LOCUS       sequence-336139-        9279 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide
            targeting exon 13. Expressing nuclear RFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9279)
  AUTHORS   Jonsson ME, Garza R, Sharma Y, Petri R, Sodersten E, Johansson JG, 
            Johansson PA, Atacho DA, Pircs K, Madsen S, Yudovich D, Ramakrishnan
            R, Holmberg J, Larsson J, Jern P, Jakobsson J
  TITLE     Activation of endogenous retroviruses during brain development 
            causes an inflammatory response.
  JOURNAL   EMBO J. 2021 Mar 1:e106423. doi: 10.15252/embj.2020106423.
  PUBMED    33644903
REFERENCE   2  (bases 1 to 9279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2021 Mar 1:e106423. doi: 10.15252/embj.2020106423."
COMMENT     Sequence Label: pLV.U6gT28.13.EFS-NS.H2B-RFP
FEATURES             Location/Qualifiers
     source          1..9279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(14..33)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        205..584
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        586..784
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     739..759
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             802..982
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1029..1154
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1647..1880
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2065..2109
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2407..2524
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2575..2823
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2575..2595
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2746..2765
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2845..2920
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2982..3193
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             3213..3590
                     /codon_start=1
                     /gene="HIST1H2BB"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPSKSAPAPKKGSKKAITKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSSK"
     regulatory      3603..3612
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3609..4319
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3754..3772)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4022..4041
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4338..4926
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4391..4411)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4809..4820)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4998..5231
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5257..5274)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5263..5487
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5533..5961
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5620..5639)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5830..5851
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5970..5990)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5975..6304
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6155..6290
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6217..6236
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6352..6399
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6418..6792
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6922..7043
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6959..6978)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7013..7032
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7092..7108)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7092..7108)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7105..7127)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7116..7132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7140..7146,7147..7164,7165..7170))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7185..7206
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7323..7340)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7494..8082)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7574..7593)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8253..9044,9045..9113))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8876..8895
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9114..9218)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
       61 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
      121 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
      181 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
      241 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      301 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      361 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      421 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      481 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      541 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      601 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      661 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      721 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      781 gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
      841 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
      901 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
      961 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     1021 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     1081 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     1141 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     1201 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     1261 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     1321 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     1381 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     1441 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     1501 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     1561 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     1621 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     1681 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     1741 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     1801 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     1861 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     1921 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     1981 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     2041 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     2101 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     2161 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     2221 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     2281 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     2341 cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat
     2401 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     2461 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     2521 ttttcgggtt tattacaggg acagcagaga tccagtttgg ttaattaagg taccgagggc
     2581 ctatttccca tgattccttc atatttgcat atacgataca aggctgttag agagataatt
     2641 agaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa
     2701 taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt
     2761 accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac
     2821 accgcgcact ggttacacat gaccgtttta gagctagaaa tagcaagtta aaataaggct
     2881 agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttgaat tcgctagcta
     2941 ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc
     3001 cacagtcccc gagaagttgg ggggaggggt cggcaattga tccggtgcct agagaaggtg
     3061 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     3121 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     3181 cgccagaaca caggaccggt tctagagcca ccatgcctga accctctaag tctgctccag
     3241 cccctaaaaa gggttctaag aaggctatca ctaaggcgca gaagaaggat ggtaagaagc
     3301 gtaagcgcag ccgcaaggag agctattcta tctatgtgta caaggttctg aagcaggtcc
     3361 accccgacac cggcatctca tccaaggcca tggggatcat gaactccttc gtcaacgaca
     3421 tcttcgagcg catcgcgggc gaggcttctc gcctggctca ctacaataag cgctcgacca
     3481 tcacctccag ggagattcag acggctgtgc gcctgctgct gcctggggag ctggctaagc
     3541 atgctgtgtc cgagggcact aaggcagtta ccaagtacac tagctctaag gatccaccgg
     3601 tcgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
     3661 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     3721 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     3781 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     3841 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     3901 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     3961 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     4021 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     4081 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     4141 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     4201 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     4261 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaaa
     4321 ctagtggatc cgtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     4381 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     4441 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     4501 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     4561 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     4621 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     4681 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     4741 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     4801 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     4861 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     4921 ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta gccacttttt
     4981 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     5041 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     5101 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     5161 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     5221 aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     5281 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     5341 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     5401 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     5461 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     5521 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     5581 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     5641 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     5701 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     5761 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     5821 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     5881 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     5941 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     6001 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     6061 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     6121 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     6181 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     6241 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     6301 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
     6361 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
     6421 gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag
     6481 ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg
     6541 gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac
     6601 accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc
     6661 gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg
     6721 tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag
     6781 gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
     6841 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     6901 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     6961 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     7021 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     7081 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     7141 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     7201 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     7261 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     7321 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     7381 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7441 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7501 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7561 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7621 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7681 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7741 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7801 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7861 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7921 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7981 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8041 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8101 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8161 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8221 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8281 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     8341 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     8401 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8461 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8521 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8581 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8641 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     8701 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8761 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8821 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8881 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8941 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9001 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9061 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9121 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9181 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9241 cctgacgtcg acggatcggg agatctcccg atcccctat
//
