LOCUS       sequence-336008-       11096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of TESK2 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11096)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-TESK2_IDG-K
FEATURES             Location/Qualifiers
     source          1..11096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     14..33
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(54..553)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(102..121)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     495..518
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    567..1155
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             673..684
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1082..1102
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    1185..1209
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2848..2872)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(2873..2914)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(2921..3691)
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     regulatory      3694..3703
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    3704..3724
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        complement(3728..4939)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(3872..3891)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(4939..4959)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    4993..5109
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5376..5420)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5605..5838
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6331..6456
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6503..6683
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6684..6910)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(6938..6958)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6938..6956)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6977..6993)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6977..6993)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6990..7012)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7001..7017
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7025..7031,7032..7049,7050..7055))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7070..7091
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7208..7225)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7379..7967)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7459..7478)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8138..8929,8930..8998))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8999..9103)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9129..9584)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9266..9287)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9478..9497
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9711..9733
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9725..9742
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9726..9742
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9752..9771
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9752..9770
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      9791..9926
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9966..10087
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9977..9996)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10031..10050
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             10159..10392
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(join(10530..11096,1..33))
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10613..10633)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 ggcgaggcgc accgtgggct tgtactcggt catggtaagc ttgggctgca ggtcgaaagg
       61 cccggagatg aggaagagga gaacagcgcg gcagacgtgc gcttttgaag cgtgcagaat
      121 gccgggcctc cggaggacct tcgggcgccc gccccgcccc tgagcccgcc cctgagcccg
      181 cccccggacc caccccttcc cagcctctga gcccagaaag cgaaggagca aagctgctat
      241 tggccgctgc cccaaaggcc tacccgcttc cattgctcag cggtgctgtc catctgcacg
      301 agactagtga gacgtgctac ttccatttgt cacgtcctgc acgacgcgag ctgcggggcg
      361 ggggggaact tcctgactag gggaggagta gaaggtggcg cgaaggggcc accaaagaac
