LOCUS       sequence-336152-        6118 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses jGCaMP7c variant 1513 in astrocytes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Chneiweiss, H
  TITLE     Chneiweiss Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-GFAP-jGCaMP7c variant 1513
FEATURES             Location/Qualifiers
     source          1..6118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     regulatory      867..876
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(873..881,882..899,900..902,903..935,936..938,939..962,
                     963..989,990..1046,1047..1049,1050..1052,1053..1322,
                     1323..1340,1341..1775,1776..1781,1782..2225)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6m
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSQPNVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKGSYRDSEEEIREAFGVFDKDGN
                     GYISAAELRHVMNNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      969..978
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1259..1280
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1386..1407)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1647..1666)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2245..2833
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2298..2318)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2716..2727)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2865..3341
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3064..3083)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3381..3521
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3381..3510
                     /label=AAV2 ITR
     rep_origin      3596..4051
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3683..3702)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3893..3914
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4068..4087)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4187..4209
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4247..4265)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4333..4437
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4438..4506,4507..5298)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5469..6057
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5958..5977
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagagatcta acatatcctg
      181 gtgtggagta ggggacgctg ctctgacaga ggctcggggg cctgagctgg ctctgtgagc
      241 tggggaggag gcagacagcc aggccttgtc tgcaagcaga cctggcagca ttgggctggc
      301 cgccccccag ggcctcctct tcatgcccag tgaatgactc accttggcac agacacaatg
      361 ttcggggtgg gcacagtgcc tgcttcccgc cgcaccccag cccccctcaa atgccttccg
      421 agaagcccat tgagcagggg gcttgcattg caccccagcc tgacagcctg gcatcttggg
      481 ataaaagcag cacagccccc taggggctgc ccttgctgtg tggcgccacc ggcggtggag
      541 aacaaggctc tattcagcct gtgcccagga aaggggatca ggggatgccc aggcatggac
      601 agtgggtggc agggggggag aggagggctg tctgcttccc agaagtccaa ggacacaaat
      661 gggtgagggg agagctctcc ccatagctgg gctgcggccc aaccccaccc cctcaggcta
      721 tgccaggggg tgttgccagg ggcacccggg catcgccagt ctagcccact ccttcataaa
      781 gccctcgcat cccaggagcg agcagagcca gagcaggttg gagaggagac gcatcacctc
      841 cgctgctcgc ggggtctaga gtcgacgcca ccatgggttc tcatcatcat catcatcatg
      901 gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac gatgacgata
      961 aggatctcgc caccatggtc gactcatcac gtcgtaagtg gaataagaca ggtcacgcag
     1021 tcagagctat aggtcggctg agctcacagc ctaacgtcta tatcaaggcc gacaagcaga
     1081 agaacggcat caaggcgaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc
     1141 tcgcctacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     1201 accactacct gagcgtgcag tccaaacttt cgaaagaccc caacgagaag cgcgatcaca
     1261 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     1321 agggcggtac cggagggagc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     1381 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     1441 gtgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     1501 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     1561 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctaca
     1621 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     1681 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     1741 acggcaacat cctggggcac aagctggagt acaaccttcc tgaccaactg actgaagagc
     1801 agatcgcaga atttaaagag gctttctccc tatttgacaa ggacggggat gggacaataa
     1861 caaccaagga gctggggacg gtgatgcgtt ctctggggca gaaccccaca gaagcagagc
     1921 tgcaggacat gatcaatgaa gtagatgccg acggtgacgg cacaatcgac ttccctgagt
     1981 tcctgacaat gatggcaaga aaagggagct acagggacag tgaagaagaa attagagaag
     2041 cgttcggtgt gtttgataag gatggcaatg gctacatcag tgcagcagag cttcgccacg
     2101 tgatgaacaa ccttggagag aagttaacag atgaagaggt tgatgaaatg atcagggaag
     2161 cagacatcga tggggatggt caggtaaact acgaagagtt tgtacaaatg atgacagcga
     2221 agtgacgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     2281 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     2341 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     2401 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     2461 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     2521 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     2581 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     2641 tcatcgtcct ttccttggct gctcgcctat gttgccacct ggattctgcg cgggacgtcc
     2701 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     2761 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     2821 gccgcctccc cgcatcgata ccgagcgctg ctcgagagat ctacgggtgg catccctgtg
     2881 acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg
     2941 tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg
     3001 ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt agggcctgcg
     3061 gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg
     3121 cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat
     3181 gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg
     3241 ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc
     3301 tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgattttg taggtaacca
     3361 cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
     3421 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     3481 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt
     3541 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
     3601 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     3661 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     3721 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     3781 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     3841 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     3901 ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg
     3961 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     4021 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
     4081 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     4141 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     4201 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     4261 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     4321 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     4381 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     4441 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     4501 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     4561 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     4621 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     4681 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     4741 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     4801 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     4861 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     4921 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     4981 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     5041 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     5101 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     5161 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     5221 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     5281 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     5341 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5401 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5461 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5521 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5581 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     5641 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     5701 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     5761 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     5821 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     5881 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     5941 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6001 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6061 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
