LOCUS       sequence-335996-       11009 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of STK33 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11009)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-STK33_IDG-K
FEATURES             Location/Qualifiers
     source          1..11009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     343..363
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             584..817
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    889..1010
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(926..945)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     980..999
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1050..1185
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1205..1224)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1206..1224)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1234..1251)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1234..1250)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1243..1265)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1392..1847
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1479..1498)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1689..1710
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1873..1977
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1978..2046,2047..2838)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3009..3597
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3498..3517
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3751..3768
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3885..3906
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3921..3926,3927..3944,3945..3951)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3959..3975
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3964..3986
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3983..3999
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3983..3999
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4018..4038
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4020..4038
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4066..4292
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4293..4473
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4520..4645
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5138..5371
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5556..5600
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5867..5983
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6017..6037
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6037..7248
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7085..7104
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(7252..7272)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      7273..7282
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7285..8055
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             8062..8103
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    8104..8128
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9680..9704)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9734..10322
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9787..9807)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10205..10216)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        10336..10835
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10371..10394)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10768..10787
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(10856..11009,1..446)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10856..10875)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
       61 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
      121 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
      181 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
      241 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
      301 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
      361 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
      421 gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa
      481 ggagcgcacg accccatgca tctcgagggc ccggtacctt taagaccaat gacttacaag
      541 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctagctcac
      601 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
      661 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      721 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      781 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
      841 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
      901 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
      961 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     1021 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     1081 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     1141 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     1201 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     1261 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     1321 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     1381 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     1441 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     1501 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     1561 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     1621 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     1681 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     1741 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     1801 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     1861 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     1921 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     1981 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2041 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2101 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2161 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2221 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2281 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2341 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     2401 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     2461 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     2521 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     2581 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2641 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     2701 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2761 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     2821 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     2881 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     2941 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3001 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3061 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3121 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     3181 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3241 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3301 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3361 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3421 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3481 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3541 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     3601 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     3661 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     3721 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     3781 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     3841 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     3901 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     3961 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
     4021 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa
     4081 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
     4141 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
     4201 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
