LOCUS       sequence-336058-        9890 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For bacterial expression of AsCas12a D908A (RuvC-inactivating 
            mutation) with an N-terminal His-MBP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9890)
  AUTHORS   Cofsky JC, Karandur D, Huang CJ, Witte IP, Kuriyan J, Doudna JA
  TITLE     CRISPR-Cas12a exploits R-loop asymmetry to form double-strand 
            breaks.
  JOURNAL   Elife. 2020 Jun 10;9. pii: 55143. doi: 10.7554/eLife.55143.
  PUBMED    32519675
REFERENCE   2  (bases 1 to 9890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-06-10"; volume: "9. pii: 55143. doi"; pages: " 
            10.7554/eLife.55143"
COMMENT     Sequence Label: pJCC_075 AsCas12a D908A
FEATURES             Location/Qualifiers
     source          1..9890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(40..58)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        126..230
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(231..299,300..1091)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(449..468)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1262..1850
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1751..1770
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2004..2021
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2036..2175
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2101..2120)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2261..2283
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2277..2468)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     3926..3945
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4029..4048
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4029..4047
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             4136..4158
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4178..4207
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             4217..5317
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     5291..5314
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             5372..5392
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(9448..9466)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9462..9509
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(9875..9880,9881..9890,1..7,8..13))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt
       61 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
      121 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      181 tgagacaata accctgataa atgcttcaat aacattgaaa aaggaagagt atgagtattc
      241 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
      301 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
      361 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
      421 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
      481 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
      541 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
      601 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
      661 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
      721 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa
      781 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
      841 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
      901 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
      961 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     1021 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     1081 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     1141 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     1201 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1261 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1321 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1381 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     1441 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1501 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1561 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1621 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     1681 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     1741 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     1801 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     1861 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     1921 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     1981 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     2041 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt gcactctcag
     2101 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
     2161 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     2221 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     2281 aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg
