LOCUS       sequence-336135-       13620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit biosensor to measure realtime
            changes in FRET reflecting fluctuations in cAMP levels in living 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13620)
  TITLE     Jalink Lab Biosensor Plasmids
REFERENCE   2  (bases 1 to 13620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Epac-S-H151
FEATURES             Location/Qualifiers
     source          1..13620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7688..7697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7706..7723
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(7736..7738,7739..7741,7742..8254)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIE"
     CDS             10640..11374
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYIMADKQKNGIKVNFKIRHNIE"
     promoter        11791..11895
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11896..11964,11965..12756)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12114..12133)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12927..13515
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13416..13435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(13603..13620,1)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     13603..13620
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 attgacggcg tagtacacac tattgaatca aacagccgac caattgcact accatcacaa
       61 tggagaagcc agtagtaaac gtagacgtag acccccagag tccgtttgtc gtgcaactgc
      121 aaaaaagctt cccgcaattt gaggtagtag cacagcaggt cactccaaat gaccatgcta
      181 atgccagagc attttcgcat ctggccagta aactaatcga gctggaggtt cctaccacag
      241 cgacgatctt ggacataggc agcgcaccgg ctcgtagaat gttttccgag caccagtatc
      301 attgtgtctg ccccatgcgt agtccagaag acccggaccg catgatgaaa tatgccagta
      361 aactggcgga aaaagcgtgc aagattacaa acaagaactt gcatgagaag attaaggatc
      421 tccggaccgt acttgatacg ccggatgctg aaacaccatc gctctgcttt cacaacgatg
      481 ttacctgcaa catgcgtgcc gaatattccg tcatgcagga cgtgtatatc aacgctcccg
      541 gaactatcta tcatcaggct atgaaaggcg tgcggaccct gtactggatt ggcttcgaca
      601 ccacccagtt catgttctcg gctatggcag gttcgtaccc tgcgtacaac accaactggg
      661 ccgacgagaa agtccttgaa gcgcgtaaca tcggactttg cagcacaaag ctgagtgaag
      721 gtaggacagg aaaattgtcg ataatgagga agaaggagtt gaagcccggg tcgcgggttt
      781 atttctccgt aggatcgaca ctttatccag aacacagagc cagcttgcag agctggcatc
      841 ttccatcggt gttccacttg aatggaaagc agtcgtacac ttgccgctgt gatacagtgg
      901 tgagttgcga aggctacgta gtgaagaaaa tcaccatcag tcccgggatc acgggagaaa
      961 ccgtgggata cgcggttaca cacaatagcg agggcttctt gctatgcaaa gttactgaca
     1021 cagtaaaagg agaacgggta tcgttccctg tgtgcacgta catcccggcc accatatgcg
     1081 atcagatgac tggtataatg gccacggata tatcacctga cgatgcacaa aaacttctgg
     1141 ttgggctcaa ccagcgaatt gtcattaacg gtaggactaa caggaacacc aacaccatgc
     1201 aaaattacct tctgccgatc atagcacaag ggttcagcaa atgggctaag gagcgcaagg
     1261 atgatcttga taacgagaaa atgctgggta ctagagaacg caagcttacg tatggctgct
     1321 tgtgggcgtt tcgcactaag aaagtacatt cgttttatcg cccacctgga acgcagacct
     1381 gcgtaaaagt cccagcctct tttagcgctt ttcccatgtc gtccgtatgg acgacctctt
     1441 tgcccatgtc gctgaggcag aaattgaaac tggcattgca accaaagaag gaggaaaaac
     1501 tgctgcaggt ctcggaggaa ttagtcatgg aggccaaggc tgcttttgag gatgctcagg
     1561 aggaagccag agcggagaag ctccgagaag cacttccacc attagtggca gacaaaggca
     1621 tcgaggcagc cgcagaagtt gtctgcgaag tggaggggct ccaggcggac atcggagcag
     1681 cattagttga aaccccgcgc ggtcacgtaa ggataatacc tcaagcaaat gaccgtatga
     1741 tcggacagta tatcgttgtc tcgccaaact ctgtgctgaa gaatgccaaa ctcgcaccag
