatcattggg gacaaagaga ttttcgggat tagccggggg attgataaac agggagccct
     8521 gctgctggaa caggacggag ttatcaaacc ctggatgggc ggagaaatca gtctgcggtc
     8581 tgccgaaaag gctagcggca agcccatccc caaccccctg ctgggcctgg acagcaccac
     8641 aagtttgtac aaaaaagcag gctccaccat ggatgacgct gctgtcctca agcgacgagg
     8701 ctacctcctg gggataaatt taggagaggg ctcctatgca aaagtaaaat ctgcttactc
     8761 tgagcgcctg aagttcaatg tggcgatcaa gatcatcgac cgcaagaagg cccccgcaga
     8821 cttcttggag aaattccttc cccgggaaat tgagattctg gccatgttaa accactgctc
     8881 catcattaag acctacgaga tctttgagac atcacatggc aaggtctaca tcgtcatgga
     8941 gctcgcggtc cagggcgacc tcctcgagtt aatcaaaacc cggggagccc tgcatgagga
     9001 cgaagctcgc aagaagttcc accagctttc cttggccatc aagtactgcc acgacctgga
     9061 cgtcgtccac cgggacctca agtgtgacaa ccttctcctt gacaaggact tcaacatcaa
     9121 gctgtccgac ttcagcttct ccaagcgctg cctgcgggat gacagtggtc gaatggcatt
     9181 aagcaagacc ttctgtgggt caccagcgta tgcggcccca gaggtgctgc agggcattcc
     9241 ctaccagccc aaggtgtacg acatctggag cctaggcgtg atcctctaca tcatggtctg
     9301 cggctccatg ccctacgacg actccaacat caagaagatg ctgcgtatcc agaaggagca
     9361 ccgcgtcaac ttcccacgct ccaagcacct gacaggcgag tgcaaggacc tcatctacca
     9421 catgctgcag cccgacgtca accggcggct ccacatcgac gagatcctca gccactgctg
     9481 gatgcagccc aaggcacggg gatctccctc tgtggccatc aacaaggagg gggagagttc
     9541 ccggggaact gaacccttgt ggacccccga acctggctct gacaagaagt ctgccaccaa
     9601 gctggagcct gagggagagg cacagcccca ggcacagcct gagacaaaac ccgaggggac
     9661 agcaatgcaa atgtccaggc agtcggagat cctgggtttc cccagcaagc cgtcgactat
     9721 ggagacagag gaagggcccc cccaacagcc tccagagacg cgggcacagt aggacccagc
     9781 tttcttgtac aaagtggtga tatccagcac agtggcggcc gctcgacaat caacctctgg
     9841 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     9901 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     9961 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
    10021 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
    10081 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
    10141 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
    10201 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
    10261 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
    10321 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
    10381 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattctaccg ggtaggggag
    10441 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
    10501 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
    10561 ccgttctt
//
