LOCUS       sequence-336085-       10865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of RUNX1 V92E mutant protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, 
            Hutter S, Haferlach T, Ideker T, Mali P, Carter H
  TITLE     Interface-guided phenotyping of coding variants in the transcription
            factor RUNX1.
  JOURNAL   Cell Rep. 2024 Jul 4;43(7):114436. doi: 
            10.1016/j.celrep.2024.114436.
  PUBMED    38968069
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-4"; volume: "43(7):114436. doi"; pages: " 
            10.1016/j.celrep.2024.114436"
COMMENT     Sequence Label: EF1A_Hygro_P2A_RUNX1_V92E_Barcode
FEATURES             Location/Qualifiers
     source          1..10865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(8..28)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        13..342
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      193..328
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        390..437
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             456..830
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    960..1081
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(997..1016)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1051..1070
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1130..1146)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1130..1146)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1143..1165)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1154..1170
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1178..1184,1185..1202,1203..1208))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1223..1244
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1361..1378)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1532..2120)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1612..1631)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2291..3082,3083..3151))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2914..2933
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3152..3256)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3331..3350)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3522..3901
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3903..4101
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4056..4076
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4119..4299
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4346..4471
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4964..5197
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5382..5426
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5724..5841
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5977..7155
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6208..7146
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7103..7123
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     7172..7191
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7172..7190
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7254..8276
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7254..8276
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8286..8342
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(9896..9929)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             9954..10134
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10160..10177)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10166..10390
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10436..10864
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10523..10542)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10733..10754
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
       61 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
      121 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
      181 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
      241 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
      301 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
      361 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg
      421 gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc
      481 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
      541 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
      601 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
      661 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
      721 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
      781 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac
      841 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
      901 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
      961 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     1021 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     1081 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     1141 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     1201 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     1261 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     1321 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     1381 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1441 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1501 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1561 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1621 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1681 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1741 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1801 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1861 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1921 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     1981 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2041 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2101 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2161 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2221 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2281 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2341 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2401 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     2461 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2521 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     2581 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     2641 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     2701 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     2761 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     2821 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     2881 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     2941 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3001 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     3061 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3121 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3181 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3241 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
     3301 atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     3361 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
     3421 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
     3481 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
     3541 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     3601 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     3661 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     3721 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     3781 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     3841 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     3901 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     3961 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     4021 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     4081 gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga
     4141 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     4201 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     4261 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     4321 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     4381 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     4441 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     4501 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     4561 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     4621 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     4681 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     4741 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     4801 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     4861 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     4921 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     4981 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     5041 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     5101 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     5161 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     5221 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     5281 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     5341 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     5401 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     5461 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     5521 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     5581 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     5641 acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga
     5701 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     5761 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     5821 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta
     5881 attaatcgat aagctttgca aagatggata aagttttaaa cagagaggaa tctttgcagc
     5941 taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca
     6001 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt
     6061 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     6121 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     6181 cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc
     6241 ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg
     6301 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
     6361 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
     6421 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
     6481 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
     6541 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
     6601 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
     6661 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc
     6721 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
     6781 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
     6841 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
     6901 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg
     6961 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
     7021 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
     7081 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
     7141 tttcaggtgt cgtgaggaat tagcttggta ctaatacgac tcactatagg gagacccaag
     7201 ctggctaggt aagcttgata tcgaattcct gcagcccggg ggatcccgcc accatgaaaa
     7261 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
     7321 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
     7381 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
     7441 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     7501 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     7561 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
     7621 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     7681 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     7741 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     7801 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     7861 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     7921 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     7981 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     8041 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     8101 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     8161 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     8221 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaaggat
     8281 ccggcgcaac aaacttctct ctgctgaaac aagccggaga tgtcgaagag aatcctggac
     8341 cgctgcgtat ccccgtagat gccagcacga gccgccgctt cacgccgcct tccaccgcgc
     8401 tgagcccagg caagatgagc gaggcgttgc cgctgggcgc cccggacgcc ggcgctgccc
     8461 tggccggcaa gctgaggagc ggcgaccgca gcatggtgga ggtgctggcc gaccacccgg
     8521 gcgagctggt gcgcaccgac agccccaact tcctctgctc cgtgctgcct acgcactggc
     8581 gctgcaacaa gaccctgccc atcgctttca aggtggaggc cctaggggat gttccagatg
     8641 gcactctggt cactgtgatg gctggcaatg atgaaaacta ctcggctgag ctgagaaatg
     8701 ctaccgcagc catgaagaac caggttgcaa gatttaatga cctcaggttt gtcggtcgaa
     8761 gtggaagagg gaaaagcttc actctgacca tcactgtctt cacaaaccca ccgcaagtcg
     8821 ccacctacca cagagccatc aaaatcacag tggatgggcc ccgagaacct cgaagacatc
     8881 ggcagaaact agatgatcag accaagcccg ggagcttgtc cttttccgag cggctcagtg
     8941 aactggagca gctgcggcgc acagccatga gggtcagccc acaccaccca gcccccacgc
     9001 ccaaccctcg tgcctccctg aaccactcca ctgcctttaa ccctcagcct cagagtcaga
     9061 tgcaggatac aaggcagatc caaccatccc caccgtggtc ctacgatcag tcctaccaat
     9121 acctgggatc cattgcctct ccttctgtgc acccagcaac gcccatttca cctggacgtg
     9181 ccagcggcat gacaaccctc tctgcagaac tttccagtcg actctcaacg gcacccgacc
     9241 tgacagcgtt cagcgacccg cgccagttcc ccgcgctgcc ctccatctcc gacccccgca
     9301 tgcactatcc aggcgccttc acctactccc cgacgccggt cacctcgggc atcggcatcg
     9361 gcatgtcggc catgggctcg gccacgcgct accacaccta cctgccgccg ccctaccccg
     9421 gctcgtcgca agcgcaggga ggcccgttcc aagccagctc gccctcctac cacctgtact
     9481 acggcgcctc ggccggctcc taccagttct ccatggtggg cggcgagcgc tcgccgccgc
     9541 gcatcctgcc gccctgcacc aacgcctcca ccggctccgc gctgctcaac cccagcctcc
     9601 cgaaccagag cgacgtggtg gaggccgagg gcagccacag caactccccc accaacatgg
     9661 cgccctccgc gcgcctggag gaggccgtgt ggaggcccta ctgactagct aagttagaac
     9721 tatttcctgg ctgttacgcg ttggaatata ttctactgac gggcttgtta aacagtgaac
     9781 cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatcgaac
     9841 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atatcataac
     9901 ttcgtatagc atacattata cgaagttatc gtctagctgg tccggactgt actgggtctc
     9961 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
    10021 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
    10081 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
    10141 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
    10201 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
    10261 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
    10321 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
    10381 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
    10441 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
    10501 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
    10561 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
    10621 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
    10681 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
    10741 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
    10801 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
    10861 aatta
//