     1801 cgcacccgct agcagatcag gttaagatca taacacactc cggaagatca ggaaggtacg
     1861 cggtcgaacc atacgacgct aaagtactga tgccagcagg aggtgccgta ccatggccag
     1921 aattcctagc actgagtgag agcgccacgt tagtgtacaa cgaaagagag tttgtgaacc
     1981 gcaaactata ccacattgcc atgcatggcc ccgccaagaa tacagaagag gagcagtaca
     2041 aggttacaaa ggcagagctt gcagaaacag agtacgtgtt tgacgtggac aagaagcgtt
     2101 gcgttaagaa ggaagaagcc tcaggtctgg tcctctcggg agaactgacc aaccctccct
     2161 atcatgagct agctctggag ggactgaaga cccgacctgc ggtcccgtac aaggtcgaaa
     2221 caataggagt gataggcaca ccggggtcgg gcaagtcagc tattatcaag tcaactgtca
     2281 cggcacgaga tcttgttacc agcggaaaga aagaaaattg tcgcgaaatt gaggccgacg
     2341 tgctaagact gaggggtatg cagattacgt cgaagacagt agattcggtt atgctcaacg
     2401 gatgccacaa agccgtagaa gtgctgtacg ttgacgaagc gttcgcgtgc cacgcaggag
     2461 cactacttgc cttgattgct atcgtcaggc cccgcaagaa ggtagtacta tgcggagacc
     2521 ccatgcaatg cggattcttc aacatgatgc aactaaaggt acatttcaat caccctgaaa
     2581 aagacatatg caccaagaca ttctacaagt atatctcccg gcgttgcaca cagccagtta
     2641 cagctattgt atcgacactg cattacgatg gaaagatgaa aaccacgaac ccgtgcaaga
     2701 agaacattga aatcgatatt acaggggcca caaagccgaa gccaggggat atcatcctga
     2761 catgtttccg cgggtgggtt aagcaattgc aaatcgacta tcccggacat gaagtaatga
     2821 cagccgcggc ctcacaaggg ctaaccagaa aaggagtgta tgccgtccgg caaaaagtca
     2881 atgaaaaccc actgtacgcg atcacatcag agcatgtgaa cgtgttgctc acccgcactg
     2941 aggacaggct agtgtggaaa accttgcagg gcgacccatg gattaagcag ctcactaaca
     3001 tacctaaagg aaactttcag gctactatag aggactggga agctgaacac aagggaataa
     3061 ttgctgcaat aaacagcccc actccccgtg ccaatccgtt cagctgcaag accaacgttt
     3121 gctgggcgaa agcattggaa ccgatactag ccacggccgg tatcgtactt accggttgcc
     3181 agtggagcga actgttccca cagtttgcgg atgacaaacc acattcggcc atttacgcct
     3241 tagacgtaat ttgcattaag tttttcggca tggacttgac aagcggactg ttttctaaac
     3301 agagcatccc actaacgtac catcccgccg attcagcgag gccggtagct cattgggaca
     3361 acagcccagg aacccgcaag tatgggtacg atcacgccat tgccgccgaa ctctcccgta
     3421 gatttccggt gttccagcta gctgggaagg gcacacaact tgatttgcag acggggagaa
     3481 ccagagttat ctctgcacag cataacctgg tcccggtgaa ccgcaatctt cctcacgcct
     3541 tagtccccga gtacaaggag aagcaacccg gcccggtcga aaaattcttg aaccagttca
     3601 aacaccactc agtacttgtg gtatcagagg aaaaaattga agctccccgt aagagaatcg
     3661 aatggatcgc cccgattggc atagccggtg cagataagaa ctacaacctg gctttcgggt
     3721 ttccgccgca ggcacggtac gacctggtgt tcatcaacat tggaactaaa tacagaaacc
     3781 accactttca gcagtgcgaa gaccatgcgg cgaccttaaa aaccctttcg cgttcggccc
     3841 tgaattgcct taacccagga ggcaccctcg tggtgaagtc ctatggctac gccgaccgca
     3901 acagtgagga cgtagtcacc gctcttgcca gaaagtttgt cagggtgtct gcagcgagac
     3961 cagattgtgt ctcaagcaat acagaaatgt acctgatttt ccgacaacta gacaacagcc
     4021 gtacacggca attcaccccg caccatctga attgcgtgat ttcgtccgtg tatgagggta
     4081 caagagatgg agttggagcc gcgccgtcat accgcaccaa aagggagaat attgctgact
     4141 gtcaagagga agcagttgtc aacgcagcca atccgctggg tagaccaggc gaaggagtct
     4201 gccgtgccat ctataaacgt tggccgacca gttttaccga ttcagccacg gagacaggca
     4261 ccgcaagaat gactgtgtgc ctaggaaaga aagtgatcca cgcggtcggc cctgatttcc
     4321 ggaagcaccc agaagcagaa gccttgaaat tgctacaaaa cgcctaccat gcagtggcag
     4381 acttagtaaa tgaacataac atcaagtctg tcgccattcc actgctatct acaggcattt
     4441 acgcagccgg aaaagaccgc cttgaagtat cacttaactg cttgacaacc gcgctagaca
     4501 gaactgacgc ggacgtaacc atctattgcc tggataagaa gtggaaggaa agaatcgacg
     4561 cggcactcca acttaaggag tctgtaacag agctgaagga tgaagatatg gagatcgacg
