LOCUS       sequence-336108-       10864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of RUNX1 V74E mutant protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10864)
  AUTHORS   Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, 
            Hutter S, Haferlach T, Ideker T, Mali P, Carter H
  TITLE     Interface-guided phenotyping of coding variants in the transcription
            factor RUNX1.
  JOURNAL   Cell Rep. 2024 Jul 4;43(7):114436. doi: 
            10.1016/j.celrep.2024.114436.
  PUBMED    38968069
REFERENCE   2  (bases 1 to 10864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-4"; volume: "43(7):114436. doi"; pages: " 
            10.1016/j.celrep.2024.114436"
COMMENT     Sequence Label: EF1A_Hygro_P2A_RUNX1_V74E_Barcode
FEATURES             Location/Qualifiers
     source          1..10864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(82..102)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        87..416
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      267..402
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        464..511
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             530..904
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1034..1155
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1071..1090)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1125..1144
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1204..1220)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1204..1220)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1217..1239)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1228..1244
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1252..1258,1259..1276,1277..1282))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1297..1318
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1435..1452)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1606..2194)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1686..1705)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2365..3156,3157..3225))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2988..3007
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3226..3330)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3405..3424)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3596..3975
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3977..4175
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4130..4150
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4193..4373
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4420..4545
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5038..5271
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5456..5500
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5798..5915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6051..7229
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6282..7220
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7177..7197
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     7246..7265
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7246..7264
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7328..8350
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7328..8350
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8360..8416
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(9969..10002)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             10027..10207
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10233..10250)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10239..10463
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(10509..10864,1..73)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10596..10615)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10806..10827
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
       61 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
      121 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
      181 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
      241 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
      301 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
      361 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
      421 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc
      481 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt
      541 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac
      601 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga
      661 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc
      721 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac
      781 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg
      841 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga
      901 ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
      961 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     1021 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     1081 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     1141 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     1201 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     1261 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     1321 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     1381 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     1441 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     1501 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     1561 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1621 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1681 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1741 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1801 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1861 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1921 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1981 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2041 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2101 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2161 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     2221 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     2281 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     2341 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     2401 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     2461 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     2521 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     2581 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     2641 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     2701 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     2761 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     2821 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     2881 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     2941 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     3001 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     3061 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     3121 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     3181 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     3241 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     3301 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     3361 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
     3421 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     3481 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
     3541 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
     3601 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     3661 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     3721 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     3781 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     3841 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     3901 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     3961 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     4021 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     4081 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     4141 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct
     4201 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     4261 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     4321 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     4381 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     4441 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     4501 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     4561 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     4621 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     4681 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     4741 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     4801 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     4861 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     4921 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     4981 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     5041 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     5101 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     5161 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     5221 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     5281 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     5341 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     5401 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     5461 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     5521 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     5581 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     5641 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     5701 aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc
     5761 gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     5821 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     5881 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     5941 atccagtttg gttaattaat cgataagctt tgcaaagatg gataaagttt taaacagaga
     6001 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
     6061 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     6121 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     6181 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     6241 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
     6301 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
     6361 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
     6421 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
     6481 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
     6541 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
     6601 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
     6661 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
     6721 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
     6781 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
     6841 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
     6901 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
     6961 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
     7021 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     7081 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
     7141 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
     7201 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta
     7261 tagggagacc caagctggct aggtaagctt gatatcgaat tcctgcagcc cgggggatcc
     7321 cgccaccatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa
     7381 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag
     7441 cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta
     7501 caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct
     7561 tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt
     7621 cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc
     7681 catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc
     7741 gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca
     7801 tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct
     7861 cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga
     7921 tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag
     7981 cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg
     8041 gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg
     8101 atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt
     8161 ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg
     8221 atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac
     8281 cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag
     8341 ggcaaaggaa ggatccggcg caacaaactt ctctctgctg aaacaagccg gagatgtcga
     8401 agagaatcct ggaccgctgc gtatccccgt agatgccagc acgagccgcc gcttcacgcc
     8461 gccttccacc gcgctgagcc caggcaagat gagcgaggcg ttgccgctgg gcgccccgga
     8521 cgccggcgct gccctggccg gcaagctgag gagcggcgac cgcagcatgg tggaggtgct
     8581 ggccgaccac ccgggcgagc tggtgcgcac cgacagcccc aacttcctct gctccgagct
     8641 gcctacgcac tggcgctgca acaagaccct gcccatcgct ttcaaggtgg tggccctagg
     8701 ggatgttcca gatggcactc tggtcactgt gatggctggc aatgatgaaa actactcggc
     8761 tgagctgaga aatgctaccg cagccatgaa gaaccaggtt gcaagattta atgacctcag
     8821 gtttgtcggt cgaagtggaa gagggaaaag cttcactctg accatcactg tcttcacaaa
     8881 cccaccgcaa gtcgccacct accacagagc catcaaaatc acagtggatg ggccccgaga
     8941 acctcgaaga catcggcaga aactagatga tcagaccaag cccgggagct tgtccttttc
     9001 cgagcggctc agtgaactgg agcagctgcg gcgcacagcc atgagggtca gcccacacca
     9061 cccagccccc acgcccaacc ctcgtgcctc cctgaaccac tccactgcct ttaaccctca
     9121 gcctcagagt cagatgcagg atacaaggca gatccaacca tccccaccgt ggtcctacga
     9181 tcagtcctac caatacctgg gatccattgc ctctccttct gtgcacccag caacgcccat
     9241 ttcacctgga cgtgccagcg gcatgacaac cctctctgca gaactttcca gtcgactctc
     9301 aacggcaccc gacctgacag cgttcagcga cccgcgccag ttccccgcgc tgccctccat
     9361 ctccgacccc cgcatgcact atccaggcgc cttcacctac tccccgacgc cggtcacctc
     9421 gggcatcggc atcggcatgt cggccatggg ctcggccacg cgctaccaca cctacctgcc
     9481 gccgccctac cccggctcgt cgcaagcgca gggaggcccg ttccaagcca gctcgccctc
     9541 ctaccacctg tactacggcg cctcggccgg ctcctaccag ttctccatgg tgggcggcga
     9601 gcgctcgccg ccgcgcatcc tgccgccctg caccaacgcc tccaccggct ccgcgctgct
     9661 caaccccagc ctcccgaacc agagcgacgt ggtggaggcc gagggcagcc acagcaactc
     9721 ccccaccaac atggcgccct ccgcgcgcct ggaggaggcc gtgtggaggc cctactgact
     9781 agctaagtta gaactatttc ctggctgtac gcgagctatg cgttactact gacgggcttg
     9841 ttaaacagtg aaccgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc
     9901 ccatgcatcg aacaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     9961 aagatatcat aacttcgtat agcatacatt atacgaagtt atcgtctagc tggtccggac
    10021 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
    10081 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
    10141 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
    10201 tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
    10261 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
    10321 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
    10381 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
    10441 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
    10501 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
    10561 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
    10621 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
    10681 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
    10741 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
    10801 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
    10861 tgat
//
