LOCUS       sequence-336091-       10857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of RUNX1 K90N mutant protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10857)
  AUTHORS   Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, 
            Hutter S, Haferlach T, Ideker T, Mali P, Carter H
  TITLE     Interface-guided phenotyping of coding variants in the transcription
            factor RUNX1.
  JOURNAL   Cell Rep. 2024 Jul 4;43(7):114436. doi: 
            10.1016/j.celrep.2024.114436.
  PUBMED    38968069
REFERENCE   2  (bases 1 to 10857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-4"; volume: "43(7):114436. doi"; pages: " 
            10.1016/j.celrep.2024.114436"
COMMENT     Sequence Label: EF1A_Hygro_P2A_RUNX1_K90N_Barcode
FEATURES             Location/Qualifiers
     source          1..10857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        115..162
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             181..555
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    685..806
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(722..741)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     776..795
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(855..871)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(855..871)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(868..890)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    879..895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(903..909,910..927,928..933))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    948..969
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1086..1103)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1257..1845)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1337..1356)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2016..2807,2808..2876))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2639..2658
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2877..2981)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3056..3075)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3247..3626
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3628..3826
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3781..3801
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             3844..4024
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4071..4196
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4689..4922
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5107..5151
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5449..5566
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5702..6880
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5933..6871
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6828..6848
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     6897..6916
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6897..6915
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6979..8001
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6979..8001
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8011..8067
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(9613..9646)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             9671..9851
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9877..9894)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9883..10107
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10153..10581
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10240..10259)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10450..10471
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10590..10610)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(10595..10857,1..67)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(10775..10857,1..53)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
       61 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac
      121 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
      181 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
      241 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
      301 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
      361 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
      421 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
      481 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
      541 gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
      601 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
      661 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
      721 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
      781 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
      841 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
      901 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      961 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     1021 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     1081 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     1141 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     1201 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     1261 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     1321 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1381 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1441 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1501 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1561 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1621 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     1681 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1741 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1801 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1861 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1921 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1981 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     2041 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     2101 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     2161 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     2221 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     2281 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     2341 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     2401 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     2461 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     2521 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     2581 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     2641 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     2701 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     2761 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     2821 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     2881 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     2941 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     3001 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
     3061 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
     3121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
     3181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
     3241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
     3301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     3361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     3421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     3481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     3541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     3601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     3661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     3721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     3781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
     3841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     3901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     3961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     4021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     4081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     4141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     4201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     4261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     4321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     4381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     4441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     4501 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     4561 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     4621 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     4681 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     4741 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     4801 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     4861 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     4921 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     4981 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     5041 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     5101 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     5161 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     5221 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     5281 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     5341 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     5401 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     5461 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     5521 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     5581 ggacagcaga gatccagttt ggttaattaa tcgataagct ttgcaaagat ggataaagtt
     5641 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
     5701 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     5761 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
     5821 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     5881 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
     5941 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
     6001 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
     6061 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
     6121 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
     6181 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
     6241 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
     6301 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
     6361 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
     6421 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
     6481 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
     6541 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
     6601 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
     6661 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     6721 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     6781 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     6841 agtggttcaa agtttttttc ttccatttca ggtgtcgtga ggaattagct tggtactaat
     6901 acgactcact atagggagac ccaagctggc taggtaagct tgatatcgaa ttcctgcagc
     6961 ccgggggatc ccgccaccat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt
     7021 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
     7081 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
     7141 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt
     7201 ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt
     7261 gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg
     7321 gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc
     7381 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt
     7441 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc
     7501 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
     7561 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
     7621 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
     7681 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg
     7741 gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc
     7801 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac
     7861 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
     7921 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
     7981 actcgtccga gggcaaagga aggatccggc gcaacaaact tctctctgct gaaacaagcc
     8041 ggagatgtcg aagagaatcc tggaccgatg cgtatccccg tagatgccag cacgagccgc
     8101 cgcttcacgc cgccttccac cgcgctgagc ccaggcaaga tgagcgaggc gttgccgctg
     8161 ggcgccccgg acgccggcgc tgccctggcc ggcaagctga ggagcggcga ccgcagcatg
     8221 gtggaggtgc tggccgacca cccgggcgag ctggtgcgca ccgacagccc caacttcctc
     8281 tgctccgtgc tgcctacgca ctggcgctgc aacaagaccc tgcccatcgc tttcaatgtg
     8341 gtggccctag gggatgttcc agatggcact ctggtcactg tgatggctgg caatgatgaa
     8401 aactactcgg ctgagctgag aaatgctacc gcagccatga agaaccaggt tgcaagattt
     8461 aatgacctca ggtttgtcgg tcgaagtgga agagggaaaa gcttcactct gaccatcact
     8521 gtcttcacaa acccaccgca agtcgccacc taccacagag ccatcaaaat cacagtggat
     8581 gggccccgag aacctcgaag acatcggcag aaactagatg atcagaccaa gcccgggagc
     8641 ttgtcctttt ccgagcggct cagtgaactg gagcagctgc ggcgcacagc catgagggtc
     8701 agcccacacc acccagcccc cacgcccaac cctcgtgcct ccctgaacca ctccactgcc
     8761 tttaaccctc agcctcagag tcagatgcag gatacaaggc agatccaacc atccccaccg
     8821 tggtcctacg atcagtccta ccaatacctg ggatccattg cctctccttc tgtgcaccca
     8881 gcaacgccca tttcacctgg acgtgccagc ggcatgacaa ccctctctgc agaactttcc
     8941 agtcgactct caacggcacc cgacctgaca gcgttcagcg acccgcgcca gttccccgcg
     9001 ctgccctcca tctccgaccc ccgcatgcac tatccaggcg ccttcaccta ctccccgacg
     9061 ccggtcacct cgggcatcgg catcggcatg tcggccatgg gctcggccac gcgctaccac
     9121 acctacctgc cgccgcccta ccccggctcg tcgcaagcgc agggaggccc gttccaagcc
     9181 agctcgccct cctaccacct gtactacggc gcctcggccg gctcctacca gttctccatg
     9241 gtgggcggcg agcgctcgcc gccgcgcatc ctgccgccct gcaccaacgc ctccaccggc
     9301 tccgcgctgc tcaaccccag cctcccgaac cagagcgacg tggtggaggc cgagggcagc
     9361 cacagcaact cccccaccaa catggcgccc tccgcgcgcc tggaggaggc cgtgtggagg
     9421 ccctactgac tagctaagtt agaactattt cctggctgtt acgcgcgcta ggtaggtcta
     9481 ctgacgggct tgttaaacag tgaacccacg acccgcagcg cccgaccgaa aggagcgcac
     9541 gaccccatgc atcgaacaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     9601 agacaagata tcataacttc gtatagcata cattatacga agttatcgtc tagctggtcc
     9661 ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     9721 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     9781 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     9841 aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     9901 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     9961 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
    10021 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
    10081 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
    10141 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
    10201 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
    10261 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
    10321 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
    10381 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
    10441 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
    10501 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
    10561 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
    10621 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
    10681 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
    10741 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
    10801 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggc
//
