LOCUS       sequence-33605-e         601 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, 
            Eichtinger M, Jiang T, Foley JE, Winfrey RJ, Townsend JA, 
            Unger-Wallace E, Sander JD, Muller-Lerch F, Fu F, Pearlberg J, 
            Goebel C, Dassie JP, Pruett-Miller SM, Porteus MH, Sgroi DC, Iafrate
            AJ, Dobbs D, McCray PB, Cathomen T, Voytas DF, Joung JK
  TITLE     Rapid ""open-source"" engineering of customized zinc-finger 
            nucleases for highly efficient gene modification.
  JOURNAL   Mol Cell. 2008 Jul 25. 31(2):294-301.
  PUBMED    18657511
REFERENCE   2  (bases 1 to 601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2008 Jul 25. 31(2):294-301."
COMMENT     Sequence Label: xylt1_L (OZ509)
FEATURES             Location/Qualifiers
     source          1..601
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..57
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(546..565)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggccctgaga aaatccaagc tttggcggcc gctgactaca aagacgatga tgacaaatct
       61 agacccgggg agcgcccctt ccagtgtcgc atttgcatgc ggaacttttc gaccacgacc
      121 aagcttaacg tgcatacccg tactcatacc ggtgaaaaac cgtttcagtg tcggatctgt
      181 atgcgaaatt tctccagggc cgacaacttg ctgaggcatc tacgtacgca caccggcgag
      241 aagccattcc aatgccgaat atgcatgcgc aacttcagtc agagcacgag cctgcagcgc
      301 cacctaaaaa cccacctgag gggatcctct gcacagtagt aactagagac tagaaaaagg
      361 ccgacaagtc ccgctccgct gaagatcctg gcgtaatagc gaagaggccc gcaccgatcg
      421 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
      481 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
      541 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
      601 a
//
