LOCUS       sequence-336037-        8520 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of un-tagged human androgen receptor 
            phosphorylation mutant: AR-Ser650Ala (AR-S650A) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8520)
  TITLE     Balk lab plasmids
REFERENCE   2  (bases 1 to 8520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCIneo-AR-S650A
FEATURES             Location/Qualifiers
     source          1..8520
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    4224..4345
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4235..4254)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4289..4308
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      4531..4986
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4618..4637)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4828..4849
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5103..5460
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5103..5294
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5126..5146)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5311..5446
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5511..6305
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5565..5584)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6175..6194
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6369..6417
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(6458..6477)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6577..6599
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6637..6655)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6723..6827
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6828..6896,6897..7688)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7046..7065)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7859..8447
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8348..8367
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc gtttaaactt aagcttggta ccgagctcgg atccatggaa gtgcagttag
     1141 ggctgggaag ggtctaccct cggccgccgt ccaagaccta ccgaggagct ttccagaatc
     1201 tgttccagag cgtgcgcgaa gtgatccaga acccgggccc caggcaccca gaggccgcga
     1261 gcgcagcacc tcccggcgcc agtttgctgc tgctgcagca gcagcagcag cagcagcagc
     1321 agcagcagca gcagcagcag cagcaagaga ctagccccag gcagcagcag cagcagcagg
     1381 gtgaggatgg ttctccccaa gcccatcgta gaggccccac aggctacctg gtcctggatg
     1441 aggaacagca accttcacag ccgcagtcgg ccctggagtg ccaccccgag agaggttgcg
     1501 tcccagagcc tggagccgcc gtggccgcca gcaaggggct gccgcagcag ctgccagcac
     1561 ctccggacga ggatgactca gctgccccat ccacgttgtc cctgctgggc cccactttcc
     1621 ccggcttaag cagctgctcc gctgacctta aagacatcct gagcgaggcc agcaccatgc
     1681 aactccttca gcaacagcag caggaagcag tatccgaagg cagcagcagc gggagagcga
     1741 gggaggcctc gggggctccc acttcctcca aggacaatta cttagggggc acttcgacca
     1801 tttctgacaa cgccaaggag ttgtgtaagg cagtgtcggt gtccatgggc ctgggtgtgg
     1861 aggcgttgga gcatctgagt ccaggggaac agcttcgggg ggattgcatg tacgccccac
     1921 ttttgggagt tccacccgct gtgcgtccca ctccttgtgc cccattggcc gaatgcaaag
     1981 gttctctgct agacgacagc gcaggcaaga gcactgaaga tactgctgag tattcccctt
     2041 tcaagggagg ttacaccaaa gggctagaag gcgagagcct aggctgctct ggcagcgctg
     2101 cagcagggag ctccgggaca cttgaactgc cgtctaccct gtctctctac aagtccggag
     2161 cactggacga ggcagctgcg taccagagtc gcgactacta caactttcca ctggctctgg
     2221 ccggaccgcc gccccctccg ccgcctcccc atccccacgc tcgcatcaag ctggagaacc
     2281 cgctggacta cggcagcgcc tgggcggctg cggcggcgca gtgccgctat ggggacctgg
     2341 cgagcctgca tggcgcgggt gcagcgggac ccggttctgg gtcaccctca gccgccgctt
     2401 cctcatcctg gcacactctc ttcacagccg aagaaggcca gttgtatgga ccgtgtggtg
     2461 gtggtggggg tggtggtggc ggcggcggcg gcggcggcgg cggcggcggc ggcggcggcg
     2521 gcggcggcgg cggcggcgag gcgggagctg tagcccccta cggctacact cggccccctc
     2581 aggggctggc gggccaggaa agcgacttca ccgcacctga tgtgtggtac cctggcggca
     2641 tggtgagcag agtgccctat cccagtccca cttgtgtcaa aagcgaaatg ggcccctgga
     2701 tggatagcta ctccggacct tacggggaca tgcgtttgga gactgccagg gaccatgttt
     2761 tgcccattga ctattacttt ccaccccaga agacctgcct gatctgtgga gatgaagctt
     2821 ctgggtgtca ctatggagct ctcacatgtg gaagctgcaa ggtcttcttc aaaagagccg
     2881 ctgaagggaa acagaagtac ctgtgcgcca gcagaaatga ttgcactatt gataaattcc
     2941 gaaggaaaaa ttgtccatct tgtcgtcttc ggaaatgtta tgaagcaggg atgactctgg
     3001 gagcccggaa gctgaagaaa cttggtaatc tgaaactaca ggaggaagga gaggcttcca
     3061 gcaccaccgc ccccactgag gagacaaccc agaagctgac agtgtcacac attgaaggct
     3121 atgaatgtca gcccatcttt ctgaatgtcc tggaagccat tgagccaggt gtagtgtgtg
     3181 ctggacacga caacaaccag cccgactcct ttgcagcctt gctctctagc ctcaatgaac
     3241 tgggagagag acagcttgta cacgtggtca agtgggccaa ggccttgcct ggcttccgca
     3301 acttacacgt ggacgaccag atggctgtca ttcagtactc ctggatgggg ctcatggtgt
     3361 ttgccatggg ctggcgatcc ttcaccaatg tcaactccag gatgctctac ttcgcccctg
     3421 atctggtttt caatgagtac cgcatgcaca agtcccggat gtacagccag tgtgtccgaa
     3481 tgaggcacct ctctcaagag tttggatggc tccaaatcac cccccaggaa ttcctgtgca
     3541 tgaaagcact gctactcttc agcattattc cagtggatgg gctgaaaaat caaaaattct
     3601 ttgatgaact tcgaatgaac tacatcaagg aactcgatcg tatcattgca tgcaaaagaa
     3661 aaaatcccac atcctgctca agacgcttct accagctcac caagctcctg gactccgtgc
