LOCUS       sequence-336009-        6544 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GST-UBXN11 (human) in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6544)
  AUTHORS   Kracht M, van den Boom J, Seiler J, Kroning A, Kaschani F, Kaiser M,
            Meyer H
  TITLE     Protein Phosphatase-1 Complex Disassembly by p97 is Initiated 
            through Multivalent Recognition of Catalytic and Regulatory Subunits
            by the p97 SEP-domain Adapters.
  JOURNAL   J Mol Biol. 2020 Nov 20;432(23):6061-6074. doi: 
            10.1016/j.jmb.2020.10.001. Epub 2020 Oct 12.
  PUBMED    33058883
REFERENCE   2  (bases 1 to 6544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2020.10.001"; journalName: "J Mol Biol"; date: 
            "2020-11-20- 20"; volume: "432"; issue: "23"; pages: "6061-6074"
COMMENT     Sequence Label: pGEX6P1 UBXN11
FEATURES             Location/Qualifiers
     source          1..6544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(81..103)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(248..266)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        334..438
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(439..507,508..1299)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(657..676)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1470..2058
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1959..1978
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2212..2229
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        2302..2379
                     /gene="lacI"
                     /label=lacI promoter
     CDS             2380..3462
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(2399..2418)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(3511..3516,3517..3534,3535..3541)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3549..3565
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3573..3589
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3573..3589
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3585..4112
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGISLSTARCTNASGVR
                     QPSEAVVWLCRS"
     primer_bind     complement(3605..3622)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3605..3621)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3614..3636)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        join(4214..4219,4220..4235,4236..4242)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    4250..4266
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             4289..4942
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     4900..4922
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             4949..4972
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 gaattcccgg gtcgactcga gcggccgcat cgtgactgac tgacgatctg cctcgcgcgt
       61 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
      121 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
      181 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtataa ttcttgaaga
      241 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
      301 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
      361 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      421 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
      481 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
      541 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
      601 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
      661 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
      721 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
      781 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
      841 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
      901 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
      961 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc
     1021 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     1081 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     1141 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     1201 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     1261 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     1321 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     1381 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1441 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1501 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1561 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     1621 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1681 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     1741 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     1801 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     1861 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     1921 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     1981 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     2041 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     2101 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     2161 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     2221 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     2281 atttcacacc gcataaattc cgacaccatc gaatggcgca aaacctttcg cggtatggca
