LOCUS       sequence-336097-        9320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of 3xFlag-tagged human androgen receptor 
            phosphorylation mutant: AR-Ser650Asp (AR-S650D) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9320)
  AUTHORS   Liu X, Han W, Gulla S, Simon NI, Gao Y, Cai C, Yang H, Zhang X, Liu 
            J, Balk SP, Chen S
  TITLE     Protein phosphatase 1 suppresses androgen receptor ubiquitylation 
            and degradation.
  JOURNAL   Oncotarget. 2016 Jan 12;7(2):1754-64. doi: 10.18632/oncotarget.6434.
  PUBMED    26636645
REFERENCE   2  (bases 1 to 9320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.18632/oncotarget"; journalName: "Oncotarget"; date: "2016-01-12-
            12"; volume: "7"; issue: "2"; pages: "1754-64"
COMMENT     Sequence Label: Flag-M4-AR-S650D
FEATURES             Location/Qualifiers
     source          1..9320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..148
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     140..157
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     852..872
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             931..996
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    4079..4701
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4278..4297)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(4725..4745)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4743..5040
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5094..5888
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5148..5167)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5758..5777
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6540..6674
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6577..6596)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6631..6650
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6679..6698)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6680..6698)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6712..6728)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6712..6728)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6725..6747)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6736..6752
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6760..6766,6767..6784,6785..6790))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6805..6826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6943..6960)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7114..7702)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7194..7213)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7873..8664,8665..8733))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8496..8515
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8734..8838)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8865..9293
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8952..8971)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9162..9183
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attaaccatg gactacaaag accatgacgg tgattataaa
      961 gatcatgaca tcgattacaa ggatgacgat gacaagcttc cgcggggatc catggaagtg
     1021 cagttagggc tgggaagggt ctaccctcgg ccgccgtcca agacctaccg aggagctttc
     1081 cagaatctgt tccagagcgt gcgcgaagtg atccagaacc cgggccccag gcacccagag
     1141 gccgcgagcg cagcacctcc cggcgccagt ttgctgctgc tgcagcagca gcagcagcag
     1201 cagcagcagc agcagcagca gcagcagcag cagcagcagc agcaagagac tagccccagg
     1261 cagcagcagc agcagcaggg tgaggatggt tctccccaag cccatcgtag aggccccaca
     1321 ggctacctgg tcctggatga ggaacagcaa ccttcacagc cgcagtcggc cctggagtgc
     1381 caccccgaga gaggttgcgt cccagagcct ggagccgccg tggccgccag caaggggctg
     1441 ccgcagcagc tgccagcacc tccggacgag gatgactcag ctgccccatc cacgttgtcc
     1501 ctgctgggcc ccactttccc cggcttaagc agctgctccg ctgaccttaa agacatcctg
     1561 agcgaggcca gcaccatgca actccttcag caacagcagc aggaagcagt atccgaaggc
     1621 agcagcagcg ggagagcgag ggaggcctcg ggggctccca cttcctccaa ggacaattac
     1681 ttagggggca cttcgaccat ttctgacaac gccaaggagt tgtgtaaggc agtgtcggtg
     1741 tccatgggcc tgggtgtgga ggcgttggag catctgagtc caggggaaca gcttcggggg
     1801 gattgcatgt acgccccact tttgggagtt ccacccgctg tgcgtcccac tccttgtgcc
     1861 ccattggccg aatgcaaagg ttctctgcta gacgacagcg caggcaagag cactgaagat
     1921 actgctgagt attccccttt caagggaggt tacaccaaag ggctagaagg cgagagccta
     1981 ggctgctctg gcagcgctgc agcagggagc tccgggacac ttgaactgcc gtctaccctg
     2041 tctctctaca agtccggagc actggacgag gcagctgcgt accagagtcg cgactactac
     2101 aactttccac tggctctggc cggaccgccg ccccctccgc cgcctcccca tccccacgct
     2161 cgcatcaagc tggagaaccc gctggactac ggcagcgcct gggcggctgc ggcggcgcag
     2221 tgccgctatg gggacctggc gagcctgcat ggcgcgggtg cagcgggacc cggttctggg
     2281 tcaccctcag ccgccgcttc ctcatcctgg cacactctct tcacagccga agaaggccag
     2341 ttgtatggac cgtgtggtgg tggtgggggt ggtggcggcg gcggcggcgg cggcggcggc
     2401 ggcggcggcg gcggcggcgg cggcgaggcg ggagctgtag ccccctacgg ctacactcgg
     2461 ccccctcagg ggctggcggg ccaggaaagc gacttcaccg cacctgatgt gtggtaccct
     2521 ggcggcatgg tgagcagagt gccctatccc agtcccactt gtgtcaaaag cgaaatgggc
     2581 ccctggatgg atagctactc cggaccttac ggggacatgc gtttggagac tgccagggac
     2641 catgttttgc ccattgacta ttactttcca ccccagaaga cctgcctgat ctgtggagat
     2701 gaagcttctg ggtgtcacta tggagctctc acatgtggaa gctgcaaggt cttcttcaaa
     2761 agagccgctg aagggaaaca gaagtacctg tgcgccagca gaaatgattg cactattgat
     2821 aaattccgaa ggaaaaattg tccatcttgt cgtcttcgga aatgttatga agcagggatg
     2881 actctgggag cccggaagct gaagaaactt ggtaatctga aactacagga ggaaggagag
     2941 gcttccagca ccaccgaccc cactgaggag acaacccaga agctgacagt gtcacacatt
     3001 gaaggctatg aatgtcagcc catctttctg aatgtcctgg aagccattga gccaggtgta
     3061 gtgtgtgctg gacacgacaa caaccagccc gactcctttg cagccttgct ctctagcctc
     3121 aatgaactgg gagagagaca gcttgtacac gtggtcaagt gggccaaggc cttgcctggc
     3181 ttccgcaact tacacgtgga cgaccagatg gctgtcattc agtactcctg gatggggctc
     3241 atggtgtttg ccatgggctg gcgatccttc accaatgtca actccaggat gctctacttc
     3301 gcccctgatc tggttttcaa tgagtaccgc atgcacaagt cccggatgta cagccagtgt
     3361 gtccgaatga ggcacctctc tcaagagttt ggatggctcc aaatcacccc ccaggaattc
     3421 ctgtgcatga aagcactgct actcttcagc attattccag tggatgggct gaaaaatcaa
     3481 aaattctttg atgaacttcg aatgaactac atcaaggaac tcgatcgtat cattgcatgc
     3541 aaaagaaaaa atcccacatc ctgctcaaga cgcttctacc agctcaccaa gctcctggac
