LOCUS       sequence-33606-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, 
            Eichtinger M, Jiang T, Foley JE, Winfrey RJ, Townsend JA, 
            Unger-Wallace E, Sander JD, Muller-Lerch F, Fu F, Pearlberg J, 
            Goebel C, Dassie JP, Pruett-Miller SM, Porteus MH, Sgroi DC, Iafrate
            AJ, Dobbs D, McCray PB, Cathomen T, Voytas DF, Joung JK
  TITLE     Rapid ""open-source"" engineering of customized zinc-finger 
            nucleases for highly efficient gene modification.
  JOURNAL   Mol Cell. 2008 Jul 25. 31(2):294-301.
  PUBMED    18657511
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2008 Jul 25. 31(2):294-301."
COMMENT     Sequence Label: cftr_L (OZ517)
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..75
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     rep_origin      477..835
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(564..583)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     774..795
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggaaatcatt gtgcaaaggc cctgagaaaa atccaagctt tggcggccgc tgactacaaa
       61 gacgatgatg acaaatctag acccggggag cgccccttcc agtgtcgcat ttgcatgcgg
      121 aacttttcgg cgaacagcca gcttacccgc catacccgta ctcataccgg tgaaaaaccg
      181 tttcagtgtc ggatctgtat gcgaaatttc tccgtccaga gcagcttgag ccgccatcta
      241 cgtacgcaca ccggcgagaa gccattccaa tgccgaatat gcatgcgcaa cttcagtcgg
      301 ctcgacatgc tggcccgcca cctaaaaacc cacctgaggg gatcctctgc acagtagtaa
      361 ctagagacta gaaaaaggcc gacaagtccc gctccgctga agatcctggc gtaatagcga
      421 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
      481 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
      541 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
      601 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
      661 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
      721 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
      781 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
      841 attgcatcgc
//