     2341 tgatagcgcc cggaagagag tcaattcagg gtggtgaatg tgaaaccagt aacgttatac
     2401 gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc
     2461 agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac
     2521 attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc
     2581 acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc
     2641 gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt
     2701 aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg
     2761 ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt
     2821 cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg
     2881 cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc
     2941 ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc
     3001 aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa
     3061 caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat
     3121 cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat
     3181 atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc
     3241 accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc
     3301 tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa
     3361 accacgctag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     3421 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     3481 aagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     3541 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgg
     3601 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     3661 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     3721 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgctttgcc tggtttccgg
     3781 caccagaagc ggtgccggaa agctggctgg agtgcgatct tcctgaggcc gatactgtcg
     3841 tcgtcccctc aaactggcag atgcacggtt acgatgcgcc catctacacc aacgtgacct
     3901 atcccattac ggtcaatccg ccgtttgttc ccacggagaa tccgacgggt tgttactcgc
     3961 tcacatttaa tgttgatgaa agctggctac aggaaggcca gacgcgaatt atttttgatg
     4021 gcgttggaat tagcttatcg actgcacggt gcaccaatgc ttctggcgtc aggcagccat
     4081 cggaagctgt ggtatggctg tgcaggtcgt aaatcactgc ataattcgtg tcgctcaagg
     4141 cgcactcccg ttctggataa tgttttttgc gccgacatca taacggttct ggcaaatatt
     4201 ctgaaatgag ctgttgacaa ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgga
     4261 taacaatttc acacaggaaa cagtattcat gtcccctata ctaggttatt ggaaaattaa
     4321 gggccttgtg caacccactc gacttctttt ggaatatctt gaagaaaaat atgaagagca
     4381 tttgtatgag cgcgatgaag gtgataaatg gcgaaacaaa aagtttgaat tgggtttgga
     4441 gtttcccaat cttccttatt atattgatgg tgatgttaaa ttaacacagt ctatggccat
     4501 catacgttat atagctgaca agcacaacat gttgggtggt tgtccaaaag agcgtgcaga
     4561 gatttcaatg cttgaaggag cggttttgga tattagatac ggtgtttcga gaattgcata
     4621 tagtaaagac tttgaaactc tcaaagttga ttttcttagc aagctacctg aaatgctgaa
     4681 aatgttcgaa gatcgtttat gtcataaaac atatttaaat ggtgatcatg taacccatcc
     4741 tgacttcatg ttgtatgacg ctcttgatgt tgttttatac atggacccaa tgtgcctgga
     4801 tgcgttccca aaattagttt gttttaaaaa acgtattgaa gctatcccac aaattgataa
     4861 gtacttgaaa tccagcaagt atatagcatg gcctttgcag ggctggcaag ccacgtttgg
     4921 tggtggcgac catcctccaa aatcggatct ggaagttctg ttccaggggc ccctgggatc
     4981 catgagctca cctttggcct cccttagcaa gacccgaaaa gtgcccctgc cctcggagcc
     5041 tatgaatcct gggaggcgag gaatccgcat ctatggagat gaagatgagg tggacatgtt
     5101 gagtgatggg tgtggctcag aagaaaagat ctcagtccct tcctgctatg gcggcatagg
     5161 tgcccctgtg agtcggcaag tccctgcatc ccatgactcg gagctgatgg ccttcatgac
     5221 gaggaagttg tgggacctgg agcagcaggt gaaggcccag actgatgaga tactgtccaa
     5281 ggatcagaag atagcggccc tagaggacct ggtgcagacc ctccggccac acccagccga
     5341 ggcaaccctg cagcggcagg aggaactgga gacgatgtgt gtgcagctgc agcggcaggt
     5401 cagggagatg gagcggttcc tcagtgacta tggcctgcag tgggtgggcg agcccatgga
     5461 ccaggaggac tcagagagca agacagtctc agagcatggc gagagggact ggatgacagc
     5521 caagaagttc tggaagccag gggactcatt ggcgccccct gaggtggact ttgacaggct
     5581 gctggccagc ctgcaggatc ttagtgagct ggtggtagag ggtgacaccc aagtgacacc
     5641 agtgcccggc ggggcacggc tgcgtaccct cgagcccatc ccgctgaagc tctaccggaa
     5701 tggcatcatg atgttcgacg ggcccttcca gcccttctac gatccctcca cacagcgctg
     5761 cctccgagac atattggatg gcttctttcc ctcagagctc cagcgactgt accccaatgg
     5821 ggtccccttt aaggtgagtg acttgcgcaa tcaggtctac ctggaggatg gactggaccc
     5881 cttcccaggc gagggccgtg tggtgggcag gcagctgatg cacaaggcct tggacagggt
     5941 ggaggagcac ccaggctcca ggatgactgc tgagaaattt ctgaacaggc tccccaagtt
     6001 tgtgatccgg caaggcgagg tgattgacat ccggggcccc atcagggaca ccttgcagaa
     6061 ctgctgccca ttgcctgccc ggatccagga gattgtggtg gagacgccca ccttggccgc
     6121 tgagcgagag aggagccagg agtcacccaa cacgccggca cccccgctct ccatgctgcg
     6181 catcaagtct gagaatgggg aacaggcctt cctactgatg atgcagcctg acaacaccat
     6241 tggggacgtg cgagctctgc tagcgcaggc cagggtcatg gatgcctctg cctttgagat
     6301 cttcagcaca ttcccgccca ccctctacca ggacgataca ctcacgctgc aggctgcagg
     6361 ccttgtgccc aaagcagcac tgctgctgcg ggcacgccga gccccgaagt ccagcctgaa
     6421 attcagtcct ggtccctgtc ccggtcccgg tcccggcccc agtcccggtc ccggtcccgg
     6481 ccccagtccc ggtcccggtc ccggccccag tccctgtcct ggacccagtc ccagccccca
     6541 atag
//