      421 ggagccggtt ggcgcctacc ggtggatgtg gaatgtgtgc gaggccagag gccacttgtg
      481 tagcgccaag tgcccagcgg ggctgctaaa gcgcatgctc cagactgcct tgggaaaagc
      541 gcctccccta cccggtagaa ttccaggcgg ggaggcggcc caaagggaga tccgactcgt
      601 ctgagggcga aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg
      661 aaggtccgct ggattgaggg ccgaagggac gtagcagaag gacgtcccgc gcagaatcca
      721 ggtggcaaca caggcgagca gccatggaaa ggacgtcagc ttccccgaca acaccacgga
      781 attgtcagtg cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag
      841 ttccgccgtg gcaataggga gggggaaagc gaaagtcccg gaaaggagct gacaggtggt
      901 ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca gtgcacacca cgccacgttg
      961 cctgacaacg ggccacaact cctcataaag agacagcaac caggatttat acaaggagga
     1021 gaaaatgaaa gccatacggg aagcaatagc atgatacaaa ggcattaaag cagcgtatcc
     1081 acatagcgta aaaggagcaa catagttaag aataccagtc aatctttcac aaattttgta
     1141 atccagaggt tgattgtcga gcggccgcca ctgtgctgga tatcaccact ttgtacaaga
     1201 aagctgggtg gattcaccca tcctgctttc cctgggtttg caggcctatg cctgaggtgg
     1261 agaaggtggc tggagttgag cctgctggcc tttcttctac ctccatctcc tcagaagcac
     1321 ccgcagggca tggactggtc ccctggggcc tggacccaaa accattttct tcctggagaa
     1381 tggagcagtc catagcctca tgggcttgag cagctggtag ggcagatgcc cggaatggtg
     1441 ggatctcttt aactctgtac ttgagactac ttaggcgtgg tgggggccca tcagatagcg
     1501 attcttcccg gcccacaaat ggacaagcct cttgatgcaa gaactcaggc gaaccaggca
     1561 aggaacacca ccggcgaata ggtggggcca ggggctcctg ccagtcagcc aggggcatag
     1621 ttccgggccc tggtgcatcc aggtcaaata ccagagagat gacagacttg ctgggcaggt
     1681 caaaaaactt gatcttgccc cccatgaggt cctggcgagc actaaaaggg ttgactttgg
     1741 gggtgcgggc agcaccatct cgtggccggt agtatgggtc caagacactc actgtacgtg
     1801 ggggcttacg ggaaaagata tctgactggc ttcgagacag ccagatggta cgtcttgggc
     1861 atggtgactt gtgggggatc ttgtcatcca gtgagcttag tcgcttcacc ccaggtgctt
     1921 tctccaagag tcccctggct gtgggctgca gcttcctatc cctctcctgc tcttcttcct
     1981 gtaggcggct cagaatttcc tccagggtct tcccaatctc cacaaaagat gggcgcagtt
     2041 tgggatccat ctctgtgcgg ggaagatagt ccggatcggc ctggatgcgg gcgatgatct
     2101 cgcagaggat gataccataa gagaacacat ctgccttttc attatagggc tcatctcgga
     2161 gaacctcagg tgccatccag aatggggaac ccaccacggc cagcttctca ctccccatgc
     2221 tgacatcggg gatcttctca gccaggccaa agtcagctac cactgcagag taaccattct
     2281 catccctctt tatcaggcag ttcttagatg tgaggtcccg atgaaaaatg cctttgaagt
     2341 gaaggtagct gaggcccact gctatgtcat aggccagttt taccctcaca gtccaaggca
     2401 aatgcaggtt actgtctagc aactgttcca ggttcccgga gttgatatac tctgtaagtg
     2461 catgcaattg tccttgatga acacatacac ccatgaacct aaggatgttg ggatgggaga
     2521 gtctattcat gagctgtact tctttcagca tgtttgcccg gttactgctc aatgtgttca
     2581 tcttaagagc catcacctga ccagaagctc ggtgtcgtac cttgaacact tcagaaaaga
     2641 agccagaccc tattttttca caggtgaaat catccaaacg cgtcagtctg gaaaaggcac
     2701 ttataagagc tcgatacgaa gatggccaaa ctcttcccac ctggctcaca tttccttctc
     2761 ctccaccacc tccttcaaac tcttcaagac gctccacacg tggaggaaat cctgcaattg
     2821 aattccgttt gctccgatcc atggtggagc ctgctttttt gtacaaactt gtggtgctgt
     2881 ccaggcccag cagggggttg gggatgggct tgccgctagc cttttcggca gaccgcagac
     2941 tgatttctcc gcccatccag ggtttgataa ctccgtcctg ttccagcagc agggctccct
     3001 gtttatcaat cccccggcta atcccgaaaa tctctttgtc cccaatgatc agcttcacgg
     3061 gtctattgat gaagttatcc agcttctccc accgtgacag atatggagcc aggccttcct
     3121 gctcgaacag ttccagggct gcccgcagct ctcggatcag catagcggcc agagtattcc
     3181 tgtccaggtt aatccctgct tcctgcagtg tgatccagcc ctgattgacc acgctttcct
     3241 ccacgcgcct catagccacg ttaatcccgg cgccaatgac gatctgtgca gcatcgcctg
     3301 ttattccggc cagctccacc aggatgcctg ccagctttct atcctgcaga tacaggtcat
     3361 tgggccattt gactcgcacc ttgtctgctc ccagctttcg cagcgcttct gccatgacaa
     3421 ttccgatgac cgggcccagg ccgattgctg ctggtccccg cttcaggcgc caaaacatac
     3481 tcaggtacag gttagctcca aaaggagaga accatttcct ccctctgctt cctctccctg
     3541 cctgctgata ttctgcaatg caagcatccc cactcttcag ctcgccgatt cgatccagca
     3601 ggtactgatt ggtggagtcg accacaggca ggactgccac gctcccgccg tccagctgtc
     3661 ccagaatctg tttagcgttc agcagcggga ttcccatggt ggcccacttt gtatacaaaa
     3721 gttggccgtc taacaaaaaa gccaaaaacg gccagaattt agcggacaat ttactagtct