     4261 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
     4321 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     4381 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     4441 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     4501 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     4561 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     4621 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     4681 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     4741 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     4801 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     4861 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     4921 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     4981 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     5041 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     5101 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     5161 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     5221 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     5281 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     5341 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     5401 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     5461 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     5521 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     5581 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     5641 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     5701 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     5761 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     5821 acagatccat tcgattagtg aacggatctc gacggtatcg gttaactttt aaaagaaaag
     5881 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     5941 aaactaaaga attacaaaaa caaattacaa aattcaaaat tttatcgtcc agtgtggtgg
     6001 aattctgcag atatcacaac tttgtataga aaagttggcc tccgcgccgg gttttggcgc
     6061 ctcccgcggg cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg
     6121 agcgtcctga tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg
     6181 ccttagaacc ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg
     6241 cactggtttt ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt
     6301 ctgcggaggg atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg
     6361 tggcacagct agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct
     6421 gtgatcgtca cttggtgagt agcgggctgc tgggctggcc ggggctttcg tggccgccgg
     6481 gccgctcggt gggacggaag cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg
     6541 agcaaggttg ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga
     6601 gtcttgaatg gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc
     6661 atggtgggcg gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa agctcttatt
     6721 cgggtgagat gggctggggc accatctggg gaccctgacg tgaagtttgt cactgactgg
     6781 agaactcggt ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc
     6841 acccgtacct ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct
     6901 tataatgcag ggtggggcca cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg
     6961 acgcagggtt cgggcctagg gtaggctctc ctgaatcgac aggcgccgga cctctggtga
     7021 ggggagggat aagtgaggcg tcagtttctt tggtcggttt tatgtaccta tcttcttaag
     7081 tagctgaagc tccggttttg aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt
     7141 ttttaggcac cttttgaaat gtaatcattt gggtcaatat gtaattttca gtgttagact
     7201 agtaaattgt ccgctaaatt ctggccgttt ttggcttttt tgttagacgg ccaacttttg
     7261 tatacaaagt gggccaccat gggaatcccg ctgctgaacg ctaaacagat tctgggacag
     7321 ctggacggcg ggagcgtggc agtcctgcct gtggtcgact ccaccaatca gtacctgctg
     7381 gatcgaatcg gcgagctgaa gagtggggat gcttgcattg cagaatatca gcaggcaggg
     7441 agaggaagca gagggaggaa atggttctct ccttttggag ctaacctgta cctgagtatg
     7501 ttttggcgcc tgaagcgggg accagcagca atcggcctgg gcccggtcat cggaattgtc
     7561 atggcagaag cgctgcgaaa gctgggagca gacaaggtgc gagtcaaatg gcccaatgac
     7621 ctgtatctgc aggatagaaa gctggcaggc atcctggtgg agctggccgg aataacaggc
     7681 gatgctgcac agatcgtcat tggcgccggg attaacgtgg ctatgaggcg cgtggaggaa
     7741 agcgtggtca atcagggctg gatcacactg caggaagcag ggattaacct ggacaggaat
     7801 actctggccg ctatgctgat ccgagagctg cgggcagccc tggaactgtt cgagcaggaa
     7861 ggcctggctc catatctgtc acggtgggag aagctggata acttcatcaa tagacccgtg
     7921 aagctgatca ttggggacaa agagattttc gggattagcc gggggattga taaacaggga
     7981 gccctgctgc tggaacagga cggagttatc aaaccctgga tgggcggaga aatcagtctg
     8041 cggtctgccg aaaaggctag cggcaagccc atccccaacc ccctgctggg cctggacagc
     8101 accacaagtt tgtacaaaaa agcaggctcc accatggctg atagtggctt agataaaaaa
     8161 tccacaaaat gccccgactg ttcatctgct tctcagaaag atgtactttg tgtatgttcc
     8221 agcaaaacaa gggttcctcc agttttggtg gtggaaatgt cacagacatc aagcattggt
     8281 agtgcagaat ctttaatttc actggagaga aaaaaagaaa aaaatatcaa cagagatata
     8341 acctccagga aagatttgcc ctcaagaacc tcaaatgtag agagaaaagc atctcagcaa
     8401 caatggggtc ggggcaactt tacagaagga aaagttcctc acataaggat tgagaatgga
     8461 gctgctattg aggaaatcta tacctttgga agaatattgg gaaaagggag ctttggaata
     8521 gtcattgaag ctacagacaa ggaaacagaa acgaagtggg caattaaaaa agtgaacaaa
     8581 gaaaaggctg gaagctccgc tgtgaagtta cttgaacgag aggtgaacat tctgaaaagt
     8641 gtaaaacatg aacacatcat acatctggaa caagtatttg aaacgccaaa gaaaatgtac
     8701 cttgtgatgg agctttgtga ggatggagaa ctcaaagaaa ttctggatag gaaagggcat
     8761 ttctcagaga atgagacaag gtggatcatt caaagtctcg catcagctat agcatatctt
     8821 cacaataatg atattgtaca tagggatctg aaactggaaa atataatggt taaaagcagt
     8881 cttattgatg ataacaatga aataaactta aacataaagg tgactgattt tggcttagcg
     8941 gtgaagaagc aaagtaggag tgaagccatg ctgcaggcca catgtgggac tcctatctat
     9001 atggcccctg aagttatcag tgcccacgac tatagccagc agtgtgacat ttggagcata
     9061 ggcgtcgtaa tgtacatgtt attacgtgga gaaccaccct ttttggcaag ctcagaagag
     9121 aagctttttg agttaataag aaaaggagaa ctacattttg aaaatgcagt ctggaattcc
     9181 ataagtgact gtgctaaaag tgttttgaaa caacttatga aagtagatcc tgctcacaga
     9241 atcacagcta aggaactact agataaccag tggttaacag gcaataaact ttcttcggtg
     9301 agaccaacca atgtattaga gatgatgaag gaatggaaaa ataacccaga aagtgttgag
     9361 gaaaacacaa cagaagagaa gaataagccg tccactgaag aaaagttgaa aagttaccaa
     9421 ccctggggaa atgtccctga gaccaattac acttcagatg aagaggagga aaaacagtct
     9481 actacttatg aaaagcaatt tcctgcaacc agtaaggaca actttgatat gtgcagttca
     9541 agtttcacat ctagcaaact ccttccagct gaaatcaagg gagaaatgga gaaaacccct
     9601 gtgactccaa gccaaggaac agcaaccaag taccctgcta aatccggcgc cctgtccaga
     9661 accaaaaaga aactctagga cccagctttc ttgtacaaag tggtgatatc cagcacagtg
     9721 gcggccgctc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     9781 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     9841 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     9901 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     9961 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
    10021 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
    10081 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
    10141 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
    10201 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
    10261 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
    10321 gcctggaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
    10381 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
    10441 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
    10501 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
    10561 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
    10621 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
    10681 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
    10741 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
    10801 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatgac
    10861 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
    10921 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
    10981 catcgagcgg gtcaccgagc tgcaagaac
//
