LOCUS       sequence-336167-        9789 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cell-free/mammalian expression vector for SARS-COV-2 virus S with 
            N-term eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9789)
  TITLE     Alexandrov Lab Plasmids
REFERENCE   2  (bases 1 to 9789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pmCellFree_KA1_SARS-COV-2_S
FEATURES             Location/Qualifiers
     source          1..9789
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     181..1008
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             181..305
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(343..948)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     enhancer        1140..1443
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1449..1726
                     /label=chicken beta-actin promoter
     primer_bind     2005..2027
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2089..2108
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     2147..2166
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2147..2165
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2309..2332
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             2339..3049
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     CDS             2339..3049
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(2639..2658)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2986..3007
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3050..3073
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    3074..3098
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6918..6942)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6977..6994
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7084..7103)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7149..7204
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7203..7222)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     terminator      7292..7339
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_recomb     7352..8057
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           7440..7812
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(7469..8057)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     protein_bind    complement(8066..8099)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(8267..8854)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8346..8365)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8867..9658,9659..9727))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9490..9509
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccg
     1021 taatgagacg cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctct
     1081 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     1141 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     1201 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     1261 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     1321 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     1381 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     1441 atgcatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
     1501 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
     1561 gggggggggg cgcgcgccgg gggggggggg ggggggsggg gggsggggsg gggsgaggcg
     1621 gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
     1681 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
     1741 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
     1801 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctccttcgg gctgtaatta
     1861 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
     1921 ccgggagggc cctttgtgcg gggggagcgg ctcggggctg tccgcggggg gacggctgcc
     1981 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     2041 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     2101 ttattgtgct gtctcatcat tttggcaaag aattggatcg gacgtctaat acgactcact
     2161 atagggacat cttaagttta ttttatttta ttttatttta ttttatttta ttttatttta
     2221 ttttatttta ttttatttaa ccatgacagt aatgtataaa gtctgtaaag acattaaaca
     2281 cgtaagtgaa accatgggca cctctggtca ccaccatcac caccatcacc acggcacaag
     2341 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagttgg acggcgacgt
