LOCUS       sequence-336113-       10865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of RUNX1 N69Y mutant protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, 
            Hutter S, Haferlach T, Ideker T, Mali P, Carter H
  TITLE     Interface-guided phenotyping of coding variants in the transcription
            factor RUNX1.
  JOURNAL   Cell Rep. 2024 Jul 4;43(7):114436. doi: 
            10.1016/j.celrep.2024.114436.
  PUBMED    38968069
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-4"; volume: "43(7):114436. doi"; pages: " 
            10.1016/j.celrep.2024.114436"
COMMENT     Sequence Label: EF1A_Hygro_P2A_RUNX1_N69Y_Barcode
FEATURES             Location/Qualifiers
     source          1..10865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    618..851
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1036..1080
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1378..1495
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1631..2809
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1862..2800
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2757..2777
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     2826..2845
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2826..2844
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2908..3930
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2908..3930
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3940..3996
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(5550..5583)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             5608..5788
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5814..5831)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5820..6044
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6090..6518
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6177..6196)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6387..6408
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6527..6547)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6532..6861
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6712..6847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6909..6956
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6975..7349
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7479..7600
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7516..7535)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7570..7589
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7649..7665)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7649..7665)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7662..7684)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7673..7689
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7697..7703,7704..7721,7722..7727))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7742..7763
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7880..7897)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8051..8639)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8131..8150)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8810..9601,9602..9670))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9433..9452
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9671..9775)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9850..9869)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10041..10420
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10422..10620
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10575..10595
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10638..10818
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    join(10865,1..125)
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
       61 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
      121 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
      181 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
      241 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
      301 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
      361 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
      421 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
      481 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
      541 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
      601 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
      661 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
      721 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
      781 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
      841 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
      901 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
      961 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     1021 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     1081 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     1141 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     1201 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     1261 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     1321 aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt
     1381 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1441 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     1501 ttattacagg gacagcagag atccagtttg gttaattaat cgataagctt tgcaaagatg
     1561 gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg
     1621 agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     1681 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa
     1741 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     1801 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     1861 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
     1921 gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg
     1981 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt
     2041 tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg
     2101 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct
     2161 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt
     2221 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg
     2281 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc
     2341 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg
     2401 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc
     2461 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag
     2521 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag
     2581 gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt
     2641 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca
     2701 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa
     2761 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt
     2821 ggtactaata cgactcacta tagggagacc caagctggct aggtaagctt gatatcgaat
     2881 tcctgcagcc cgggggatcc cgccaccatg aaaaagcctg aactcaccgc gacgtctgtc
     2941 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc
     3001 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat
     3061 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg
     3121 ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc
     3181 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt
     3241 ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc
     3301 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata
     3361 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt
     3421 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc
     3481 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata
     3541 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac
     3601 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg
     3661 aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt
     3721 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt
     3781 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc
     3841 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga
     3901 cgccccagca ctcgtccgag ggcaaaggaa ggatccggcg caacaaactt ctctctgctg
     3961 aaacaagccg gagatgtcga agagaatcct ggaccgatgc gtatccccgt agatgccagc
     4021 acgagccgcc gcttcacgcc gccttccacc gcgctgagcc caggcaagat gagcgaggcg
     4081 ttgccgctgg gcgccccgga cgccggcgct gccctggccg gcaagctgag gagcggcgac
     4141 cgcagcatgg tggaggtgct ggccgaccac ccgggcgagc tggtgcgcac cgacagcccc
     4201 tacttcctct gctccgtgct gcctacgcac tggcgctgca acaagaccct gcccatcgct
     4261 ttcaaggtgg tggccctagg ggatgttcca gatggcactc tggtcactgt gatggctggc
     4321 aatgatgaaa actactcggc tgagctgaga aatgctaccg cagccatgaa gaaccaggtt
     4381 gcaagattta atgacctcag gtttgtcggt cgaagtggaa gagggaaaag cttcactctg
     4441 accatcactg tcttcacaaa cccaccgcaa gtcgccacct accacagagc catcaaaatc
     4501 acagtggatg ggccccgaga acctcgaaga catcggcaga aactagatga tcagaccaag
     4561 cccgggagct tgtccttttc cgagcggctc agtgaactgg agcagctgcg gcgcacagcc
     4621 atgagggtca gcccacacca cccagccccc acgcccaacc ctcgtgcctc cctgaaccac
     4681 tccactgcct ttaaccctca gcctcagagt cagatgcagg atacaaggca gatccaacca
     4741 tccccaccgt ggtcctacga tcagtcctac caatacctgg gatccattgc ctctccttct
     4801 gtgcacccag caacgcccat ttcacctgga cgtgccagcg gcatgacaac cctctctgca
     4861 gaactttcca gtcgactctc aacggcaccc gacctgacag cgttcagcga cccgcgccag
     4921 ttccccgcgc tgccctccat ctccgacccc cgcatgcact atccaggcgc cttcacctac
     4981 tccccgacgc cggtcacctc gggcatcggc atcggcatgt cggccatggg ctcggccacg
     5041 cgctaccaca cctacctgcc gccgccctac cccggctcgt cgcaagcgca gggaggcccg
     5101 ttccaagcca gctcgccctc ctaccacctg tactacggcg cctcggccgg ctcctaccag
     5161 ttctccatgg tgggcggcga gcgctcgccg ccgcgcatcc tgccgccctg caccaacgcc
     5221 tccaccggct ccgcgctgct caaccccagc ctcccgaacc agagcgacgt ggtggaggcc
     5281 gagggcagcc acagcaactc ccccaccaac atggcgccct ccgcgcgcct ggaggaggcc
     5341 gtgtggaggc cctactgact agctaagtta gaactatttc ctggctgtta cgcgaatgac
     5401 caattactac tgacgggctt gttaaacagt gaaccgcccg ccccacgacc cgcagcgccc
     5461 gaccgaaagg agcgcacgac cccatgcatc gaacaaaaga aaagggggga ctggaagggc
     5521 taattcactc ccaacgaaga caagatatca taacttcgta tagcatacat tatacgaagt
     5581 tatcgtctag ctggtccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg
     5641 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     5701 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     5761 ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac
     5821 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     5881 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     5941 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     6001 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     6061 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     6121 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     6181 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     6241 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     6301 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     6361 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     6421 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     6481 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     6541 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     6601 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6661 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     6721 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6781 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     6841 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     6901 cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
     6961 tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg
     7021 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg
     7081 acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc
     7141 aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg
     7201 ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg
     7261 agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg
     7321 tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg
     7381 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     7441 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     7501 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7561 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     7621 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7681 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7741 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7801 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7861 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7921 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7981 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8041 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8101 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8161 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8221 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8281 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8341 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8401 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8461 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     8521 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8581 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8641 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8701 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8761 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8821 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8881 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8941 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9001 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9061 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9121 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9181 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9241 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9301 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9361 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9421 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9481 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9541 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9601 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9661 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9721 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9781 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
     9841 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
     9901 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     9961 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
    10021 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
    10081 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
    10141 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
    10201 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
    10261 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
    10321 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
    10381 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
    10441 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
    10501 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
    10561 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
    10621 cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
    10681 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
    10741 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
    10801 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
    10861 ggagc
//