     4621 atgagttagt atggattcat ccagacagtt gcttgaaggg aagaaaggga ttcagtacta
     4681 caaaaggaaa attgtattcg tacttcgaag gcaccaaatt ccatcaagca gcaaaagaca
     4741 tggcggagat aaaggtcctg ttccctaatg accaggaaag taatgaacaa ctgtgtgcct
     4801 acatattggg tgagaccatg gaagcaatcc gcgaaaagtg cccggtcgac cataacccgt
     4861 cgtctagccc gcccaaaacg ttgccgtgcc tttgcatgta tgccatgacg ccagaaaggg
     4921 tccacagact tagaagcaat aacgtcaaag aagttacagt atgctcctcc accccccttc
     4981 ctaagcacaa aattaagaat gttcagaagg ttcagtgcac gaaagtagtc ctgtttaatc
     5041 cgcacactcc cgcattcgtt cccgcccgta agtacataga agtgccagaa cagcctaccg
     5101 ctcctcctgc acaggccgag gaggcccccg aagttgtagc gacaccgtca ccatctacag
     5161 ctgataacac ctcgcttgat gtcacagaca tctcactgga tatggatgac agtagcgaag
     5221 gctcactttt ttcgagcttt agcggatcgg acaactctat tactagtatg gacagttggt
     5281 cgtcaggacc tagttcacta gagatagtag accgaaggca ggtggtggtg gctgacgttc
     5341 atgccgtcca agagcctgcc cctattccac cgccaaggct aaagaagatg gcccgcctgg
     5401 cagcggcaag aaaagagccc actccaccgg caagcaatag ctctgagtcc ctccacctct
     5461 cttttggtgg ggtatccatg tccctcggat caattttcga cggagagacg gcccgccagg
     5521 cagcggtaca acccctggca acaggcccca cggatgtgcc tatgtctttc ggatcgtttt
     5581 ccgacggaga gattgatgag ctgagccgca gagtaactga gtccgaaccc gtcctgtttg
     5641 gatcatttga accgggcgaa gtgaactcaa ttatatcgtc ccgatcagcc gtatcttttc
     5701 ctctacgcaa gcagagacgt agacgcagga gcaggaggac tgaatactga ctaaccgggg
     5761 taggtgggta catattttcg acggacacag gccctgggca cttgcaaaag aagtccgttc
     5821 tgcagaacca gcttacagaa ccgaccttgg agcgcaatgt cctggaaaga attcatgccc
     5881 cggtgctcga cacgtcgaaa gaggaacaac tcaaactcag gtaccagatg atgcccaccg
     5941 aagccaacaa aagtaggtac cagtctcgta aagtagaaaa tcagaaagcc ataaccactg
     6001 agcgactact gtcaggacta cgactgtata actctgccac agatcagcca gaatgctata
     6061 agatcaccta tccgaaacca ttgtactcca gtagcgtacc ggcgaactac tccgatccac
     6121 agttcgctgt agctgtctgt aacaactatc tgcatgagaa ctatccgaca gtagcatctt
     6181 atcagattac tgacgagtac gatgcttact tggatatggt agacgggaca gtcgcctgcc
     6241 tggatactgc aaccttctgc cccgctaagc ttagaagtta cccgaaaaaa catgagtata
     6301 gagccccgaa tatccgcagt gcggttccat cagcgatgca gaacacgcta caaaatgtgc
     6361 tcattgccgc aactaaaaga aattgcaacg tcacgcagat gcgtgaactg ccaacactgg
     6421 actcagcgac attcaatgtc gaatgctttc gaaaatatgc atgtaatgac gagtattggg
     6481 aggagttcgc tcggaagcca attaggatta ccactgagtt tgtcaccgca tatgtagcta
     6541 gactgaaagg ccctaaggcc gccgcactat ttgcaaagac gtataatttg gtcccattgc
     6601 aagaagtgcc tatggataga ttcgtcatgg acatgaaaag agacgtgaaa gttacaccag
     6661 gcacgaaaca cacagaagaa agaccgaaag tacaagtgat acaagccgca gaacccctgg
     6721 cgactgctta cttatgcggg attcaccggg aattagtgcg taggcttacg gccgtcttgc
     6781 ttccaaacat tcacacgctt tttgacatgt cggcggagga ttttgatgca atcatagcag
     6841 aacacttcaa gcaaggcgac ccggtactgg agacggatat cgcatcattc gacaaaagcc
     6901 aagacgacgc tatggcgtta accggtctga tgatcttgga ggacctgggt gtggatcaac
     6961 cactactcga cttgatcgag tgcgcctttg gagaaatatc atccacccat ctacctacgg
     7021 gtactcgttt taaattcggg gcgatgatga aatccggaat gttcctcaca ctttttgtca
     7081 acacagtttt gaatgtcgtt atcgccagca gagtactaga agagcggctt aaaacgtcca
     7141 gatgtgcagc gttcattggc gacgacaaca tcatacatgg agtagtatct gacaaagaaa
     7201 tggctgagag gtgcgccacc tggctcaaca tggaggttaa gatcatcgac gcagtcatcg
     7261 gtgagagacc accttacttc tgcggcggat ttatcttgca agattcggtt acttccacag
     7321 cgtgccgcgt ggcggatccc ctgaaaaggc tgtttaagtt gggtaaaccg ctcccagccg
     7381 acgacgagca agacgaagac agaagacgcg ctctgctaga tgaaacaaag gcgtggttta
     7441 gagtaggtat aacaggcact ttagcagtgg ccgtgacgac ccggtatgag gtagacaata
