LOCUS       sequence-335994-       10286 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of TSSK6 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10286)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-TSSK6_IDG-K
FEATURES             Location/Qualifiers
     source          1..10286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     35..54
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             123..722
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(123..142)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     619..639
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             860..1093
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1165..1286
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1202..1221)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1256..1275
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1326..1461
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1481..1500)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1482..1500)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1510..1527)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1510..1526)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1519..1541)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1668..2123
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1755..1774)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1965..1986
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2149..2253
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2254..2322,2323..3114)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3285..3873
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3774..3793
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4027..4044
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4161..4182
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4197..4202,4203..4220,4221..4227)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4235..4251
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4240..4262
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4259..4275
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4259..4275
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4294..4314
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4296..4314
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4342..4568
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4569..4749
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4796..4921
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5414..5647
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5832..5876
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6143..6259
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6293..6313
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6313..7524
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7361..7380
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(7528..7548)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      7549..7558
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7561..8331
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             8338..8379
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    8380..8404
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9233..9257)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9287..9875
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9340..9360)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9758..9769)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        join(9889..10286,1..102)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9924..9947)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg
       61 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta
      121 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
      181 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
      241 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
      301 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
      361 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
      421 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
      481 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
      541 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
      601 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
      661 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
      721 gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatctc
      781 gagggcccgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
      841 ttaaaagaaa aggggggact ggaagggcta gctcactccc aacgaagaca agatctgctt
      901 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
      961 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     1021 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     1081 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     1141 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     1201 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     1261 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     1321 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     1381 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     1441 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     1501 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     1561 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     1621 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     1681 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     1741 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     1801 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     1861 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     1921 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     1981 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     2041 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     2101 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     2161 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     2221 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     2281 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     2341 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     2401 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     2461 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     2521 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     2581 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     2641 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     2701 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     2761 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     2821 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     2881 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     2941 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     3001 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     3061 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     3121 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     3181 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     3241 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3301 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3361 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3421 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3481 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3541 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3601 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3661 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3721 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3781 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3841 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3901 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     3961 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     4021 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     4081 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     4141 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     4201 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     4261 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     4321 aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg
     4381 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
     4441 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
     4501 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
     4561 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     4621 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     4681 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     4741 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     4801 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     4861 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     4921 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     4981 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     5041 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     5101 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     5161 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     5221 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     5281 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     5341 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     5401 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     5461 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     5521 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     5581 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     5641 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     5701 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     5761 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     5821 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     5881 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     5941 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     6001 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     6061 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     6121 gatctcgacg gtatcggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag
     6181 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     6241 ttacaaaatt caaaatttta tcgtccagtg tggtggaatt ctgcagatat cacaactttg
     6301 tatagaaaag ttggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca
     6361 cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg tcctgatcct tccgcccgga
     6421 cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga
     6481 aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg
     6541 gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg
     6601 gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc
     6661 cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg
     6721 ggctgctggg ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg
     6781 tggagagacc gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg
     6841 ttggggggag cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag acgcttgtga
     6901 ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg
     6961 tcttgaggcc ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca
     7021 tctggggacc ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc
     7081 gggggcggca gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc
     7141 cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg
     7201 ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag
     7261 gctctcctga atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag
     7321 tttctttggt cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact
     7381 atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa
     7441 tcatttgggt caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg
     7501 ccgtttttgg cttttttgtt agacggccaa cttttgtata caaagtgggc caccatggga
     7561 atcccgctgc tgaacgctaa acagattctg ggacagctgg acggcgggag cgtggcagtc
     7621 ctgcctgtgg tcgactccac caatcagtac ctgctggatc gaatcggcga gctgaagagt
     7681 ggggatgctt gcattgcaga atatcagcag gcagggagag gaagcagagg gaggaaatgg
     7741 ttctctcctt ttggagctaa cctgtacctg agtatgtttt ggcgcctgaa gcggggacca
     7801 gcagcaatcg gcctgggccc ggtcatcgga attgtcatgg cagaagcgct gcgaaagctg
     7861 ggagcagaca aggtgcgagt caaatggccc aatgacctgt atctgcagga tagaaagctg
     7921 gcaggcatcc tggtggagct ggccggaata acaggcgatg ctgcacagat cgtcattggc
     7981 gccgggatta acgtggctat gaggcgcgtg gaggaaagcg tggtcaatca gggctggatc
     8041 acactgcagg aagcagggat taacctggac aggaatactc tggccgctat gctgatccga
     8101 gagctgcggg cagccctgga actgttcgag caggaaggcc tggctccata tctgtcacgg
     8161 tgggagaagc tggataactt catcaataga cccgtgaagc tgatcattgg ggacaaagag
     8221 attttcggga ttagccgggg gattgataaa cagggagccc tgctgctgga acaggacgga
     8281 gttatcaaac cctggatggg cggagaaatc agtctgcggt ctgccgaaaa ggctagcggc
     8341 aagcccatcc ccaaccccct gctgggcctg gacagcacca caagtttgta caaaaaagca
     8401 ggctccacca tgtcgggaga caaacttctg agcgaactcg gttataagct gggccgcacg
     8461 attggagagg gcagctactc caaggtgaag gtggccacat ccaagaagta caagggtacc
     8521 gtggccatca aggtggtgga ccggcggcga gcgcccccgg acttcgtcaa caagttcctg
     8581 ccgcgagagc tgtccatcct gcggggcgtg cgacacccgc acatcgtgca cgtcttcgag
     8641 ttcatcgagg tgtgcaacgg gaaactgtac atcgtgatgg aagcggccgc caccgacctg
     8701 ctgcaagccg tgcagcgcaa cgggcgcatc cccggagttc aggcgcgcga cctctttgcg
     8761 cagatcgccg gcgccgtgcg ctacctgcac gatcatcacc tggtgcaccg cgacctcaag
     8821 tgcgaaaacg tgctgctgag cccggacgag cgccgcgtca agctcaccga cttcggcttc
     8881 ggccgccagg cccatggcta cccagacctg agcaccacct actgcggctc agccgcctac
     8941 gcgtcacccg aggtgctcct gggcatcccc tacgacccca agaagtacga tgtgtggagc
     9001 atgggcgtcg tgctctacgt catggtcacc gggtgcatgc ccttcgacga ctcggacatc
     9061 gccggcctgc cccggcgcca gaaacgcggc gtgctctatc ccgaaggcct cgagctgtcc
     9121 gagcgctgca aggccctgat cgccgagctg ctgcagttca gcccgtccgc caggccctcc
     9181 gcgggccagg tagcgcgcaa ctgctggctg cgcgccgggg actccggcta ggacccagct
     9241 ttcttgtaca aagtggtgat atccagcaca gtggcggccg ctcgacaatc aacctctgga
     9301 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     9361 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     9421 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     9481 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     9541 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     9601 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     9661 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     9721 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     9781 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     9841 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attctaccgg gtaggggagg
     9901 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
     9961 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
    10021 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
    10081 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
    10141 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
    10201 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
    10261 gcgggctcag gggcgggctc aggggc
//
