LOCUS       sequence-336118-       10865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of RUNX1 G95R mutant protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, 
            Hutter S, Haferlach T, Ideker T, Mali P, Carter H
  TITLE     Interface-guided phenotyping of coding variants in the transcription
            factor RUNX1.
  JOURNAL   Cell Rep. 2024 Jul 4;43(7):114436. doi: 
            10.1016/j.celrep.2024.114436.
  PUBMED    38968069
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-4"; volume: "43(7):114436. doi"; pages: " 
            10.1016/j.celrep.2024.114436"
COMMENT     Sequence Label: EF1A_Hygro_P2A_RUNX1_G95R_Barcode
FEATURES             Location/Qualifiers
     source          1..10865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     53..74
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(193..213)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        198..527
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      378..513
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        575..622
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             641..1015
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1145..1266
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1182..1201)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1236..1255
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1315..1331)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1315..1331)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1328..1350)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1339..1355
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1363..1369,1370..1387,1388..1393))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1408..1429
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1546..1563)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1717..2305)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1797..1816)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2476..3267,3268..3336))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3099..3118
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3337..3441)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3516..3535)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3707..4086
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4088..4286
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4241..4261
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4304..4484
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4531..4656
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5149..5382
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5567..5611
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5909..6026
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6162..7340
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6393..7331
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7288..7308
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     7357..7376
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7357..7375
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7439..8461
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7439..8461
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8471..8527
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(10081..10114)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             10139..10319
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10345..10362)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10351..10575
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(10621..10865,1..184)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10708..10727)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
       61 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
      121 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
      181 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
      241 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
      301 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
      361 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
      421 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
      481 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
      541 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
      601 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
      661 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
      721 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
      781 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
      841 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
      901 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
      961 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     1021 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     1081 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     1141 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     1201 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     1261 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     1321 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     1381 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     1441 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     1501 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     1561 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1621 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1681 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     1741 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1801 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1861 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     1921 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1981 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     2041 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     2101 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     2161 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2221 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2281 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     2341 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     2401 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     2461 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     2521 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     2581 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     2641 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     2701 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     2761 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     2821 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     2881 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     2941 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     3001 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     3061 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     3121 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     3181 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     3241 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     3301 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     3361 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     3421 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     3481 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
     3541 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     3601 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     3661 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
     3721 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     3781 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     3841 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     3901 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     3961 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     4021 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     4081 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     4141 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     4201 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     4261 gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga
     4321 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     4381 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     4441 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     4501 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     4561 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     4621 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     4681 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     4741 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     4801 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     4861 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     4921 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     4981 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     5041 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     5101 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     5161 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     5221 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     5281 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     5341 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     5401 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     5461 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     5521 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     5581 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     5641 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     5701 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     5761 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     5821 gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct
     5881 gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
     5941 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     6001 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt
     6061 ggttaattaa tcgataagct ttgcaaagat ggataaagtt ttaaacagag aggaatcttt
     6121 gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt
     6181 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     6241 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     6301 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     6361 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     6421 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     6481 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     6541 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     6601 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     6661 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     6721 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     6781 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     6841 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     6901 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     6961 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     7021 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     7081 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     7141 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     7201 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     7261 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     7321 ttccatttca ggtgtcgtga ggaattagct tggtactaat acgactcact atagggagac
     7381 ccaagctggc taggtaagct tgatatcgaa ttcctgcagc ccgggggatc ccgccaccat
     7441 gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
     7501 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
     7561 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
     7621 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
     7681 ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
     7741 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
     7801 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
     7861 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
     7921 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
     7981 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
     8041 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
     8101 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
     8161 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
     8221 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
     8281 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
     8341 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
     8401 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga
     8461 aggatccggc gcaacaaact tctctctgct gaaacaagcc ggagatgtcg aagagaatcc
     8521 tggaccgatg cgtatccccg tagatgccag cacgagccgc cgcttcacgc cgccttccac
     8581 cgcgctgagc ccaggcaaga tgagcgaggc gttgccgctg ggcgccccgg acgccggcgc
     8641 tgccctggcc ggcaagctga ggagcggcga ccgcagcatg gtggaggtgc tggccgacca
     8701 cccgggcgag ctggtgcgca ccgacagccc caacttcctc tgctccgtgc tgcctacgca
     8761 ctggcgctgc aacaagaccc tgcccatcgc tttcaaggtg gtggccctac gggatgttcc
     8821 agatggcact ctggtcactg tgatggctgg caatgatgaa aactactcgg ctgagctgag
     8881 aaatgctacc gcagccatga agaaccaggt tgcaagattt aatgacctca ggtttgtcgg
     8941 tcgaagtgga agagggaaaa gcttcactct gaccatcact gtcttcacaa acccaccgca
     9001 agtcgccacc taccacagag ccatcaaaat cacagtggat gggccccgag aacctcgaag
     9061 acatcggcag aaactagatg atcagaccaa gcccgggagc ttgtcctttt ccgagcggct
     9121 cagtgaactg gagcagctgc ggcgcacagc catgagggtc agcccacacc acccagcccc
     9181 cacgcccaac cctcgtgcct ccctgaacca ctccactgcc tttaaccctc agcctcagag
     9241 tcagatgcag gatacaaggc agatccaacc atccccaccg tggtcctacg atcagtccta
     9301 ccaatacctg ggatccattg cctctccttc tgtgcaccca gcaacgccca tttcacctgg
     9361 acgtgccagc ggcatgacaa ccctctctgc agaactttcc agtcgactct caacggcacc
     9421 cgacctgaca gcgttcagcg acccgcgcca gttccccgcg ctgccctcca tctccgaccc
     9481 ccgcatgcac tatccaggcg ccttcaccta ctccccgacg ccggtcacct cgggcatcgg
     9541 catcggcatg tcggccatgg gctcggccac gcgctaccac acctacctgc cgccgcccta
     9601 ccccggctcg tcgcaagcgc agggaggccc gttccaagcc agctcgccct cctaccacct
     9661 gtactacggc gcctcggccg gctcctacca gttctccatg gtgggcggcg agcgctcgcc
     9721 gccgcgcatc ctgccgccct gcaccaacgc ctccaccggc tccgcgctgc tcaaccccag
     9781 cctcccgaac cagagcgacg tggtggaggc cgagggcagc cacagcaact cccccaccaa
     9841 catggcgccc tccgcgcgcc tggaggaggc cgtgtggagg ccctactgac tagctaagtt
     9901 agaactattt cctggctgtt acgcgttgct tgcgagtcta ctgacgggct tgttaaacag
     9961 tgaaccgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat
    10021 cgaacaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc
    10081 ataacttcgt atagcataca ttatacgaag ttatcgtcta gctggtccgg actgtactgg
    10141 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
    10201 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
    10261 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
    10321 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
    10381 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
    10441 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
    10501 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
    10561 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
    10621 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
    10681 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
    10741 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
    10801 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
    10861 catcg
//
