LOCUS       sequence-336021-        8517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of un-tagged human androgen receptor 
            phosphorylation mutant: AR-Ser81Ala (AR-S81A) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8517)
  TITLE     Balk lab plasmids
REFERENCE   2  (bases 1 to 8517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCIneo-AR-S81A
FEATURES             Location/Qualifiers
     source          1..8517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    4221..4342
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4232..4251)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4286..4305
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      4528..4983
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4615..4634)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4825..4846
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5100..5457
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5100..5291
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5123..5143)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5308..5443
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5508..6302
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5562..5581)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6172..6191
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6366..6414
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(6455..6474)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6574..6596
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6634..6652)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6720..6824
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6825..6893,6894..7685)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7043..7062)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7856..8444
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8345..8364
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc gtttaaactt aagcttggta ccgagctcgg atccatggaa gtgcagttag
     1141 ggctgggaag ggtctaccct cggccgccgt ccaagaccta ccgaggagct ttccagaatc
     1201 tgttccagag cgtgcgcgaa gtgatccaga acccgggccc caggcaccca gaggccgcga
     1261 gcgcagcacc tcccggcgcc agtttgctgc tgctgcagca gcagcagcag cagcagcagc
     1321 agcagcagca gcagcagcag caagagactg cccccaggca gcagcagcag cagcagggtg
     1381 aggatggttc tccccaagcc catcgtagag gccccacagg ctacctggtc ctggatgagg
     1441 aacagcaacc ttcacagccg cagtcggccc tggagtgcca ccccgagaga ggttgcgtcc
     1501 cagagcctgg agccgccgtg gccgccagca aggggctgcc gcagcagctg ccagcacctc
     1561 cggacgagga tgactcagct gccccatcca cgttgtccct gctgggcccc actttccccg
     1621 gcttaagcag ctgctccgct gaccttaaag acatcctgag cgaggccagc accatgcaac
     1681 tccttcagca acagcagcag gaagcagtat ccgaaggcag cagcagcggg agagcgaggg
     1741 aggcctcggg ggctcccact tcctccaagg acaattactt agggggcact tcgaccattt
     1801 ctgacaacgc caaggagttg tgtaaggcag tgtcggtgtc catgggcctg ggtgtggagg
     1861 cgttggagca tctgagtcca ggggaacagc ttcgggggga ttgcatgtac gccccacttt
     1921 tgggagttcc acccgctgtg cgtcccactc cttgtgcccc attggccgaa tgcaaaggtt
     1981 ctctgctaga cgacagcgca ggcaagagca ctgaagatac tgctgagtat tcccctttca
     2041 agggaggtta caccaaaggg ctagaaggcg agagcctagg ctgctctggc agcgctgcag
     2101 cagggagctc cgggacactt gaactgccgt ctaccctgtc tctctacaag tccggagcac
     2161 tggacgaggc agctgcgtac cagagtcgcg actactacaa ctttccactg gctctggccg
     2221 gaccgccgcc ccctccgccg cctccccatc cccacgctcg catcaagctg gagaacccgc
     2281 tggactacgg cagcgcctgg gcggctgcgg cggcgcagtg ccgctatggg gacctggcga
     2341 gcctgcatgg cgcgggtgca gcgggacccg gttctgggtc accctcagcc gccgcttcct
     2401 catcctggca cactctcttc acagccgaag aaggccagtt gtatggaccg tgtggtggtg
     2461 gtgggggtgg tggtggcggc ggcggcggcg gcggcggcgg cggcggcggc ggcggcggcg
     2521 gcggcggcgg cggcgaggcg ggagctgtag ccccctacgg ctacactcgg ccccctcagg
     2581 ggctggcggg ccaggaaagc gacttcaccg cacctgatgt gtggtaccct ggcggcatgg
     2641 tgagcagagt gccctatccc agtcccactt gtgtcaaaag cgaaatgggc ccctggatgg
     2701 atagctactc cggaccttac ggggacatgc gtttggagac tgccagggac catgttttgc
     2761 ccattgacta ttactttcca ccccagaaga cctgcctgat ctgtggagat gaagcttctg
     2821 ggtgtcacta tggagctctc acatgtggaa gctgcaaggt cttcttcaaa agagccgctg
     2881 aagggaaaca gaagtacctg tgcgccagca gaaatgattg cactattgat aaattccgaa
     2941 ggaaaaattg tccatcttgt cgtcttcgga aatgttatga agcagggatg actctgggag
     3001 cccggaagct gaagaaactt ggtaatctga aactacagga ggaaggagag gcttccagca
     3061 ccaccagccc cactgaggag acaacccaga agctgacagt gtcacacatt gaaggctatg
     3121 aatgtcagcc catctttctg aatgtcctgg aagccattga gccaggtgta gtgtgtgctg
     3181 gacacgacaa caaccagccc gactcctttg cagccttgct ctctagcctc aatgaactgg
     3241 gagagagaca gcttgtacac gtggtcaagt gggccaaggc cttgcctggc ttccgcaact
     3301 tacacgtgga cgaccagatg gctgtcattc agtactcctg gatggggctc atggtgtttg
     3361 ccatgggctg gcgatccttc accaatgtca actccaggat gctctacttc gcccctgatc
     3421 tggttttcaa tgagtaccgc atgcacaagt cccggatgta cagccagtgt gtccgaatga
     3481 ggcacctctc tcaagagttt ggatggctcc aaatcacccc ccaggaattc ctgtgcatga
     3541 aagcactgct actcttcagc attattccag tggatgggct gaaaaatcaa aaattctttg
     3601 atgaacttcg aatgaactac atcaaggaac tcgatcgtat cattgcatgc aaaagaaaaa
     3661 atcccacatc ctgctcaaga cgcttctacc agctcaccaa gctcctggac tccgtgcagc
