LOCUS       sequence-33616-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, 
            Eichtinger M, Jiang T, Foley JE, Winfrey RJ, Townsend JA, 
            Unger-Wallace E, Sander JD, Muller-Lerch F, Fu F, Pearlberg J, 
            Goebel C, Dassie JP, Pruett-Miller SM, Porteus MH, Sgroi DC, Iafrate
            AJ, Dobbs D, McCray PB, Cathomen T, Voytas DF, Joung JK
  TITLE     Rapid ""open-source"" engineering of customized zinc-finger 
            nucleases for highly efficient gene modification.
  JOURNAL   Mol Cell. 2008 Jul 25. 31(2):294-301.
  PUBMED    18657511
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2008 Jul 25. 31(2):294-301."
COMMENT     Sequence Label: cftr_R (OZ518)
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..44
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     rep_origin      446..873
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(533..552)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     743..764
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tccaagcttt ggcggccgct gactacaaag acgatgatga caaatctaga cccggggagc
       61 gccccttcca gtgtcgcatt tgcatgcgga acttttcgac ccacgcccac cttaccaggc
      121 atacccgtac tcataccggt gaaaaaccgt ttcagtgtcg gatctgtatg cgaaatttct
      181 ccgacggcag caacttgcgc cgccatctac gtacgcacac cggcgagaag ccattccaat
      241 gccgaatatg catgcgcaac ttcagtcgga tcgacaacct ggacgggcac ctaaaaaccc
      301 acctgagggg atcctctgca cagtagtaac tagagactag aaaaaggccg acaagtcccg
      361 ctccgctgaa gatcctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
      421 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
      481 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
      541 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
      601 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
      661 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
      721 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
      781 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
      841 atgagctgat ttaacaaaaa tttaacgcga attttaacaa antcttagcg ctgaggt
//