     2341 tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc
     2401 agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt
     2461 ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg
     2521 aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa
     2581 cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag
     2641 ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat
     2701 cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt
     2761 tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag
     2821 cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa
     2881 ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag
     2941 gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat
     3001 atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca
     3061 attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg
     3121 cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta
     3181 caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca
     3241 gcggtccaat gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct
     3301 gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc
     3361 cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca
     3421 agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac
     3481 gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg
     3541 caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc
     3601 agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg
     3661 cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg
     3721 gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt
     3781 tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca
     3841 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
     3901 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
     3961 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc
     4021 cgcgaaatta atacgactca ctatagggag accacaacgg tttccctcta gtgccggctc
     4081 cggagagctc tttaattaag cggccgccct gcaggactcg agttctagaa ataattttgt
     4141 ttaactttaa gaaggagata tacatatgaa atcttctcac catcaccatc accatcacca
     4201 tcaccatggt tcttctatga aaatcgaaga aggtaaactg gtaatctgga ttaacggcga
     4261 taaaggctat aacggtctcg ctgaagtcgg taagaaattc gagaaagata ccggaattaa
     4321 agtcaccgtt gagcatccgg ataaactgga agagaaattc ccacaggttg cggcaactgg
     4381 cgatggccct gacattatct tctgggcaca cgaccgcttt ggtggctacg ctcaatctgg
     4441 cctgttggct gaaatcaccc cggacaaagc gttccaggac aagctgtatc cgtttacctg
     4501 ggatgccgta cgttacaacg gcaagctgat tgcttacccg atcgctgttg aagcgttatc
     4561 gctgatttat aacaaagatc tgctgccgaa cccgccaaaa acctgggaag agatcccggc
     4621 gctggataaa gaactgaaag cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc
     4681 gtacttcacc tggccgctga ttgctgctga cgggggttat gcgttcaagt atgaaaacgg
     4741 caagtacgac attaaagacg tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt
     4801 cctggttgac ctgattaaaa acaaacacat gaatgcagac accgattact ccatcgcaga
     4861 agctgccttt aataaaggcg aaacagcgat gaccatcaac ggcccgtggg catggtccaa
     4921 catcgacacc agcaaagtga attatggtgt aacggtactg ccgaccttca agggtcaacc
     4981 atccaaaccg ttcgttggcg tgctgagcgc aggtattaac gccgccagtc cgaacaaaga
     5041 gctggcaaaa gagttcctcg aaaactatct gctgactgat gaaggtctgg aagcggttaa
     5101 taaagacaaa ccgctgggtg ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga
     5161 tccacgtatt gccgccacta tggaaaacgc ccagaaaggt gaaatcatgc cgaacatccc
     5221 gcagatgtcc gctttctggt atgccgtgcg tactgcggtg atcaacgccg ccagcggtcg
     5281 tcagactgtc gatgaagccc tgaaagacgc gcagactaat tcgagctcga acaacaacaa
     5341 caataacaat aacaacaacc tcgggatcga ggaaaacctg tacttccaat ccaatgcaat
     5401 gacccagttc gaggggttta ccaatctgta tcaagtgagc aagacgctgc gctttgaact
     5461 gatcccacag ggaaaaacct taaaacatat tcaagagcag ggctttatcg aagaagataa
     5521 ggcccgtaat gaccattaca aagagttaaa gccgattatt gatcgtatct acaagaccta
     5581 tgcggaccag tgcttacaat tggtacagct tgattgggag aacctctctg ccgccatcga
     5641 ttcctatcgt aaagaaaaaa ctgaagaaac gcgcaacgcc ctgattgaag agcaggccac
     5701 ctatcgtaac gcgattcatg actattttat tggccgtacg gacaatctga cggacgcgat
     5761 caacaagcgc catgcggaga tttacaaagg actgtttaag gctgaactgt tcaatggtaa
     5821 ggtccttaaa cagcttggga ccgtcacaac gacggaacat gaaaacgcgt tattacgtag
     5881 cttcgacaag tttaccacgt atttctccgg cttttacgaa aatcgcaaaa acgttttcag
     5941 tgccgaggat atttccactg ctatccctca tcgcattgtg caagacaact tcccaaaatt
     6001 caaagaaaat tgtcatatct tcacccgctt aatcaccgct gtaccgtccc tgcgtgagca
     6061 tttcgaaaac gtgaaaaagg ccattggtat cttcgtgtct acttcgattg aggaggtatt