     7501 ttacacctgt cctactggca ttgagaactt ttgcccagag caaaagagca ttccaagcca
     7561 tcagagggga aataaagcat ctctacggtg gtcctaaata gtcagcatag tacatttcat
     7621 ctgactaata ccacaacacc accacctcta gacgcgtaga tctcacgtga gcatgcagga
     7681 gcttgccgcc accatggggg gttctcatca tcatcatcat catggtgcgg ccgcaatggt
     7741 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     7801 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     7861 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     7921 gaccaccctg tcctggggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
     7981 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     8041 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     8101 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     8161 ggagtacaac tactttagcg acaacgtcta tatcaccgcc gacaagcaga agaacggcat
     8221 caaggccaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca
     8281 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     8341 gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     8401 ggagttcgtg accgccgccc gatatcagcc cgtgggaact catgagatgg aggaggagtt
     8461 ggccgaagct gtggccctgc tctcccagcg ggggcctgac gccctgctca ctgtggcact
     8521 tcgaaagccc ccaggtcagc gcacggatga agagctggac ctcatctttg aggagctgct
     8581 gcacatcaag gctgtggccc acctctccaa ctcggtgaag cgagaattag cggctgttct
     8641 gctctttgaa ccacacagca aggcagggac cgtgttgttc agccaggggg acaagggcac
     8701 ttcgtggtac attatctgga agggatctgt caacgtggtg acccatggca aggggctggt
     8761 gaccaccctg catgagggag atgattttgg acagctggct ctggtgaatg atgcaccccg
     8821 ggcagccacc atcatcctgc gagaagacaa ctgtcatttc ctgcgtgtgg acaagcagga
     8881 cttcaaccgt atcatcaagg atgtggaggc aaagaccatg cggctggaag aacatggcaa
     8941 agtggtgctg gtgctggaga gagcctctca gggcgccggc ccttcccgac ccccaacccc
     9001 aggcaggaac cggtatacag tgatgtctgg caccccagag aagatcctag agcttctgtt
     9061 ggaggccatg ggaccagatt ccagtgctca tgacccaaca gagacattcc tcagcgactt
     9121 cctcctgacc cacagggtct tcatgcccag cgcccaactc tgcgctgccc ttctgcacca
     9181 cttccatgtg gagcctgcgg gtggcagcga gcaggagcgc agcacctacg tctgcaacaa
     9241 gaggcagcag atcttgcggc tggtcagcca gtgggtggcc ctgtatggct ccatgctcca
     9301 cactgaccct gtggccacca gcttcctcca gaaactctca gacctggtgg gcagggacac
     9361 ccgactcagc aacctgctga gggagcagtg gccagagagg cggcgatgcc acaggttgga
     9421 gaatggctgt gggaatgcat ctcctcagat gaaggcccgg aacttgcctg tttggctccc
     9481 caaccaggac gagccccttc ctggcagcag ctgtgccatc caagttgggg ataaagtccc
     9541 ctatgacatc tgccggccag accactcagt gttgaccctg cagctgcctg tgacagcctc
     9601 cgtgagagag gtgatggcag cgttggccca ggaggatggc tggaccaagg ggcaggtgct
     9661 ggtgaaggtc aattctgcag gtgatgccat tggcctgcag ccagatgccc gtggtgtggc
     9721 cacatctctg gggctcaatg agcgtctctt tgttgtcaac ccacaggaag cgcatgagct
     9781 gatcccacac cctgaccagc tggggcccac tgtgggctct gctgaggggc tggacctggt
     9841 gagtgccaag gacctggcag gccagctgac ggaccacgac tggagcctct tcaacagtat
     9901 ccaccaggtg gagctgatcc actatgtgct gggcccccag catctgcggg atgtcaccac
     9961 cgccaacctg gagcgcttca tgcgccgctt caatgagctg cagtactggg tggccaccga
    10021 gctgtgtctc tgccccgtgc ccggcccccg ggcccagctg ctcaggaagt tcattaagct
    10081 ggcggcccac ctcaaggagc agaagaatct caattccttc tttgccgtca tgtttggcct
    10141 cagcaactcg gccatcagcc gcctagccca cacctgggag cggctgcccc acaaagtccg
    10201 gaagctgtac tccgccctcg agaggctgct ggatccctca tggaaccacc gggtataccg
    10261 actggccctc gccaagctct cccctcctgt catccccttc atgccccttc ttctcaaaga
    10321 catggccgcc attcatgagg gaaaccacac actagtggag aatctcatca actttgagaa
    10381 gatgagaatg atggccagag ccgcgcggat gctgcaccac tgccgaagcc acaaccctgt
