LOCUS       sequence-336121-       10864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of RUNX1 V97D mutant protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10864)
  AUTHORS   Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, 
            Hutter S, Haferlach T, Ideker T, Mali P, Carter H
  TITLE     Interface-guided phenotyping of coding variants in the transcription
            factor RUNX1.
  JOURNAL   Cell Rep. 2024 Jul 4;43(7):114436. doi: 
            10.1016/j.celrep.2024.114436.
  PUBMED    38968069
REFERENCE   2  (bases 1 to 10864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-4"; volume: "43(7):114436. doi"; pages: " 
            10.1016/j.celrep.2024.114436"
COMMENT     Sequence Label: EF1A_Hygro_P2A_RUNX1_V97D_Barcode
FEATURES             Location/Qualifiers
     source          1..10864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(15..35)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        20..349
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      200..335
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        397..444
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             463..837
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    967..1088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1004..1023)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1058..1077
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1137..1153)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1137..1153)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1150..1172)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1161..1177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1185..1191,1192..1209,1210..1215))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1230..1251
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1368..1385)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1539..2127)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1619..1638)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2298..3089,3090..3158))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2921..2940
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3159..3263)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3338..3357)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3529..3908
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3910..4108
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4063..4083
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4126..4306
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4353..4478
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4971..5204
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5389..5433
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5731..5848
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5984..7162
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6215..7153
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7110..7130
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     7179..7198
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7179..7197
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7261..8283
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7261..8283
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8293..8349
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(9902..9935)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             9960..10140
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10166..10183)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10172..10396
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(10442..10864,1..6)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10529..10548)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10739..10760
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
       61 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
      121 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
      181 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
      241 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
      301 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
      361 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
      421 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
      481 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
      541 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
      601 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
      661 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
      721 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
      781 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
      841 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
      901 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
      961 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     1021 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     1081 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     1141 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     1201 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     1261 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     1321 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     1381 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1441 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1501 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1561 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1621 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1681 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1741 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1801 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1861 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1921 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     1981 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2041 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2101 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2161 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2221 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2281 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2341 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2401 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     2461 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2521 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2581 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     2641 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2701 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2761 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2821 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2881 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     2941 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     3001 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     3061 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     3121 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     3181 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     3241 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     3301 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
     3361 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
     3421 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
     3481 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
     3541 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     3601 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     3661 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     3721 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     3781 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     3841 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     3901 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     3961 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     4021 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     4081 gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta
     4141 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     4201 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     4261 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     4321 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     4381 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     4441 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     4501 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     4561 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     4621 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     4681 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     4741 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     4801 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     4861 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     4921 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     4981 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     5041 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     5101 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     5161 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     5221 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     5281 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     5341 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     5401 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     5461 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     5521 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     5581 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     5641 gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt
     5701 ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     5761 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     5821 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
     5881 ttggttaatt aatcgataag ctttgcaaag atggataaag ttttaaacag agaggaatct
     5941 ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca
     6001 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     6061 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     6121 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     6181 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     6241 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     6301 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     6361 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     6421 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     6481 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     6541 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     6601 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     6661 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     6721 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     6781 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     6841 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     6901 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     6961 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     7021 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     7081 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     7141 tcttccattt caggtgtcgt gaggaattag cttggtacta atacgactca ctatagggag
     7201 acccaagctg gctaggtaag cttgatatcg aattcctgca gcccggggga tcccgccacc
     7261 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     7321 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     7381 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     7441 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     7501 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     7561 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     7621 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     7681 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     7741 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     7801 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     7861 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     7921 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     7981 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     8041 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     8101 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     8161 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     8221 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     8281 gaaggatccg gcgcaacaaa cttctctctg ctgaaacaag ccggagatgt cgaagagaat
     8341 cctggaccga tgcgtatccc cgtagatgcc agcacgagcc gccgcttcac gccgccttcc
     8401 accgcgctga gcccaggcaa gatgagcgag gcgttgccgc tgggcgcccc ggacgccggc
     8461 gctgccctgg ccggcaagct gaggagcggc gaccgcagca tggtggaggt gctggccgac
     8521 cacccgggcg agctggtgcg caccgacagc cccaacttcc tctgctccgt gctgcctacg
     8581 cactggcgct gcaacaagac cctgcccatc gctttcaagg tggtggccct aggggatgat
     8641 ccagatggca ctctggtcac tgtgatggct ggcaatgatg aaaactactc ggctgagctg
     8701 agaaatgcta ccgcagccat gaagaaccag gttgcaagat ttaatgacct caggtttgtc
     8761 ggtcgaagtg gaagagggaa aagcttcact ctgaccatca ctgtcttcac aaacccaccg
     8821 caagtcgcca cctaccacag agccatcaaa atcacagtgg atgggccccg agaacctcga
     8881 agacatcggc agaaactaga tgatcagacc aagcccggga gcttgtcctt ttccgagcgg
     8941 ctcagtgaac tggagcagct gcggcgcaca gccatgaggg tcagcccaca ccacccagcc
     9001 cccacgccca accctcgtgc ctccctgaac cactccactg cctttaaccc tcagcctcag
     9061 agtcagatgc aggatacaag gcagatccaa ccatccccac cgtggtccta cgatcagtcc
     9121 taccaatacc tgggatccat tgcctctcct tctgtgcacc cagcaacgcc catttcacct
     9181 ggacgtgcca gcggcatgac aaccctctct gcagaacttt ccagtcgact ctcaacggca
     9241 cccgacctga cagcgttcag cgacccgcgc cagttccccg cgctgccctc catctccgac
     9301 ccccgcatgc actatccagg cgccttcacc tactccccga cgccggtcac ctcgggcatc
     9361 ggcatcggca tgtcggccat gggctcggcc acgcgctacc acacctacct gccgccgccc
     9421 taccccggct cgtcgcaagc gcagggaggc ccgttccaag ccagctcgcc ctcctaccac
     9481 ctgtactacg gcgcctcggc cggctcctac cagttctcca tggtgggcgg cgagcgctcg
     9541 ccgccgcgca tcctgccgcc ctgcaccaac gcctccaccg gctccgcgct gctcaacccc
     9601 agcctcccga accagagcga cgtggtggag gccgagggca gccacagcaa ctcccccacc
     9661 aacatggcgc cctccgcgcg cctggaggag gccgtgtgga ggccctactg actagctaag
     9721 tagaactatt tcctggctgt tacgcggtac cattatggct actgacgggc ttgttaaaca
     9781 gtgaaccgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca
     9841 tcgaacaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     9901 cataacttcg tatagcatac attatacgaa gttatcgtct agctggtccg gactgtactg
     9961 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
    10021 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
    10081 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
    10141 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
    10201 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
    10261 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
    10321 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
    10381 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
    10441 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
    10501 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
    10561 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
    10621 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
    10681 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
    10741 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
    10801 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
    10861 aacg
//
