LOCUS       sequence-33602-e         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, 
            Eichtinger M, Jiang T, Foley JE, Winfrey RJ, Townsend JA, 
            Unger-Wallace E, Sander JD, Muller-Lerch F, Fu F, Pearlberg J, 
            Goebel C, Dassie JP, Pruett-Miller SM, Porteus MH, Sgroi DC, Iafrate
            AJ, Dobbs D, McCray PB, Cathomen T, Voytas DF, Joung JK
  TITLE     Rapid ""open-source"" engineering of customized zinc-finger 
            nucleases for highly efficient gene modification.
  JOURNAL   Mol Cell. 2008 Jul 25. 31(2):294-301.
  PUBMED    18657511
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2008 Jul 25. 31(2):294-301."
COMMENT     Sequence Label: zebrafish similar to gpr151 (or gpcr-2037)_R (...
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..71
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(560..579)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tacattgtgc aaaggccctg agaaaaatcc aagctttggc ggccgctgac tacaaagacg
       61 atgatgacaa atctagaccc ggggagcgcc ccttccagtg tcgcatttgc atgcggaact
      121 tttcggacct gagcacgctt cggcggcata cccgtactca taccggtgaa aaaccgtttc
      181 agtgtcggat ctgtatgcga aatttctccc agaacgagca cttgaaggtc catctacgta
      241 cgcacaccgg cgagaagcca ttccaatgcc gaatatgcat gcgcaacttc agtcagaaca
      301 gccacctgcg ccggcaccta aaaacccacc tgaggggatc ctctgcacag tagtaactag
      361 agactagaaa aaggccgaca agtcccgctc cgctgaagat cctggcgtaa tagcgaagag
      421 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
      481 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
      541 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
      601 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
      661 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
      721 tgatagacgg tttttcgccc t
//
