LOCUS       sequence-336066-         719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector to express sgRNA resistant 3XFLAG tagged human 
            CHP1 with myristoylation site mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Zhu XG, Nicholson Puthenveedu S, Shen Y, La K, Ozlu C, Wang T, 
            Klompstra D, Gultekin Y, Chi J, Fidelin J, Peng T, Molina H, Hang 
            HC, Min W, Birsoy K
  TITLE     CHP1 Regulates Compartmentalized Glycerolipid Synthesis by 
            Activating GPAT4.
  JOURNAL   Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi: 
            10.1016/j.molcel.2019.01.037. Epub 2019 Mar 4.
  PUBMED    30846317
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2019.01.037"; journalName: "Mol Cell"; date: 
            "2019-04-4- 4"; volume: "74"; issue: "1"; pages: "45-58.e7"
COMMENT     Sequence Label: pMXS-IRES-Blast 3XFLAG-CHP1-myr_mut
FEATURES             Location/Qualifiers
     source          1..719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             633..698
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 gccggatcta gctagttaat taagccacca tggcttctcg ggccgcaacg ttactgcggg
       61 acgaagagct cgaggagatc aagaaggaga ccggctttag tcattcgcaa atcactcgcc
      121 tctacagccg gttcaccagc ctggacaaag gagagaatgg gactctcagc cgggaagatt
      181 tccagaggat tccagaactt gccatcaacc cactggggga ccggatcatc aatgccttct
      241 ttccagaggg agaggaccag gtaaacttcc gtggattcat gcgaactttg gctcatttcc
      301 gccccattga ggataatgaa aagagcaaag atgtgaatgg acccgaacca ctcaacagcc
      361 gaagcaacaa actgcacttt gcttttcgac tatatgattt ggataaagat gaaaagatct
      421 cccgtgatga gctgttacaa gttttgagga tgatggtagg agtaaatatc tcagatgagc
      481 agctgggcag catcgcagac aggaccattc aggaggctga tcaggatggg gacagtgcca
      541 tatctttcac agaatttgtt aaggttttgg agaaggtgga tgtagaacag aaaatgagca
      601 tccgatttct tcacggaggt tcaggtggat cagactacaa agaccatgac ggtgattata
      661 aagatcatga cattgattac aaggatgacg atgacaagtg agctacgtaa attccgccc
//
