LOCUS       sequence-336114-       10865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of RUNX1 R118G mutant protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, 
            Hutter S, Haferlach T, Ideker T, Mali P, Carter H
  TITLE     Interface-guided phenotyping of coding variants in the transcription
            factor RUNX1.
  JOURNAL   Cell Rep. 2024 Jul 4;43(7):114436. doi: 
            10.1016/j.celrep.2024.114436.
  PUBMED    38968069
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-4"; volume: "43(7):114436. doi"; pages: " 
            10.1016/j.celrep.2024.114436"
COMMENT     Sequence Label: EF1A_Hygro_P2A_RUNX1_R118G_Barcode
FEATURES             Location/Qualifiers
     source          1..10865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        127..174
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             193..567
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    697..818
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(734..753)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     788..807
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(867..883)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(867..883)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(880..902)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    891..907
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(915..921,922..939,940..945))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    960..981
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1098..1115)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1269..1857)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1349..1368)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2028..2819,2820..2888))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2651..2670
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2889..2993)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3068..3087)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3259..3638
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3640..3838
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3793..3813
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             3856..4036
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4083..4208
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4701..4934
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5119..5163
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5461..5578
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5714..6892
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5945..6883
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6840..6860
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     6909..6928
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6909..6927
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6991..8013
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6991..8013
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8023..8079
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(9633..9666)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             9691..9871
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9897..9914)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9903..10127
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10173..10601
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10260..10279)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10470..10491
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10610..10630)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(10615..10865,1..79)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(10795..10865,1..65)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
       61 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
      121 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
      181 aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
      241 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
      301 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
      361 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
      421 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
      481 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
      541 cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc
      601 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
      661 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
      721 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
      781 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
      841 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      901 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
      961 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     1021 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     1081 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     1141 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     1201 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1261 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1321 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1381 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1441 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1501 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1561 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1621 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1681 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1741 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1801 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1861 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1921 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1981 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     2041 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     2101 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     2161 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     2221 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     2281 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     2341 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2401 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2461 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2521 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2581 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2641 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     2701 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2761 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2821 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2881 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     2941 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     3001 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
     3061 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     3121 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     3181 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
     3241 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     3301 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     3361 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     3421 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     3481 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     3541 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     3601 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     3661 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     3721 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     3781 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg
     3841 cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     3901 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     3961 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     4021 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     4081 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     4141 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     4201 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     4261 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     4321 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     4381 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     4441 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     4501 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     4561 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     4621 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     4681 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     4741 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     4801 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     4861 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     4921 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     4981 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     5041 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     5101 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     5161 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     5221 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     5281 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     5341 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     5401 gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat tcatccacaa
     5461 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     5521 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     5581 ggtttattac agggacagca gagatccagt ttggttaatt aatcgataag ctttgcaaag
     5641 atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa
     5701 aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     5761 cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt
     5821 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     5881 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     5941 acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg
     6001 cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt
     6061 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg
     6121 agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc
     6181 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac
     6241 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc
     6301 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag
     6361 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc
     6421 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc
     6481 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag
     6541 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa
     6601 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc
     6661 caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg
     6721 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg
     6781 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct
     6841 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggaattag
     6901 cttggtacta atacgactca ctatagggag acccaagctg gctaggtaag cttgatatcg
     6961 aattcctgca gcccggggga tcccgccacc atgaaaaagc ctgaactcac cgcgacgtct
     7021 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
     7081 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
     7141 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
     7201 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
     7261 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     7321 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
     7381 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     7441 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     7501 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     7561 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     7621 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     7681 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     7741 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     7801 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     7861 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     7921 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     7981 cgacgcccca gcactcgtcc gagggcaaag gaaggatccg gcgcaacaaa cttctctctg
     8041 ctgaaacaag ccggagatgt cgaagagaat cctggaccga tgcgtatccc cgtagatgcc
     8101 agcacgagcc gccgcttcac gccgccttcc accgcgctga gcccaggcaa gatgagcgag
     8161 gcgttgccgc tgggcgcccc ggacgccggc gctgccctgg ccggcaagct gaggagcggc
     8221 gaccgcagca tggtggaggt gctggccgac cacccgggcg agctggtgcg caccgacagc
     8281 cccaacttcc tctgctccgt gctgcctacg cactggcgct gcaacaagac cctgcccatc
     8341 gctttcaagg tggtggccct aggggatgtt ccagatggca ctctggtcac tgtgatggct
     8401 ggcaatgatg aaaactactc ggctgagctg ggaaatgcta ccgcagccat gaagaaccag
     8461 gttgcaagat ttaatgacct caggtttgtc ggtcgaagtg gaagagggaa aagcttcact
     8521 ctgaccatca ctgtcttcac aaacccaccg caagtcgcca cctaccacag agccatcaaa
     8581 atcacagtgg atgggccccg agaacctcga agacatcggc agaaactaga tgatcagacc
     8641 aagcccggga gcttgtcctt ttccgagcgg ctcagtgaac tggagcagct gcggcgcaca
     8701 gccatgaggg tcagcccaca ccacccagcc cccacgccca accctcgtgc ctccctgaac
     8761 cactccactg cctttaaccc tcagcctcag agtcagatgc aggatacaag gcagatccaa
     8821 ccatccccac cgtggtccta cgatcagtcc taccaatacc tgggatccat tgcctctcct
     8881 tctgtgcacc cagcaacgcc catttcacct ggacgtgcca gcggcatgac aaccctctct
     8941 gcagaacttt ccagtcgact ctcaacggca cccgacctga cagcgttcag cgacccgcgc
     9001 cagttccccg cgctgccctc catctccgac ccccgcatgc actatccagg cgccttcacc
     9061 tactccccga cgccggtcac ctcgggcatc ggcatcggca tgtcggccat gggctcggcc
     9121 acgcgctacc acacctacct gccgccgccc taccccggct cgtcgcaagc gcagggaggc
     9181 ccgttccaag ccagctcgcc ctcctaccac ctgtactacg gcgcctcggc cggctcctac
     9241 cagttctcca tggtgggcgg cgagcgctcg ccgccgcgca tcctgccgcc ctgcaccaac
     9301 gcctccaccg gctccgcgct gctcaacccc agcctcccga accagagcga cgtggtggag
     9361 gccgagggca gccacagcaa ctcccccacc aacatggcgc cctccgcgcg cctggaggag
     9421 gccgtgtgga ggccctactg actagctaag ttagaactat ttcctggctg ttacgcgaat
     9481 agttatatcc tactgacggg cttgttaaac agtgaaccgc ccgccccacg acccgcagcg
     9541 cccgaccgaa aggagcgcac gaccccatgc atcgaacaaa agaaaagggg ggactggaag
     9601 ggctaattca ctcccaacga agacaagata tcataacttc gtatagcata cattatacga
     9661 agttatcgtc tagctggtcc ggactgtact gggtctctct ggttagacca gatctgagcc
     9721 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     9781 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     9841 cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc
     9901 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     9961 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
    10021 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
    10081 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
    10141 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
    10201 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
    10261 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
    10321 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
    10381 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
    10441 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
    10501 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
    10561 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
    10621 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
    10681 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
    10741 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
    10801 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
    10861 tatgc
//