     3781 aacactgaaa attacatatt gacccaaatg attacatttc aaaaggtgcc taaaaaactt
     3841 cacaaaacac actcgccaac cccgagcgca tagttcaaaa ccggagcttc agctacttaa
     3901 gaagataggt acataaaacc gaccaaagaa actgacgcct cacttatccc tcccctcacc
     3961 agaggtccgg cgcctgtcga ttcaggagag cctaccctag gcccgaaccc tgcgtcctgc
     4021 gacggagaaa agcctaccgc acacctaccg gcaggtggcc ccaccctgca ttataagcca
     4081 acagaacggg tgacgtcacg acacgacgag ggcgcgcgct cccaaaggta cgggtgcact
     4141 gcccaacggc accgccataa ctgccgcccc cgcaacagac gacaaaccga gttctccagt
     4201 cagtgacaaa cttcacgtca gggtccccag atggtgcccc agcccatctc acccgaataa
     4261 gagctttccc gcattagcga aggcctcaag accttgggtt cttgccgccc accatgcccc
     4321 ccaccttgtt tcaacgacct cacagcccgc ctcacaagcg tcttccattc aagactcggg
     4381 aacagccgcc attttgctgc gctcccccca acccccagtt cagggcaacc ttgctcgcgg
     4441 acccagacta cagcccttgg cggtctctcc acacgcttcc gtcccaccga gcggcccggc
     4501 ggccacgaaa gccccggcca gcccagcagc ccgctactca ccaagtgacg atcacagcga
     4561 tccacaaaca agaaccgcga cccaaatccc ggctgcgacg gaactagctg tgccacaccc
     4621 ggcgcgtcct tatataatca tcggcgttca ccgccccacg gagatccctc cgcagaatcg
     4681 ccgagaaggg actacttttc ctcgcctgtt ccgctctctg gaaagaaaac cagtgcccta
     4741 gagtcaccca agtcccgtcc taaaatgtcc ttctgctgat actggggttc taaggccgag
     4801 tcttatgagc agcgggccgc tgtcctgagc gtccgggcgg aaggatcagg acgctcgctg
     4861 cgcccttcgt ctgacgtggc agcgctcgcc gtgaggaggg gggcgcccgc gggaggcgcc
     4921 aaaacccggc gcggaggcca acttttctat acaaagttgt gatatctgca gaattccacc
     4981 acactggacg ataaaatttt gaattttgta atttgttttt gtaattcttt agtttgtatg
     5041 tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt
     5101 cttttaaaag ttaaccgata ccgtcgagat ccgttcacta atcgaatgga tctgtctctg
     5161 tctctctctc caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg
     5221 aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt
     5281 acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata
     5341 taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc
     5401 aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt
     5461 aagcttgtgt aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc
     5521 tgttccagag atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt
     5581 tttccagagc aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc
     5641 aggattcttg cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc
     5701 gcctcaatag ccctcagcaa attgttctgc tgctgcacta taccagacaa taattgtctg
     5761 gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac
     5821 ccaaggaaca aagctcctat tcccactgct cttttttctc tctgcaccac tcttctcttt
     5881 gccttggtgg gtgctactcc taatggttca atttttacta ctttatattt atataattca
     5941 cttctccaat tgtccctcat atctcctcct ccaggtctga agatcagcgg ccgcttgctg
     6001 tgcggtggtc ttacttttgt tttgctcttc ctctatcttg tctaaagctt ccttggtgtc
     6061 ttttatctct atcctttgat gcacacaata gagggttgct actgtattat ataatgatct
     6121 aagttcttct gatcctgtct gaagggatgg ttgtagctgt cccagtattt gtctacagcc
     6181 ttctgatgtt tctaacaggc caggattaac tgcgaatcgt tctagctccc tgcttgccca
     6241 tactatatgt tttaatttat attttttctt tccccctggc cttaaccgaa ttttttccca
     6301 tcgcgatcta attctccccc gcttaatact gacgctctcg cacccatctc tctccttcta
     6361 gcctccgcta gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt
     6421 gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt
     6481 caagtccctg ttcgggcgcc actgctagag attttccaca ctgactaaaa gggtctgagg
     6541 gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc
     6601 aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag
     6661 atctggtcta accagagaga cccgtttatg tatcgagcta ggcacttaaa tacaatatct
     6721 ctgcaatgcg gcaattcagt ggttcgtcca atccatgtca gacccgtctg ttgccttcct
     6781 aataaggcac gatcgtacca ccttacttcc accaatcggc atgcacggtg ctttttctct
     6841 ccttgtaagg catgttgcta actcatcgtt accatgttgc aagactacaa gagtattgca
     6901 taagactaca ttaagcttgc agctccagct tttgttccct ttagtgaggg ttaattgcgc
