LOCUS       sequence-336124-       10865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of RUNX1 T77R mutant protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, 
            Hutter S, Haferlach T, Ideker T, Mali P, Carter H
  TITLE     Interface-guided phenotyping of coding variants in the transcription
            factor RUNX1.
  JOURNAL   Cell Rep. 2024 Jul 4;43(7):114436. doi: 
            10.1016/j.celrep.2024.114436.
  PUBMED    38968069
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-4"; volume: "43(7):114436. doi"; pages: " 
            10.1016/j.celrep.2024.114436"
COMMENT     Sequence Label: EF1A_Hygro_P2A_RUNX1_T77R_Barcode
FEATURES             Location/Qualifiers
     source          1..10865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(85..105)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        90..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        467..514
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             533..907
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1037..1158
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1074..1093)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1128..1147
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1207..1223)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1207..1223)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1220..1242)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1231..1247
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1255..1261,1262..1279,1280..1285))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1300..1321
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1438..1455)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1609..2197)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1689..1708)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2368..3159,3160..3228))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2991..3010
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3229..3333)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3408..3427)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3599..3978
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3980..4178
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4133..4153
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4196..4376
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4423..4548
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5041..5274
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5459..5503
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5801..5918
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6054..7232
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6285..7223
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7180..7200
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     7249..7268
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7249..7267
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7331..8353
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7331..8353
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8363..8419
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(9973..10006)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             10031..10211
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10237..10254)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10243..10467
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(10513..10865,1..76)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10600..10619)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10810..10831
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
       61 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc
      481 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa
      541 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
      601 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
      661 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
      721 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
      781 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
      841 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
      901 ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
      961 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     1021 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     1081 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1141 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     1201 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     1261 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     1321 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     1381 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     1441 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     1501 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     1561 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     1621 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1681 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1741 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1801 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1861 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1921 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1981 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     2041 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     2101 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     2161 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     2221 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     2281 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     2341 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     2401 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     2461 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     2521 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     2581 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     2641 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     2701 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     2761 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     2821 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     2881 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     2941 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     3001 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     3061 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     3121 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     3181 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     3241 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     3301 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     3361 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
     3421 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     3481 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     3541 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
     3601 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     3661 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     3721 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     3781 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     3841 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     3901 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     3961 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     4021 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     4081 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     4141 ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc
     4201 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     4261 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     4321 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     4381 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     4441 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     4501 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     4561 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     4621 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     4681 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     4741 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     4801 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     4861 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     4921 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     4981 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     5041 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     5101 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     5161 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     5221 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     5281 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     5341 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     5401 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     5461 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     5521 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     5581 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     5641 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     5701 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg
     5761 tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     5821 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     5881 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     5941 gagatccagt ttggttaatt aatcgataag ctttgcaaag atggataaag ttttaaacag
     6001 agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg
     6061 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     6121 tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     6181 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     6241 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg
     6301 gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca
     6361 cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt
     6421 cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc
     6481 gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata
     6541 agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata
     6601 gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg
     6661 gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc
     6721 caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg
     6781 cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt
     6841 gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc
     6901 gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag
     6961 ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct
     7021 cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc
     7081 cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct
     7141 tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc
     7201 aaagtttttt tcttccattt caggtgtcgt gaggaattag cttggtacta atacgactca
     7261 ctatagggag acccaagctg gctaggtaag cttgatatcg aattcctgca gcccggggga
     7321 tcccgccacc atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
     7381 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
     7441 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
     7501 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
     7561 gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg
     7621 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga
     7681 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
     7741 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
     7801 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
     7861 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
     7921 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
     7981 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
     8041 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
     8101 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
     8161 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
     8221 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
     8281 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
     8341 gagggcaaag gaaggatccg gcgcaacaaa cttctctctg ctgaaacaag ccggagatgt
     8401 cgaagagaat cctggaccga tgcgtatccc cgtagatgcc agcacgagcc gccgcttcac
     8461 gccgccttcc accgcgctga gcccaggcaa gatgagcgag gcgttgccgc tgggcgcccc
     8521 ggacgccggc gctgccctgg ccggcaagct gaggagcggc gaccgcagca tggtggaggt
     8581 gctggccgac cacccgggcg agctggtgcg caccgacagc cccaacttcc tctgctccgt
     8641 gctgcctagg cactggcgct gcaacaagac cctgcccatc gctttcaagg tggtggccct
     8701 aggggatgtt ccagatggca ctctggtcac tgtgatggct ggcaatgatg aaaactactc
     8761 ggctgagctg agaaatgcta ccgcagccat gaagaaccag gttgcaagat ttaatgacct
     8821 caggtttgtc ggtcgaagtg gaagagggaa aagcttcact ctgaccatca ctgtcttcac
     8881 aaacccaccg caagtcgcca cctaccacag agccatcaaa atcacagtgg atgggccccg
     8941 agaacctcga agacatcggc agaaactaga tgatcagacc aagcccggga gcttgtcctt
     9001 ttccgagcgg ctcagtgaac tggagcagct gcggcgcaca gccatgaggg tcagcccaca
     9061 ccacccagcc cccacgccca accctcgtgc ctccctgaac cactccactg cctttaaccc
     9121 tcagcctcag agtcagatgc aggatacaag gcagatccaa ccatccccac cgtggtccta
     9181 cgatcagtcc taccaatacc tgggatccat tgcctctcct tctgtgcacc cagcaacgcc
     9241 catttcacct ggacgtgcca gcggcatgac aaccctctct gcagaacttt ccagtcgact
     9301 ctcaacggca cccgacctga cagcgttcag cgacccgcgc cagttccccg cgctgccctc
     9361 catctccgac ccccgcatgc actatccagg cgccttcacc tactccccga cgccggtcac
     9421 ctcgggcatc ggcatcggca tgtcggccat gggctcggcc acgcgctacc acacctacct
     9481 gccgccgccc taccccggct cgtcgcaagc gcagggaggc ccgttccaag ccagctcgcc
     9541 ctcctaccac ctgtactacg gcgcctcggc cggctcctac cagttctcca tggtgggcgg
     9601 cgagcgctcg ccgccgcgca tcctgccgcc ctgcaccaac gcctccaccg gctccgcgct
     9661 gctcaacccc agcctcccga accagagcga cgtggtggag gccgagggca gccacagcaa
     9721 ctcccccacc aacatggcgc cctccgcgcg cctggaggag gccgtgtgga ggccctactg
     9781 actagctaag ttagaactat ttcctggctg ttacgcgagt tcgatgcggc tactgacggg
     9841 cttgttaaac agtgaaccgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac
     9901 gaccccatgc atcgaacaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     9961 agacaagata tcataacttc gtatagcata cattatacga agttatcgtc tagctggtcc
    10021 ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
    10081 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
    10141 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
    10201 aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
    10261 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
    10321 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
    10381 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
    10441 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
    10501 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
    10561 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
    10621 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
    10681 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
    10741 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
    10801 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
    10861 ctttt
//