     6121 ttcctttcca ttctataatc agctgctgac ccagacccaa attgatctgt acaaccagct
     6181 gcttggcggt atttctcgtg aagcaggaac cgaaaaaatc aaagggttga acgaggtgct
     6241 taatctggca atccagaaaa atgatgaaac cgcccacatc attgcttcgt tacctcatcg
     6301 ttttatcccg ttgttcaagc aaattttaag tgatcgcaat acgctgtcgt ttattctgga
     6361 agaattcaaa agtgatgaag aggtaattca gtcgttttgc aaatataaaa ccctgttacg
     6421 taacgaaaat gtcctggaaa cagccgaggc tttgtttaac gaactgaata gcattgacct
     6481 gacgcatatc tttattagcc acaaaaaatt agagaccatc tcatcagctc tgtgcgatca
     6541 ttgggataca ctgcgcaatg cgctgtatga acgtcgtatt tcggaattga ctggcaaaat
     6601 cactaaaagc gcgaaagaga aagtacagcg ctcgcttaaa catgaagata tcaacctgca
     6661 ggagatcatc agcgccgcgg gtaaagaact gtcggaggca tttaaacaga agacgagcga
     6721 gattctgtcc cacgcacatg ccgccttaga ccagccgctc ccgaccactc tgaagaaaca
     6781 ggaagagaaa gaaatcctta aaagtcaact ggacagttta ctgggtctct atcatctgct
     6841 ggattggttt gcggtagacg aaagcaatga agtggatccg gagtttagtg cccgtctgac
     6901 aggaatcaag ctggaaatgg agccttcgct tagcttctac aacaaagccc gcaattatgc
     6961 cacgaaaaaa ccctatagtg tcgaaaaatt taaactcaac tttcaaatgc cgacccttgc
     7021 gtcgggctgg gatgtcaaca aagaaaaaaa caacggagct attctgttcg ttaaaaatgg
     7081 tctgtactac ctgggcatca tgccgaaaca gaaaggtcgc tacaaagccc tttcgttcga
     7141 gcccacggaa aaaacaagcg aaggcttcga caaaatgtac tacgattact ttccggatgc
     7201 agcaaaaatg atcccgaaat gttccacaca gctgaaagcc gttacagcac attttcagac
     7261 gcacaccacc cccatcttac tgtccaacaa ttttattgaa ccgctggaga ttactaaaga
     7321 aatttatgat ttgaacaatc cggaaaaaga gccaaaaaag tttcaaaccg cctacgctaa
     7381 aaaaaccggg gatcagaaag ggtaccgcga agcgttgtgc aagtggattg atttcacccg
     7441 cgattttctc agtaaatata ccaagactac ctcgattgac ctgagctcac tgcgcccgag
     7501 ctctcaatat aaggatttgg gtgagtacta tgctgaatta aaccctttat tgtaccacat
     7561 ttcttttcag cgcatcgccg aaaaggaaat tatggacgca gtcgaaaccg ggaaactgta
     7621 cctgttccag atctataata aggacttcgc caaaggacat catggcaaac cgaacctgca
     7681 caccctttac tggaccgggc ttttctctcc ggaaaatttg gcgaaaacct cgatcaagct
     7741 taacggtcaa gctgagctgt tttaccgtcc aaaatcccgc atgaagcgca tggcgcatcg
     7801 tttaggtgaa aaaatgctga ataagaaact gaaagatcag aaaaccccta tcccggatac
     7861 cctctaccag gaactgtatg attacgtgaa ccatcgtctc tcgcatgacc tgtcagacga
     7921 agcgcgtgcg ttactgccca atgtaatcac aaaagaagtt tcgcatgaaa ttattaaaga
     7981 tcgtcgtttt acatctgata aattcttttt tcatgttccg atcaccctca actatcaggc
     8041 cgcaaacagt ccaagtaagt ttaaccagcg cgttaatgct tacctgaagg aacatccgga
     8101 gactccgatt attggaattg ctcgcggtga acgtaatttg atctatatca ctgtgatcga
     8161 tagtaccggt aagattctgg agcagcgcag cttgaacaca attcaacagt ttgattatca
     8221 gaaaaaatta gacaaccgcg aaaaagagcg cgtggctgcc cgtcaggcgt ggtctgttgt
     8281 cggtaccatt aaagatctga agcagggcta tctttctcag gttattcacg aaattgtaga
     8341 tctgatgatc cattatcagg cggttgttgt gttggagaat ctcaatttcg gttttaagag
     8401 taagcgcaca ggcatcgctg aaaaagcagt ttatcagcag tttgaaaaaa tgctgatcga
     8461 caaattgaac tgtttagttc tcaaagatta cccagcggaa aaggtgggcg gagtgctgaa
     8521 tccgtaccaa ttaacggatc aattcacttc cttcgcaaag atgggtaccc aaagcggctt
     8581 tctgttctat gtgccggccc cgtatacctc gaaaatcgat ccactgacgg gcttcgtaga
     8641 tccgttcgtg tggaaaacca ttaaaaatca tgaaagtcgt aaacattttc tcgaaggctt
     8701 cgacttcctg cactacgacg tgaaaactgg cgatttcatt ctgcatttta aaatgaaccg
     8761 caacctttcg tttcagcgcg gtctgccggg ctttatgccg gcttgggaca ttgtttttga
     8821 gaaaaatgaa acccagtttg atgctaaagg cactcctttc atcgccggta aacgcatcgt
     8881 acctgtgatt gaaaaccatc gttttacagg gcgttaccgt gatttatacc cggcgaacga
     8941 attgatcgcg ctgctggagg aaaagggcat cgttttccgt gacggctcca atattctgcc
     9001 gaaattactg gaaaacgacg attcacacgc aattgatacc atggtcgcac tgattcgctc
     9061 agtcttacag atgcgtaact ctaatgcagc cacaggagaa gattatatta attcgccagt
     9121 ccgcgatttg aacggtgttt gcttcgacag ccgttttcag aatcctgaat ggccgatgga
     9181 cgctgatgcc aacggagctt atcatatcgc cctgaaaggc cagctcctgc tgaaccacct
     9241 gaaggaaagc aaagatctga aattgcagaa cggcattagc aaccaggact ggttagcata
     9301 catccaggaa ctgcgtaact aataacattg gaagtggata acggatccgc gatcgcggcg
     9361 cgccacctgg tggccggccg gtaccacgcg tgcgcgctga tccggctgct aacaaagccc
     9421 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
     9481 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac
     9541 aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc
     9601 aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat
     9661 tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg
     9721 acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc
     9781 agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga
     9841 ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat
//