     2401 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     2461 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     2521 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     2581 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     2641 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     2701 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     2761 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     2821 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     2881 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     2941 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     3001 gttcgtgacc gccgccggga tcactctcgg catggacgag ctatacaagc tggaggtgct
     3061 cttccagggt ccgacaagtt tgtacaaaaa agttggcatg ttcgtgttcc tggtgctgct
     3121 gccactggtg agctcccagt gcgtgaacct gaccaccaga acccagctgc cacctgccta
     3181 caccaactcc ttcaccaggg gcgtgtacta cccagacaag gtgttcagat ccagcgtgct
     3241 gcactctacc caggacctgt tcctgccatt cttctctaac gtgacctggt tccacgccat
     3301 ccacgtgtct ggaaccaacg gcaccaagag attcgacaac ccagtgctgc cattcaacga
     3361 cggcgtgtac ttcgcttcta ccgagaagtc taacatcatc aggggttgga tcttcggaac
     3421 caccctggac tctaagaccc agtctctgct gatcgtgaac aacgctacca acgtggtgat
     3481 caaggtgtgc gagttccagt tctgcaacga cccattcctg ggagtgtact accacaagaa
     3541 caacaagtct tggatggaaa gcgagttcag ggtgtacagc tctgctaaca actgcacctt
     3601 cgagtacgtg tctcagcctt tcctgatgga cctggaagga aagcagggaa acttcaagaa
     3661 cctgagagag ttcgtgttca agaacatcga cggttacttc aagatataca gcaagcacac
     3721 cccaatcaac ctggtgaggg acctgccaca gggtttctct gctctggagc ccctggtgga
     3781 cctgccaatc ggcatcaaca tcaccaggtt ccagaccctg ctggctctgc acaggtctta
     3841 cctgacccca ggcgacagca gctctggatg gaccgccggc gccgctgctt actacgtggg
     3901 atacctgcag ccaaggacct tcctgctgaa gtacaacgaa aacggcacca tcaccgacgc
     3961 cgtggactgc gctctggacc cactgtctga gaccaagtgc accctgaagt ctttcaccgt
     4021 ggaaaaggga atctaccaga ccagcaactt ccgcgtgcag cccaccgagt ctatcgtgag
     4081 attcccaaac atcaccaacc tgtgcccatt cggagaagtg ttcaacgcta ccaggttcgc
     4141 cagcgtgtac gcttggaaca ggaagagaat cagcaactgc gtggccgact actctgtgct
     4201 gtacaactct gcctccttct ccaccttcaa gtgctacggc gtgtccccaa ccaagctgaa
     4261 cgacctgtgc ttcaccaacg tgtacgctga ctccttcgtg atcagaggag acgaagtgag
     4321 acagatcgct cccggtcaga ccggaaagat cgccgactac aactacaagc tgccagacga
     4381 cttcaccggt tgcgtgatcg cttggaactc taacaacctg gactctaagg tgggaggcaa
     4441 ctacaactac ctgtacaggc tgttccgcaa gagcaacctg aagcctttcg aaagggacat
     4501 cagcaccgaa atctaccagg ctggatctac cccatgcaac ggagtggaag gattcaactg
     4561 ctacttccct ctgcagtctt acggattcca gccaaccaac ggagtgggat accagccata
     4621 cagagtggtg gtgctgtctt tcgagctgct gcacgctcct gctaccgtgt gcggaccaaa
     4681 gaagtctacc aacctggtga agaacaagtg cgtgaacttc aacttcaacg gactgaccgg
     4741 aaccggagtg ctgaccgaat ccaacaagaa gttcctgcct ttccagcagt tcggtagaga
     4801 catcgccgac accaccgacg ctgtgagaga cccacagacc ctggaaatcc tggacatcac
     4861 cccctgctct ttcggaggag tgagcgtgat caccccagga accaacacct ccaaccaggt
     4921 ggccgtgctg taccaggacg tgaactgcac cgaagtgcca gtggccatcc acgctgacca
     4981 gctgacccca acctggagag tgtacagcac cggatctaac gtgttccaga cccgtgccgg
     5041 ctgcctgatc ggagccgagc acgtgaacaa ctcctacgaa tgcgacatcc caatcggagc
     5101 tggcatctgc gcttcttacc agacccagac caacagccca agaagggcca gatccgtggc
     5161 ctctcagagc atcatcgcct acaccatgag cctgggcgct gaaaacagcg tggcttacag
     5221 caacaactct atcgccatcc caaccaactt caccatctct gtgaccaccg aaatcctgcc
     5281 agtgagcatg accaagacca gcgtggactg caccatgtac atctgcggag actctaccga
     5341 atgctctaac ctgctgctgc agtacggcag cttctgcacc cagctgaacc gcgctctgac
     5401 cggaatcgct gtggagcagg acaagaacac ccaggaagtg ttcgctcagg tgaagcagat
     5461 atacaagacc ccacctatca aggacttcgg aggattcaac ttctctcaga tcctgcccga
     5521 cccatctaag ccctctaaga ggtctttcat cgaagacctg ctgttcaaca aggtgaccct
     5581 ggccgacgcc ggtttcatca agcagtacgg tgactgcctg ggagacatcg ccgctagaga
     5641 cctgatctgc gcccagaagt tcaacggtct gaccgtgctg ccaccactgc tgaccgacga
     5701 gatgatcgcc cagtacacct ctgccctgct ggctggaacc atcacctctg gttggacctt
     5761 cggagctggc gccgccctgc agatcccatt cgctatgcag atggcctacc gtttcaacgg
     5821 tatcggagtg acccagaacg tgctgtacga aaaccagaag ctgatcgcta accagttcaa
     5881 ctctgctatc ggaaagatcc aggactccct gtcctctacc gcttctgccc tgggaaagct
     5941 gcaggacgtg gtgaaccaga acgcccaggc cctgaacacc ctggtgaagc agctgtcttc
