LOCUS       sequence-336117-       10865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of RUNX1 T65I mutant protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, 
            Hutter S, Haferlach T, Ideker T, Mali P, Carter H
  TITLE     Interface-guided phenotyping of coding variants in the transcription
            factor RUNX1.
  JOURNAL   Cell Rep. 2024 Jul 4;43(7):114436. doi: 
            10.1016/j.celrep.2024.114436.
  PUBMED    38968069
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-4"; volume: "43(7):114436. doi"; pages: " 
            10.1016/j.celrep.2024.114436"
COMMENT     Sequence Label: EF1A_Hygro_P2A_RUNX1_T65I_Barcode
FEATURES             Location/Qualifiers
     source          1..10865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(5..25)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10..339
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      190..325
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        387..434
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             453..827
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    957..1078
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(994..1013)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1048..1067
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1127..1143)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1127..1143)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1140..1162)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1151..1167
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1175..1181,1182..1199,1200..1205))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1220..1241
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1358..1375)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1529..2117)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1609..1628)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2288..3079,3080..3148))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2911..2930
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3149..3253)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3328..3347)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3519..3898
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3900..4098
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4053..4073
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4116..4296
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4343..4468
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4961..5194
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5379..5423
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5721..5838
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5974..7152
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6205..7143
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7100..7120
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     7169..7188
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7169..7187
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7251..8273
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7251..8273
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8283..8339
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(9893..9926)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             9951..10131
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10157..10174)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10163..10387
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10433..10861
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10520..10539)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10730..10751
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
       61 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
      121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
      181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      241 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
      301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
      361 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
      421 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
      481 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
      541 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
      601 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
      661 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
      721 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
      781 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
      841 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
      901 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
      961 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     1021 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     1081 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     1141 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     1201 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     1261 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     1321 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     1381 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1441 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1501 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1561 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1621 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1681 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1741 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1801 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1861 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1921 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     1981 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2041 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2101 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2161 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2221 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2281 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2341 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     2401 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     2461 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     2521 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     2581 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     2641 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     2701 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     2761 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     2821 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     2881 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     2941 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3001 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3061 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3121 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3181 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3241 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     3301 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     3361 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     3421 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
     3481 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
     3541 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     3601 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     3661 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     3721 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     3781 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     3841 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     3901 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     3961 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     4021 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     4081 ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct
     4141 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     4201 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     4261 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     4321 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     4381 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     4441 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     4501 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     4561 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     4621 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     4681 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     4741 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     4801 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     4861 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     4921 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     4981 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     5041 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     5101 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     5161 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     5221 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     5281 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     5341 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     5401 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     5461 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     5521 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     5581 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     5641 gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa
     5701 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     5761 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     5821 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggttaatt
     5881 aatcgataag ctttgcaaag atggataaag ttttaaacag agaggaatct ttgcagctaa
     5941 tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag
     6001 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
     6061 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     6121 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     6181 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc
     6241 tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt
     6301 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     6361 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga
     6421 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     6481 ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa
     6541 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     6601 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     6661 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     6721 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     6781 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     6841 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     6901 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     6961 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     7021 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     7081 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     7141 caggtgtcgt gaggaattag cttggtacta atacgactca ctatagggag acccaagctg
     7201 gctaggtaag cttgatatcg aattcctgca gcccggggga tcccgccacc atgaaaaagc
     7261 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg
     7321 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
     7381 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
     7441 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca
     7501 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc
     7561 ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg
     7621 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
     7681 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
     7741 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
     7801 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
     7861 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
     7921 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
     7981 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg
     8041 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
     8101 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
     8161 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
     8221 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaaggatccg
     8281 gcgcaacaaa cttctctctg ctgaaacaag ccggagatgt cgaagagaat cctggaccga
     8341 tgcgtatccc cgtagatgcc agcacgagcc gccgcttcac gccgccttcc accgcgctga
     8401 gcccaggcaa gatgagcgag gcgttgccgc tgggcgcccc ggacgccggc gctgccctgg
     8461 ccggcaagct gaggagcggc gaccgcagca tggtggaggt gctggccgac cacccgggcg
     8521 agctggtgcg catcgacagc cccaacttcc tctgctccgt gctgcctacg cactggcgct
     8581 gcaacaagac cctgcccatc gctttcaagg tggtggccct aggggatgtt ccagatggca
     8641 ctctggtcac tgtgatggct ggcaatgatg aaaactactc ggctgagctg agaaatgcta
     8701 ccgcagccat gaagaaccag gttgcaagat ttaatgacct caggtttgtc ggtcgaagtg
     8761 gaagagggaa aagcttcact ctgaccatca ctgtcttcac aaacccaccg caagtcgcca
     8821 cctaccacag agccatcaaa atcacagtgg atgggccccg agaacctcga agacatcggc
     8881 agaaactaga tgatcagacc aagcccggga gcttgtcctt ttccgagcgg ctcagtgaac
     8941 tggagcagct gcggcgcaca gccatgaggg tcagcccaca ccacccagcc cccacgccca
     9001 accctcgtgc ctccctgaac cactccactg cctttaaccc tcagcctcag agtcagatgc
     9061 aggatacaag gcagatccaa ccatccccac cgtggtccta cgatcagtcc taccaatacc
     9121 tgggatccat tgcctctcct tctgtgcacc cagcaacgcc catttcacct ggacgtgcca
     9181 gcggcatgac aaccctctct gcagaacttt ccagtcgact ctcaacggca cccgacctga
     9241 cagcgttcag cgacccgcgc cagttccccg cgctgccctc catctccgac ccccgcatgc
     9301 actatccagg cgccttcacc tactccccga cgccggtcac ctcgggcatc ggcatcggca
     9361 tgtcggccat gggctcggcc acgcgctacc acacctacct gccgccgccc taccccggct
     9421 cgtcgcaagc gcagggaggc ccgttccaag ccagctcgcc ctcctaccac ctgtactacg
     9481 gcgcctcggc cggctcctac cagttctcca tggtgggcgg cgagcgctcg ccgccgcgca
     9541 tcctgccgcc ctgcaccaac gcctccaccg gctccgcgct gctcaacccc agcctcccga
     9601 accagagcga cgtggtggag gccgagggca gccacagcaa ctcccccacc aacatggcgc
     9661 cctccgcgcg cctggaggag gccgtgtgga ggccctactg actagctaag ttagaactat
     9721 ttcctggctg ttacgcgctt cgattttcgc tactgacggg cttgttaaac agtgaaccgc
     9781 ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcgaacaaa
     9841 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tcataacttc
     9901 gtatagcata cattatacga agttatcgcc tagctggtcc ggactgtact gggtctctct
     9961 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
    10021 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
    10081 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt
    10141 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
    10201 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
    10261 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
    10321 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
    10381 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
    10441 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
    10501 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
    10561 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
    10621 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
    10681 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
    10741 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
    10801 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
    10861 taatt
//