     3721 ctattgcgag agagctgcat cagttcactt ttgacctgct aatcaagtca cacatggtga
     3781 gcgtggactt tccggaaatg atggcagaga tcatctctgt gcaagtgccc aagatccttt
     3841 ctgggaaagt caagcccatc tatttccaca cccagtgaag cattggaaac cctatttccc
     3901 caccccagct catgccccct ttcagatgtc ttctgcctgt tataactctg cactactcct
     3961 ctgcagtgcc ttggggaatt tcctctattg atgtacagtc tgtcatgaac atgttcctga
     4021 attctatttg ctgggctttt tttttctctt tctctccttt ctttttcttc ttccctccct
     4081 atctaaccct cccatggcac cttcagactt tgcttcccat tgtggctcct atctgtgttt
     4141 tgaatggtgt tgtatgcctt tatccagcac agtggcggcc gcttcccttt agtgagggtt
     4201 aatgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     4261 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     4321 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     4381 gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatccg
     4441 ataaggatcg atccgggctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     4501 cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg
     4561 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4621 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4681 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     4741 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     4801 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     4861 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     4921 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     4981 tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgcgg
     5041 atctgcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt aggtaccttc
     5101 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     5161 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     5221 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5281 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5341 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     5401 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     5461 cttgattctt ctgacacaac agtctcgaac ttaaggctag agccaccatg attgaacaag
     5521 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     5581 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     5641 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     5701 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     5761 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     5821 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     5881 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     5941 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     6001 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     6061 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     6121 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     6181 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     6241 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     6301 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgatg
     6361 gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga
     6421 tagcgataag gatccgcgta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     6481 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     6541 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     6601 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     6661 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     6721 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6781 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     6841 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     6901 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     6961 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     7021 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     7081 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     7141 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     7201 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     7261 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     7321 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     7381 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     7441 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     7501 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     7561 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7621 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7681 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     7741 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     7801 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7861 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7921 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7981 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     8041 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     8101 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     8161 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8221 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     8281 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8341 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     8401 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8461 cctttttacg gttcctggcc ttttgctggc cttttgctca catggctcga cagatct
//
