LOCUS       sequence-336057-        7857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of nerfin-3UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7857)
  AUTHORS   Eulalio A, Rehwinkel J, Stricker M, Huntzinger E, Yang SF, Doerks T,
            Dorner S, Bork P, Boutros M, Izaurralde E
  TITLE     Target-specific requirements for enhancers of decapping in 
            miRNA-mediated gene silencing.
  JOURNAL   Genes Dev. 2007 Oct 15. 21(20):2558-70.
  PUBMED    17901217
REFERENCE   2  (bases 1 to 7857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2007 Oct 15. 21(20):2558-70."
COMMENT     Sequence Label: pAc5.1C-Fluc-nerfin_E
FEATURES             Location/Qualifiers
     source          1..7857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2453..4108
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MAEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVA
                     PANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQ
                     SMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSH
                     ARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDY
                     KIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQG
                     YGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIM
                     SGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESI
                     LLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGV
                     VFVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(2522..2540)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     rep_origin      complement(5907..6495)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5987..6006)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6666..7457,7458..7526))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7289..7308
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7527..7631)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7699..7717
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(7781..7857,1..2433)
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
ORIGIN
        1 gatctcgctg cctgttatgt ggcccacaaa ccaagacacg ttttatggcc attaaagctg
       61 gctgatcgtc gccaaacacc aaatacataa tgaatatgta cacattcgag aaagaagcga
      121 tcaaagaagc gtcttcgggc ggagtaggag aatgcggagg agaaggagaa cgagctgatc
      181 tagtatctct ccacaatcca atgccaactg accaactggc catattcgga gcaatttgaa
      241 gccaatttcc atcgcctggc gatcgctcca ttcttggcta tatgtttttc accgttaccc
      301 ggggccattt tcaaagactc gtcggcaaga taagattgtg tcactcgctg tctctcttca
      361 tttgtcgaag aatgctgagg aatttcgcga tgacgtcggc gagtattttg aagaatgaga
      421 ataatttgta tttatacgaa aatcagttag tggaattttc tacaaaaaca tgttatctat
      481 agataatttt gttgcaaaat atgttgacta tgacaaagat tgtatgtata tacctttaat
      541 gtattctcat tttcttatgt atttataatg gcaatgatga tactgatgat attttaagat
      601 gatgccagac caaaaggctt gaatttctgc gtcttttgcc gaacgcagtg catgtgcaat
      661 tgttgttttt tggaatattc aattttcgga ctgtccgctt tgatttcagt ttcttggctt
      721 attcaaaaag caaagtaaag ccaaaaaagc gagatggcaa taccaaatgc ggcaaaacgg
      781 tagtggaagg aaaggggtgc ggggcagcgg aaggaagggt ggggcggggc gtggcggggt
      841 ctgtggctgg gcgcgacgtc accgacgttg gagccactcc tttgaccatg tgtgcgtgtg
      901 tgtattattc gtgtctcgcc actcgccggt tgtttttttc tttttatgct gcgctctctc
      961 tagcgccatc tcgcttacgc atgctcaacg caccgcatgt tgccgtttcc ttttatgcgt
     1021 cattttggct cgaaataggc aattatttaa acaaagatta gtcaacgaaa acgctaaaat
     1081 aaataagtct acaatatggt tacttattgc catgtgtgtg cagccaacga tagcaacaaa
     1141 agcaacaaca caggtggctt tccctctttc actttttgtt tgcaagccgc gtgcgagcaa
     1201 gacggcacga ccggcaaacg caattacgct gacaaagagc agacgaagtt ttggcgaaaa
     1261 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1321 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1381 aattaggaat cggacgctta tcgttagggt aacaacaaga aatgcttact gagtcacagc
     1441 ctctggaaaa ctgccgcaag ccagagagag agagaaaaag agggagagca gcttagaccg
     1501 catgtgcttg tgtgtgaggc gtctctctct tcgtctctgt tgcgcaaacg catagactgc
     1561 actgaaaaaa tcgattacct attttttatg aatgaatatt tgcactatta ctattcaaaa
     1621 ctattaagat agcaatcaca ttcaatagcc aaatactata ccacctgagc gatgcaacga
     1681 aatgatcaat ttgagcaaaa atgctgcata tttaggacgg catcattata gaaatgcttc
     1741 ttgctgtgta cttttctctc gtctggcagc tgtttcgccg ttattgttaa aaccggctta
     1801 agttaggtgt gttttctacg actagtgaat gccctactag aagatgtgtg ttgcacaaaa
     1861 tgtccctgga ataaccaatt tgaagtgcag atagcagtaa acgtaagcta atatgaatat
     1921 tatttaactg taatgtttta atatcgctgg acattactaa taaacccact ataaacacat
     1981 gtacatatgt atgttttggc atacaatgag tagttgggga aaaaatgtgt aaaagcaccg
     2041 tgaccatcac agcataaaga taaccagctg aagtatcgaa tatgagtaac ccccaaattg
     2101 aatcacatgc cgcaactgat aggacccatg gaagtacact cttcatggcg atatacaaga
     2161 cacacacaag cacgaacacc cagttgcgga ggaaattctc cgtaaatgaa aacccaatcg
     2221 gcgaacaatt catacccata tatggtaaaa gttttgaacg cgacttgaga gcggagagca
     2281 ttgcggctga taaggtttta gcgctaagcg ggctttataa aacgggctgc gggaccagtt
     2341 ttcatatcac taccgtttga gttcttgtgc tgtgtggata ctcctcccga cacaaagccg
     2401 ctccatcagc cagcagtcgt ctaatccaga gaccccggat cggggtacca acatggccga
     2461 agacgccaaa aacataaaga aaggcccggc accattctat ccactagagg atggaaccgc
     2521 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt
     2581 tacagatgca catatcgagg tgaacatcac gtacgcggaa tacttcgaaa tgtccgttcg
     2641 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag
     2701 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt
     2761 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatga acatttcgca
     2821 gcctaccgta gtgtttgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa
     2881 attaccaata atccagaaaa ttattatcat ggattctaaa acggattacc agggatttca
     2941 gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt
     3001 accagagtcc tttgatcgtg acaaaacaat tgcactgata atgaactcct ctggatctac
     3061 tgggttacct aagggtgtgg cccttccgca tagaactgcc tgcgtcagat tctcgcatgc
     3121 cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc
     3181 attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt
     3241 cgtcttaatg tatagatttg aagaagagct gtttttacga tcccttcagg attacaaaat
     3301 tcaaagtgcg ttgctagtac caaccctatt ttcattcttc gccaaaagca ctctgattga
     3361 caaatacgat ttatctaatt tacacgaaat tgcttctggg ggcgcacctc tttcgaaaga
     3421 agtcggggaa gcggttgcaa aacgcttcca tcttccaggg atacgacaag gatatgggct
     3481 cactgagact acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt
     3541 cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct
     3601 gggcgttaat cagagaggcg aattatgtgt cagaggacct atgattatgt ccggttatgt
     3661 aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga
     3721 catagcttac tgggacgaag acgaacactt cttcatagtt gaccgcttga agtctttaat
     3781 taaatacaaa gggtatcagg tggcccccgc tgaattggaa tcgatattgt tacaacaccc
     3841 caacatcttc gacgcgggcg tggcaggtct tcccgacgat gacgccggtg aacttcccgc
     3901 cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt
     3961 cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt
     4021 accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc
     4081 caagaagggc ggaaagtcca aattgtaaga attctttagc tagtttagga acttttggga
     4141 gtgcaatgaa cttacaacga aagccaaagc aaccaaccaa agatatataa ctaatactag
     4201 tcagtcatat atccttctac tcctttgctc cgacatcatc tcatgacatc attcatcacc
     4261 catatcatcc ttcattcttc ctactactaa tcatcccata gcttttcaca ctttcatact
     4321 aatttcctca tgactcactc tcattctgta tcatctttgc attttttgca aatcattttc
     4381 cagttaatat tctacccaaa attcgaccct ttaacaactt tttcaatctg gaaatttctc
     4441 gtcattctag tcacaagttc ttgcattcct tctagtcact tagggtaaat catctctata
     4501 agtcagcttc agttcgatct tactaagttt gtacaaacat atatcgttct tcaatttcgg
     4561 atacagcttt agtcatattc aggttatata ccgaaaccaa aattcaggca aaattgtgca
     4621 gtaatagtgt ttaagttcga atcaaaccaa aaagcaaacc aaatcctagt ttaaagcaaa
     4681 ttcaagccca gtgtttgtga accaaagtcg agtgtgagct ctagtcattt tagccaccta
     4741 atcaagcgga atcagcttgt tctcagtcta gtttagttag tttagttagg catagaaacg
     4801 gacattggac cagcgtctca ctaccagtca aagtattgaa cagtacaacc tagctataag
     4861 ggccacaggc ttcagaaccg gcggttgtgt acttaaagca ataaaataag taatccccga
     4921 tatcgggaca atatcacagc gctattgttc cttagcatca gtatgtgtaa attggttgta
     4981 accgcagagt acaacgaaaa gggaaacttt cgctcaatct aggaattaac taactctaag
     5041 ctatatcata catgtaacca tatcggttga gattttgtga tactacagtc taagcttcaa
     5101 gtttacaaga gaatcgttgc gctcgattgt gcattaacaa taagctaaga agagacagat
     5161 cattgaacca agtattttta tacgtaatat acaccatata tttttataca aaaaaaaact
     5221 cttgaagacc ttttttaatt atatgatgtc taagatgtaa tcgacatata tttatcggaa
     5281 accaatatat ttttatacaa aacccaatct tggaaaaccc ataaaaattg taaattataa
     5341 aagctcttga ataaaaacgc aaaactgaaa atgaaaactt cttgttgggt tcttttgggt
     5401 tgtcagggaa aacgtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg
     5461 tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc
     5521 gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc
     5581 gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc
     5641 ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg
     5701 gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc
     5761 cccattatga ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg
     5821 tccaggcagg tagatgacga ccatcaggga cagcttcaag gccagcaaaa ggccaggaac
     5881 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5941 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6001 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6061 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6121 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6181 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6241 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6301 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     6361 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6421 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6481 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6541 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6601 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6661 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     6721 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6781 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     6841 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6901 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6961 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7021 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7081 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     7141 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7201 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7261 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
     7321 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7381 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7441 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7501 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7561 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7621 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     7681 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattaatt
     7741 ctatattcta aaaacacaaa tgatacttct aaaaaaaaat catgaatggc atcaactctg
     7801 aatcaaatct ttgcagatgc acctacttct catttccact gtcacatcat ttttcca
//