     6001 taacttcgga gccatctcct ctgtgctgaa cgacatcctg tctaggctgg acaaggtgga
     6061 agccgaggtg cagatcgaca gactgatcac cggaagactg cagtctctgc agacctacgt
     6121 gacccagcag ctgatcaggg ccgctgaaat cagagcttct gccaacctgg ctgccaccaa
     6181 gatgtccgaa tgcgtgctgg gccagtctaa gagggtggac ttctgcggaa agggatacca
     6241 cctgatgtcc ttcccacagt ctgctccaca cggagtggtg ttcctgcacg tgacctacgt
     6301 gcccgcccag gaaaagaact tcaccaccgc cccagctatc tgccacgacg gaaaggctca
     6361 cttccccagg gagggagtgt tcgtgtctaa cggaacccac tggttcgtga cccagaggaa
     6421 cttctacgag ccacagatca tcaccaccga caacaccttc gtgtctggaa actgcgacgt
     6481 ggtgatcgga atcgtgaaca acaccgtgta cgacccactg cagccagaac tggactcttt
     6541 caaggaggaa ctggacaagt acttcaagaa ccacacctct ccagacgtgg acctgggaga
     6601 catctccgga atcaacgcct ctgtggtgaa catccagaag gaaatcgaca gactgaacga
     6661 ggtggccaag aacctgaacg aatctctgat cgacctgcag gaactgggaa agtacgagca
     6721 gtacatcaag tggccctggt acatctggct gggtttcatc gctggtctga tcgctatcgt
     6781 gatggtgacc atcatgctgt gctgcatgac ctcttgctgc tcttgcctga agggatgctg
     6841 ctcttgcgga tcttgctgca agttcgacga ggacgactcc gagcctgtgc tgaagggtgt
     6901 gaagctgcac tacacctacc caactttctt gtacaaagtg gtgtaagcgg ccgccagctt
     6961 tctagaccag tttaaacatc accatcacca tcactaagtg attaacctca ggtgcaggct
     7021 gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat
     7081 cgatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
     7141 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     7201 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
     7261 ttagagtttg gcaacatatg cccatatgta actagcataa ccccttgggg cctctaaacg
     7321 ggtcttgagg ggttttttgc tgaaagcatg cggaggaaat tctccttgaa gtttccctgg
     7381 tgttcaaagt aaaggagttt gcaccagacg cacctctgtt cactggtccg gcgtattaaa
     7441 acacgataca ttgttattag tacatttatt aagcgctaga ttctgtgcgt tgttgattta
     7501 cagacaattg ttgtacgtat tttaataatt cattaaattt ataatcttta gggtggtatg
     7561 ttagagcgaa aatcaaatga ttttcagcgt ctttatatct gaatttaaat attaaatcct
     7621 caatagattt gtaaaatagg tttcgattag tttcaaacaa gggttgtttt tccgaaccga
     7681 tggctggact atctaatgga ttttcgctca acgccacaaa acttgccaaa tcttgtagca
     7741 gcaatctagc tttgtcgata ttcgtttgtg ttttgttttg taataaaggt tcgacgtcgt
     7801 tcaaaatatt atgcgctttt gtatttcttt catcactgtc gttagtgtac aattgactcg
     7861 acgtaaacac gttaaataga gcttggacat atttaacatc gggcgtgtta gctttattag
     7921 gccgattatc gtcgtcgtcc caaccctcgt cgttagaagt tgcttccgaa gacgattttg
     7981 ccatagccac acgacgccta ttaattgtgt cggctaacac gtccgcgatc aaatttgtag
     8041 ttgagctttt tggaattgcg atcgcataac ttcgtatagc atacattata cgaagttata
     8101 agctcggaac gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     8161 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8221 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8281 gccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8341 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8401 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8461 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8521 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8581 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8641 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8701 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8761 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8821 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgttac caatgcttaa
     8881 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8941 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9001 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     9061 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9121 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9181 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9241 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     9301 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9361 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9421 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9481 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9541 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9601 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9661 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9721 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatgt
     9781 ccgcgcgtt
//