     3721 agcctattgc gagagagctg catcagttca cttttgacct gctaatcaag tcacacatgg
     3781 tgagcgtgga ctttccggaa atgatggcag agatcatctc tgtgcaagtg cccaagatcc
     3841 tttctgggaa agtcaagccc atctatttcc acacccagtg aagcattgga aaccctattt
     3901 ccccacccca gctcatgccc cctttcagat gtcttctgcc tgttataact ctgcactact
     3961 cctctgcagt gccttgggga atttcctcta ttgatgtaca gtctgtcatg aacatgttcc
     4021 tgaattctat ttgctgggct ttttttttct ctttctctcc tttctttttc ttcttccctc
     4081 cctatctaac cctcccatgg caccttcaga ctttgcttcc cattgtggct cctatctgtg
     4141 ttttgaatgg tgttgtatgc ctttatccag cacagtggcg gccgcttccc tttagtgagg
     4201 gttaatgctt cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag
     4261 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     4321 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     4381 tcagggggag atgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat
     4441 ccgataagga tcgatccggg ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     4501 caacagttgc gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg
     4561 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     4621 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4681 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4741 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4801 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4861 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     4921 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     4981 caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
     5041 cggatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg gttaggtacc
     5101 ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     5161 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     5221 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     5281 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     5341 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     5401 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     5461 aagcttgatt cttctgacac aacagtctcg aacttaaggc tagagccacc atgattgaac
     5521 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     5581 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     5641 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     5701 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     5761 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     5821 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     5881 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     5941 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     6001 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     6061 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     6121 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     6181 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     6241 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     6301 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     6361 atggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat
     6421 cgatagcgat aaggatccgc gtatggtgca ctctcagtac aatctgctct gatgccgcat
     6481 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     6541 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     6601 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     6661 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     6721 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     6781 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     6841 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     6901 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     6961 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     7021 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     7081 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     7141 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     7201 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     7261 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     7321 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     7381 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     7441 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     7501 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     7561 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     7621 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     7681 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     7741 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     7801 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     7861 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7921 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7981 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     8041 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8101 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8161 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8221 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     8281 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8341 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8401 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8461 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatggct cgacagatct
//
