LOCUS       sequence-336088-       10865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of RUNX1 N109Y mutant protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, 
            Hutter S, Haferlach T, Ideker T, Mali P, Carter H
  TITLE     Interface-guided phenotyping of coding variants in the transcription
            factor RUNX1.
  JOURNAL   Cell Rep. 2024 Jul 4;43(7):114436. doi: 
            10.1016/j.celrep.2024.114436.
  PUBMED    38968069
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-4"; volume: "43(7):114436. doi"; pages: " 
            10.1016/j.celrep.2024.114436"
COMMENT     Sequence Label: EF1A_Hygro_P2A_RUNX1_N109Y_Barcode
FEATURES             Location/Qualifiers
     source          1..10865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(294..327)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             352..532
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(558..575)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    564..788
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      834..1262
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(921..940)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1131..1152
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1271..1291)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1276..1605
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1456..1591
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        1653..1700
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1719..2093
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    2223..2344
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2260..2279)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2314..2333
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2393..2409)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2393..2409)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2406..2428)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2417..2433
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2441..2447,2448..2465,2466..2471))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2486..2507
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2624..2641)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2795..3383)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2875..2894)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3554..4345,4346..4414))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4177..4196
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4415..4519)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4594..4613)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4785..5164
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5166..5364
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5319..5339
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             5382..5562
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5609..5734
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6227..6460
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6645..6689
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6987..7104
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7240..8418
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7471..8409
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8366..8386
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     8435..8454
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8435..8453
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             8517..9539
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8517..9539
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             9549..9605
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 aaccagagcg acgtggtgga ggccgagggc agccacagca actcccccac caacatggcg
       61 ccctccgcgc gcctggagga ggccgtgtgg aggccctact gactagctaa gttagaacta
      121 tttcctggct gttacgcgta atgttcatac ctactgacgg gcttgttaaa cagtgaaccg
      181 cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcgaacaa
      241 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atcataactt
      301 cgtatagcat acattatacg aagttatcgt ctagctggtc cggactgtac tgggtctctc
      361 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      421 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      481 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt
      541 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
      601 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
      661 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
      721 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
      781 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
      841 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
      901 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
      961 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     1021 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     1081 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     1141 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     1201 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     1261 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     1321 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     1381 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     1441 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     1501 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     1561 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     1621 tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata
     1681 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg
     1741 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
     1801 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
     1861 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
     1921 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
     1981 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
     2041 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc
     2101 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     2161 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     2221 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     2281 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     2341 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     2401 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     2461 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     2521 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     2581 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     2641 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     2701 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2761 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2821 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2881 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2941 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3001 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3061 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3121 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3181 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     3241 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3301 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3361 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3421 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3481 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3541 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     3601 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     3661 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     3721 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     3781 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     3841 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     3901 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     3961 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     4021 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4081 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4141 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     4201 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4261 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     4321 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4381 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     4441 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     4501 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     4561 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     4621 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
     4681 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
     4741 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
     4801 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     4861 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     4921 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     4981 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     5041 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     5101 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     5161 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     5221 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     5281 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     5341 acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc
     5401 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     5461 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     5521 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     5581 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     5641 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     5701 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     5761 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     5821 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     5881 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     5941 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     6001 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     6061 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     6121 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     6181 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     6241 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     6301 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     6361 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     6421 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     6481 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     6541 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     6601 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     6661 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     6721 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     6781 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     6841 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     6901 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc
     6961 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     7021 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     7081 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
     7141 ttaattaatc gataagcttt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     7201 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     7261 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     7321 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     7381 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     7441 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     7501 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     7561 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     7621 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     7681 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     7741 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     7801 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     7861 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     7921 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     7981 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     8041 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     8101 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     8161 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     8221 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     8281 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     8341 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     8401 ccatttcagg tgtcgtgagg aattagcttg gtactaatac gactcactat agggagaccc
     8461 aagctggcta ggtaagcttg atatcgaatt cctgcagccc gggggatccc gccaccatga
     8521 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
     8581 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
     8641 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
     8701 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
     8761 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
     8821 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
     8881 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
     8941 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
     9001 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
     9061 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
     9121 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
     9181 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
     9241 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc
     9301 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
     9361 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
     9421 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
     9481 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaag
     9541 gatccggcgc aacaaacttc tctctgctga aacaagccgg agatgtcgaa gagaatcctg
     9601 gaccgatgcg tatccccgta gatgccagca cgagccgccg cttcacgccg ccttccaccg
     9661 cgctgagccc aggcaagatg agcgaggcgt tgccgctggg cgccccggac gccggcgctg
     9721 ccctggccgg caagctgagg agcggcgacc gcagcatggt ggaggtgctg gccgaccacc
     9781 cgggcgagct ggtgcgcacc gacagcccca acttcctctg ctccgtgctg cctacgcact
     9841 ggcgctgcaa caagaccctg cccatcgctt tcaaggtggt ggccctaggg gatgttccag
     9901 atggcactct ggtcactgtg atggctggct atgatgaaaa ctactcggct gagctgagaa
     9961 atgctaccgc agccatgaag aaccaggttg caagatttaa tgacctcagg tttgtcggtc
    10021 gaagtggaag agggaaaagc ttcactctga ccatcactgt cttcacaaac ccaccgcaag
    10081 tcgccaccta ccacagagcc atcaaaatca cagtggatgg gccccgagaa cctcgaagac
    10141 atcggcagaa actagatgat cagaccaagc ccgggagctt gtccttttcc gagcggctca
    10201 gtgaactgga gcagctgcgg cgcacagcca tgagggtcag cccacaccac ccagccccca
    10261 cgcccaaccc tcgtgcctcc ctgaaccact ccactgcctt taaccctcag cctcagagtc
    10321 agatgcagga tacaaggcag atccaaccat ccccaccgtg gtcctacgat cagtcctacc
    10381 aatacctggg atccattgcc tctccttctg tgcacccagc aacgcccatt tcacctggac
    10441 gtgccagcgg catgacaacc ctctctgcag aactttccag tcgactctca acggcacccg
    10501 acctgacagc gttcagcgac ccgcgccagt tccccgcgct gccctccatc tccgaccccc
    10561 gcatgcacta tccaggcgcc ttcacctact ccccgacgcc ggtcacctcg ggcatcggca
    10621 tcggcatgtc ggccatgggc tcggccacgc gctaccacac ctacctgccg ccgccctacc
    10681 ccggctcgtc gcaagcgcag ggaggcccgt tccaagccag ctcgccctcc taccacctgt
    10741 actacggcgc ctcggccggc tcctaccagt tctccatggt gggcggcgag cgctcgccgc
    10801 cgcgcatcct gccgccctgc accaacgcct ccaccggctc cgcgctgctc aaccccagcc
    10861 tcccg
//