     6961 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     7021 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     7081 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     7141 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     7201 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     7261 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     7321 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     7381 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     7441 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     7501 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     7561 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     7621 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     7681 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     7741 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     7801 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     7861 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     7921 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     7981 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     8041 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     8101 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     8161 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     8221 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     8281 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     8341 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     8401 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     8461 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     8521 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     8581 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     8641 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     8701 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     8761 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     8821 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     8881 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     8941 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9001 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     9061 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     9121 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     9181 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     9241 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     9301 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     9361 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     9421 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     9481 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     9541 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag
     9601 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     9661 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     9721 acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg
     9781 tacgtcccta ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag
     9841 gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg
     9901 gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggatagc tagagccaga
     9961 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
    10021 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
    10081 acaagttcct ctcactctct gatattcatt tctttgcaag ttataaatac tgaataataa
    10141 gatgacatga actactactg ctagagattt tccacactga ctaaaagggt ctgagggatc
    10201 tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc
    10261 tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct
    10321 ggtctaacca gagagaccca gtacaagcaa aaagcagatc ttgtcttcgt tgggagtgag
    10381 ctagcccttc cagtcccccc ttttctttta aaaagtggct aagatctaca gctgccttgt
    10441 aagtcattgg tcttaaaggt accgggccct cgagatgcat ggggtcgtgc gctcctttcg
    10501 gtcgggcgct gcgggtcgtg gggcgggcgt caggcaccgg gcttgcgggt catgcaccag
    10561 gtgcgcggtc cttcgggcac ctcgacgtcg gcggtgacgg tgaagccgag ccgctcgtag
    10621 aaggggaggt tgcggggcgc ggaggtctcc aggaaggcgg gcaccccggc gcgctcggcc
    10681 gcctccactc cggggagcac gacggcgctg cccagaccct tgccctggtg gtcgggcgag
    10741 acgccgacgg tggccaggaa ccacgcgggc tccttgggcc ggtgcggcgc caggaggcct
    10801 tccatctgtt gctgcgcggc cagccgggaa ccgctcaact cggccatgcg cgggccgatc
    10861 tcggcgaaca ccgcccccgc ttcgacgctc tccggcgtgg tccagaccgc caccgcggcg
    10921 ccgtcgtccg cgacccacac cttgccgatg tcgagcccga cgcgcgtgag gaagagttct
    10981 tgcagctcgg tgacccgctc gatgtggcgg tccggatcga cggtgtggcg cgtggcgggg
    11041 tagtcggcga acgcggcggc gagggtgcgt acggccctgg ggacgtcgtc gcgggt
//