     3601 tccgtgcagc ctattgcgag agagctgcat cagttcactt ttgacctgct aatcaagtca
     3661 cacatggtga gcgtggactt tccggaaatg atggcagaga tcatctctgt gcaagtgccc
     3721 aagatccttt ctgggaaagt caagcccatc tatttccaca cccagtgaag cattggaaac
     3781 cctatttccc caccccagct catgccccct ttcagatgtc ttctgcctgt tataactctg
     3841 cactactcct ctgcagtgcc ttggggaatt tcctctattg atgtacagtc tgtcatgaac
     3901 atgttcctca attctatttg ctgggctttt tttttctctt tctctccttt ctttttcttc
     3961 ttccctccct atctaaccct cccatggcac cttcagactt tgcttcccat tgtggctcct
     4021 atctgtgttt tgaatggtgt tgtatgcctt tatccagcac agtggcggcc gcgattccgg
     4081 gtggcatccc tgtgacccct ccccagtgcc tctcctggcc ctggaagttg ccactccagt
     4141 gcccaccagc cttgtcctaa taaaattaag ttgcatcatt ttgtctgact aggtgtcctt
     4201 ctataatatt atggggtgga ggggggtggt atggagcaag gggcaagttg ggaagacaac
     4261 ctgtagggcc tgcggggtct attgggaacc aagctggagt gcagtggcac aatcttggct
     4321 cactgcaatc tccgcctcct gggttcaagc gattctcctg cctcagcctc ccgagttgtt
     4381 gggattccag gcatgcatga ccaggctcag ctaatttttg tttttttggt agagacgggg
     4441 tttcaccata ttggccaggc tggtctccaa ctcctaatct caggtgatct acccaccttg
     4501 gcctcccaaa ttgctgggat tacaggcgtg aaccactgct cccttccctg tccttctgat
     4561 tttaaaataa ctataccagc aggaggacgt ccagacacag cataggctac ctggccatgc
     4621 ccaaccggtg ggacatttga gttgcttgct tggcactgtc ctctcatgcg ttgggtccac
     4681 tcagtagatg cctgttgaat tgggtacgcg gccagcttgg ctgtggaatg tgtgtcagtt
     4741 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     4801 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4861 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     4921 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4981 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     5041 aggaattgat cagcttggga tctgatcaag agacaggatg aggatcgttt cgcatgattg
     5101 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     5161 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     5221 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     5281 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     5341 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     5401 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     5461 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     5521 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     5581 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     5641 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     5701 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     5761 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     5821 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     5881 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     5941 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     6001 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccacct
     6061 cgggctcgat cccctcgcga gttggttcag ctgctgcctg aggctggacg acctcgcgga
     6121 gttctaccgg cagtgcaaat ccgtcggcat ccaggaaacc agcagcggct atccgcgcat
     6181 ccatgccccc gaactgcagg agtggggagg cacgatggcc gctttggtcg acccggacgg
     6241 gacgctcctg cgcctgatac agaacgaatt gcttgcaggc atctcatgag tgtgtcttcc
     6301 cgttttccgc ctgaggtcac tgcgtggatg gagcgctggc gcctgctgcg cgacggcgag
     6361 ctgctcacca cccactcgcc aagctggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6421 cataggctcc gccgatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     6481 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     6541 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     6601 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     6661 atcatgtctg gatcaattcc ctatagtgag tcgtattaaa ttcgtaatca tgtcatagct
     6721 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     6781 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     6841 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     6901 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     6961 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     7021 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     7081 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     7141 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     7201 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     7261 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     7321 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     7381 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     7441 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     7501 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     7561 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7621 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7681 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7741 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7801 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7861 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7921 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7981 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     8041 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     8101 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     8161 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     8221 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     8281 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     8341 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     8401 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     8461 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     8521 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     8581 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     8641 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     8701 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     8761 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     8821 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg
     8881 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     8941 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     9001 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     9061 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     9121 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     9181 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     9241 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     9301 aatattaacg cttacaattt
//
