LOCUS       sequence-336004-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This gene was cloned into the gateway middle entry vector. It can be
            used for generating antisense riboprobe or gene expression using a 
            gateway system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Silic MR, Murata SH, Park SJ, Zhang G
  TITLE     Evolution of inwardly rectifying potassium channels and their gene 
            expression in zebrafish embryos.
  JOURNAL   Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.425.
  PUBMED    34558132
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2021 Sep 23. doi: 10.1002/dvdy.425."
COMMENT     Sequence Label: pDONR221-kcnj2a
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    6..105
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             233..244
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 gatggcaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgata
       61 agcaatgctt tcttataatg ccaactttgt acaagaaagc tgggtctcat atttcagatt
      121 cccgccgtag aggccgaggt tccaagggaa cggtggcctg gctgtgttca gagtctgagc
      181 tagtttccgt gtttgttccc ccaggcccca ggctgtcccc gtgcccttct tctttctcct
      241 ctttgtttga aagggccacc tcgttctcat agcaaaatga attagagttg gatatgatgt
      301 actttttctc agcaaggtcc cttgcactgc acagcggggt gctggggact tcgtaggtct
      361 tgtgaaagcg agagtagtct actttgtaat agtttttctc ctcaaagagg actggctcaa
      421 agcggtgtgc ccagaggatt tcattcgcca cataggagct acgacactgg gtggtcattg
      481 ccgtagcctc caccatgccc tccaggatga ccacaatttc gaagtcagag ttttccattt
      541 cttgtttgct catgtcatag aaagggctgt cctcgtcgat ttcatggacg atggtgatgg
      601 gtgacaccaa gaagatgcgg tcaatgccac tgtcgaagcc tacatcaatg tccatctggt
      661 caagagggat aaactctcct tcggctgtgg tgcgagacct gaggagctga gcacggacat
      721 gggcctccac caggtggctt ttgcgcaagt tccccaccct ccacatcana cacagcttat
      781 tgt
//