    10441 gcctctctca ccactcagaa gccgagtttc ccacctccac gaggacagcc aggtggcgag
    10501 gatttccaca tgctcggagc agtccctgag cacccggagt ccagccagca cctgggctta
    10561 tgtccagcag ctgaaggtca ttgacaacca gcgggaactc tcccgcctct cccgagagct
    10621 ggagccagct agcagctcaa tgggcagcgt gcagctcgcc gaccactacc agcagaacac
    10681 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc
    10741 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
    10801 cgccgggatc actctcggca tggacgagct gtacaagggc ggtaccggcg gcagcatggt
    10861 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
    10921 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
    10981 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
    11041 gaccaccttc ggctacggcc tgatgtgctt cgcccgctac cccgaccaca tgaagcagca
    11101 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
    11161 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
    11221 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
    11281 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
    11341 caaggtgaac ttcaagatcc gccacaacat cgagccgaat tccctcgagg ttccttgggc
    11401 ccaatgatcc gaccagcaaa actcgatgta cttccgagga actgatgtgc ataatgcatc
    11461 aggctggtac attagatccc cgcttaccgc gggcaatata gcaacactaa aaactcgatg
    11521 tacttccgag gaagcgcagt gcataatgct gcgcagtgtt gccacataac cactatatta
    11581 accatttatc tagcggacgc caaaaactca atgtatttct gaggaagcgt ggtgcataat
    11641 gccacgcagc gtctgcataa cttttattat ttcttttatt aatcaacaaa atttgataat
    11701 taagcggccg ctcgagcggc cgcttaatta atcgagggga attaattctt gaagacgaaa
    11761 gggccaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
    11821 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
    11881 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
    11941 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
    12001 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
    12061 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
    12121 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
    12181 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
    12241 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
    12301 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
    12361 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
    12421 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
    12481 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
    12541 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
    12601 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
    12661 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
    12721 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
    12781 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
    12841 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
    12901 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
    12961 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
    13021 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
    13081 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
    13141 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
    13201 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
    13261 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
    13321 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    13381 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    13441 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    13501 cggagcctat ggaaaaacgc cagcaacgcg agctcgtatg gacatattgt cgttagaacg
    13561 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactatag
//
