LOCUS       sequence-33621-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, 
            Eichtinger M, Jiang T, Foley JE, Winfrey RJ, Townsend JA, 
            Unger-Wallace E, Sander JD, Muller-Lerch F, Fu F, Pearlberg J, 
            Goebel C, Dassie JP, Pruett-Miller SM, Porteus MH, Sgroi DC, Iafrate
            AJ, Dobbs D, McCray PB, Cathomen T, Voytas DF, Joung JK
  TITLE     Rapid ""open-source"" engineering of customized zinc-finger 
            nucleases for highly efficient gene modification.
  JOURNAL   Mol Cell. 2008 Jul 25. 31(2):294-301.
  PUBMED    18657511
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2008 Jul 25. 31(2):294-301."
COMMENT     Sequence Label: pitx2_L (OZ533)
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..51
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     rep_origin      453..812
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(540..559)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     750..771
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 agaaaaatcc aagctttggc ggccgctgac tacaaagacg atgatgacaa atctagaccc
       61 ggggagcgcc ccttccagtg tcgcatttgc atgcggaact tttcgcggaa cgacaccctt
      121 acccggcata cccgtactca taccggtgaa aaaccgtttc agtgtcggat ctgtatgcga
      181 aatttctccc agggggggaa tttggtgcgg catctacgta cgcacaccgg cgagaagcca
      241 ttccaatgcc gaatatgcat gcgcaacttc agtgtgagca acagcctggc gaggcaccta
      301 aaaacccacc tgaggggatc ctctgcacag tagtaactag agactagaaa aaggccgaca
      361 agtcccgctc cgctgaagat cctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
      421 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
      481 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
      541 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
      601 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
      661 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
      721 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
      781 caaccctatc tcgggtctat tcttttgatt tataagggga ttttgccgaa ttttc
//
