LOCUS       sequence-336096-       10865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of RUNX1 A120D mutant protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, 
            Hutter S, Haferlach T, Ideker T, Mali P, Carter H
  TITLE     Interface-guided phenotyping of coding variants in the transcription
            factor RUNX1.
  JOURNAL   Cell Rep. 2024 Jul 4;43(7):114436. doi: 
            10.1016/j.celrep.2024.114436.
  PUBMED    38968069
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-4"; volume: "43(7):114436. doi"; pages: " 
            10.1016/j.celrep.2024.114436"
COMMENT     Sequence Label: EF1A_Hygro_P2A_RUNX1_A120D_Barcode
FEATURES             Location/Qualifiers
     source          1..10865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(134..722)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(214..233)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(893..1684,1685..1753))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1516..1535
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1754..1858)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1933..1952)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2124..2503
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2703
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2658..2678
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2721..2901
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2948..3073
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3566..3799
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3984..4028
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4326..4443
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4579..5757
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4810..5748
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5705..5725
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     5774..5793
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5774..5792
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5856..6878
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5856..6878
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6888..6944
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(8498..8531)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             8556..8736
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8762..8779)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8768..8992
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9038..9466
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9125..9144)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9335..9356
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9475..9495)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9480..9809
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9660..9795
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        9857..9904
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9923..10297
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10427..10548
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10464..10483)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10518..10537
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10597..10613)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10597..10613)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10610..10632)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10621..10637
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10645..10651,10652..10669,10670..10675))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10690..10711
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10828..10845)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
       61 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      121 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      181 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      241 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      301 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      361 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      421 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      481 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      541 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
      601 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      661 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      721 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
      781 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
      841 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
      901 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
      961 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     1021 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     1081 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     1141 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     1201 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     1261 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     1321 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     1381 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     1441 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     1501 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     1561 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     1621 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     1681 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     1741 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     1801 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     1861 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     1921 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     1981 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     2041 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
     2101 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
     2161 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     2221 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     2281 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     2341 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     2401 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     2461 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     2521 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     2581 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     2641 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     2701 cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     2761 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     2821 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     2881 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     2941 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     3001 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     3061 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     3121 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     3181 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     3241 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     3301 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     3361 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     3421 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     3481 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     3541 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     3601 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     3661 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     3721 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     3781 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     3841 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     3901 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     3961 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     4021 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     4081 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     4141 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     4201 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     4261 gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc
     4321 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     4381 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     4441 tttcgggttt attacaggga cagcagagat ccagtttggt taattaatcg ataagctttg
     4501 caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc
     4561 ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     4621 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
     4681 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     4741 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     4801 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc
     4861 ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt
     4921 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     4981 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     5041 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     5101 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     5161 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     5221 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     5281 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     5341 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     5401 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     5461 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     5521 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     5581 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     5641 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     5701 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga
     5761 attagcttgg tactaatacg actcactata gggagaccca agctggctag gtaagcttga
     5821 tatcgaattc ctgcagcccg ggggatcccg ccaccatgaa aaagcctgaa ctcaccgcga
     5881 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct
     5941 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc
     6001 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat
     6061 cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct
     6121 attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc
     6181 ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc
     6241 agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg
     6301 atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca
     6361 ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc
     6421 ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg
     6481 gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg
     6541 tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact
     6601 tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca
     6661 ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg
     6721 cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa
     6781 tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg
     6841 gaaaccgacg ccccagcact cgtccgaggg caaaggaagg atccggcgca acaaacttct
     6901 ctctgctgaa acaagccgga gatgtcgaag agaatcctgg accgatgcgt atccccgtag
     6961 atgccagcac gagccgccgc ttcacgccgc cttccaccgc gctgagccca ggcaagatga
     7021 gcgaggcgtt gccgctgggc gccccggacg ccggcgctgc cctggccggc aagctgagga
     7081 gcggcgaccg cagcatggtg gaggtgctgg ccgaccaccc gggcgagctg gtgcgcaccg
     7141 acagccccaa cttcctctgc tccgtgctgc ctacgcactg gcgctgcaac aagaccctgc
     7201 ccatcgcttt caaggtggtg gccctagggg atgttccaga tggcactctg gtcactgtga
     7261 tggctggcaa tgatgaaaac tactcggctg agctgagaaa tgataccgca gccatgaaga
     7321 accaggttgc aagatttaat gacctcaggt ttgtcggtcg aagtggaaga gggaaaagct
     7381 tcactctgac catcactgtc ttcacaaacc caccgcaagt cgccacctac cacagagcca
     7441 tcaaaatcac agtggatggg ccccgagaac ctcgaagaca tcggcagaaa ctagatgatc
     7501 agaccaagcc cgggagcttg tccttttccg agcggctcag tgaactggag cagctgcggc
     7561 gcacagccat gagggtcagc ccacaccacc cagcccccac gcccaaccct cgtgcctccc
     7621 tgaaccactc cactgccttt aaccctcagc ctcagagtca gatgcaggat acaaggcaga
     7681 tccaaccatc cccaccgtgg tcctacgatc agtcctacca atacctggga tccattgcct
     7741 ctccttctgt gcacccagca acgcccattt cacctggacg tgccagcggc atgacaaccc
     7801 tctctgcaga actttccagt cgactctcaa cggcacccga cctgacagcg ttcagcgacc
     7861 cgcgccagtt ccccgcgctg ccctccatct ccgacccccg catgcactat ccaggcgcct
     7921 tcacctactc cccgacgccg gtcacctcgg gcatcggcat cggcatgtcg gccatgggct
     7981 cggccacgcg ctaccacacc tacctgccgc cgccctaccc cggctcgtcg caagcgcagg
     8041 gaggcccgtt ccaagccagc tcgccctcct accacctgta ctacggcgcc tcggccggct
     8101 cctaccagtt ctccatggtg ggcggcgagc gctcgccgcc gcgcatcctg ccgccctgca
     8161 ccaacgcctc caccggctcc gcgctgctca accccagcct cccgaaccag agcgacgtgg
     8221 tggaggccga gggcagccac agcaactccc ccaccaacat ggcgccctcc gcgcgcctgg
     8281 aggaggccgt gtggaggccc tactgactag ctaagttaga actatttcct ggctgttacg
     8341 cgcttggcga actactactg acgggcttgt taaacagtga accgcccgcc ccacgacccg
     8401 cagcgcccga ccgaaaggag cgcacgaccc catgcatcga acaaaagaaa aggggggact
     8461 ggaagggcta attcactccc aacgaagaca agatatcata acttcgtata gcatacatta
     8521 tacgaagtta tcgtctagct ggtccggact gtactgggtc tctctggtta gaccagatct
     8581 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     8641 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     8701 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca
     8761 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     8821 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     8881 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     8941 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     9001 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     9061 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     9121 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     9181 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     9241 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     9301 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     9361 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     9421 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     9481 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     9541 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     9601 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     9661 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     9721 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     9781 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     9841 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     9901 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
     9961 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
    10021 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
    10081 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
    10141 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
    10201 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
    10261 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
    10321 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
    10381 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
    10441 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    10501 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
    10561 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
    10621 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
    10681 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
    10741 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
    10801 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    10861 gctgc
//
