LOCUS       sequence-33623-e         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, 
            Eichtinger M, Jiang T, Foley JE, Winfrey RJ, Townsend JA, 
            Unger-Wallace E, Sander JD, Muller-Lerch F, Fu F, Pearlberg J, 
            Goebel C, Dassie JP, Pruett-Miller SM, Porteus MH, Sgroi DC, Iafrate
            AJ, Dobbs D, McCray PB, Cathomen T, Voytas DF, Joung JK
  TITLE     Rapid ""open-source"" engineering of customized zinc-finger 
            nucleases for highly efficient gene modification.
  JOURNAL   Mol Cell. 2008 Jul 25. 31(2):294-301.
  PUBMED    18657511
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2008 Jul 25. 31(2):294-301."
COMMENT     Sequence Label: apoeb (apolipoprotein Eb)_R (OZ530)
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..75
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(564..583)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggaatacatt gtgcaaaggc cctgagaaaa atccaagctt tggcggccgc tgactacaaa
       61 gacgatgatg acaaatctag acccggggag cgccccttcc agtgtcgcat ttgcatgcgg
      121 aacttttcgg tcccgaacaa ccttagcagg catacccgta ctcataccgg tgaaaaaccg
      181 tttcagtgtc ggatctgtat gcgaaatttc tcccagcgca cccacttggt gaaccatcta
      241 cgtacgcaca ccggcgagaa gccattccaa tgccgaatat gcatgcgcaa cttcagtcag
      301 agccagcacc tggtccgcca cctaaaaacc cacctgaggg gatcctctgc acagtagtaa
      361 ctagagacta gaaaaaggcc gacaagtccc gctccgctga agatcctggc gtaatagcga
      421 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
      481 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
      541 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
      601 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
      661 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
      721 gccctgatag acggtttttc gccctttgac gtt
//
